Hepatoprotective activity of a new polyherbal formulation against paracetamol and D-galactosamine induced hepatic toxicity

Abstract

Objective:The present study was envisaged to evaluate the protective effect of polyherbal formulation, DRDC/AY/8060, developed by Dabur India Ltd., against paracetamol and D-galactosamine induced hepatic toxicities in Wistar rats.Materials and Methods:The study was carried out in two different experiments of 10 and 14 days against paracetamol and D-galactosamine, respectively. Animals were divided into different treatment groups (n = 6). The control group received normal saline, a toxicant group in two experiments received paracetamol 750 mg/kg p.o. every 72 h for 10 days and D-galactosamine 400 mg/kg i.p. single dose. The test formulation was used at the two dose levels of 120 and 240 mg/kg/day. Treatment groups treated with test formulations were also administered D-galactosamine as given in toxicant group. At the end of the dosing schedule, blood was withdrawn from the retrobulbar plexus of the animals for serum estimation of serum glutamate oxaloacetate transferase (SGOT), serum glutamate pyruvate trasnferase (SGPT), albumin, bilirubin, and alkaline phosphatase (ALP). Following the withdrawal of blood animals was sacrificed, and liver tissue was excised for estimation of thiobarbituric acid reactive substances (lipid peroxidation, malondialdehyde), tissue glutathione (GSH) and histopathological studies.Results:It was evident from the biochemical estimation that both paracetamol and galactosamine caused hepatotoxicity in the toxicant groups. However, treatment with DRDC/AY/8060 significantly (P < 0.001, vs. toxicant) reduced the levels of SGOT, SGPT, serum bilirubin, and ALP, as well as decreased lipid peroxidation. In addition, treatment with test formulation also significantly (P < 0.001, vs. toxicant) elevated serum albumin and GSH levels compared to toxicant groups.Conclusion:On the basis of these studies and comparative evaluation it can be concluded that the formulation DRDC/AY/8060 showed hepatoprotective activity against paracetamol and D-galactosamine at 120 mg/kg and 240 mg/kg.

Evaluation of the ameliorative effects of Phyllanthus niruri on the deleterious insecticide imidacloprid in the vital organs of chicken embryos

“…The PNE in group III ameliorated the altered enzyme levels; which contrasts the observations of Khan et al. [45] and Amin et al. [47] , who found the minimal inflammation and normal lobular architecture in the livers of treated rats.…”

“…Elevated liver enzymes in response to different drugs have been observed in several animal models [43] , [44] . A formulation containing the aerial parts of P. niruri has been shown to ameliorate the toxic effects of paracetamol and d -galactosamine [45] and to reduce CCl 4 -induced hepato-renal toxicity [46] . In a thioacetamide-induced liver cirrhosis Sprague Dawley rat model, P. niruri treatment improved liver biochemical parameters, reduced oxidative stress enzyme and lipid peroxidation levels along, and restored histological and morphological patterns in livers [47] , as well as in diabetic rats [48] .…”

Protective Effects of Chrysin Against Drugs and Toxic Agents

“…D-galactosamine (GalN) is known as a hepatotoxic chemical agent. 50 D-galactosamine increased serum hepatic marker enzyme activities (AST, ALT, alkaline phosphatase, and γ-glutamyl transpeptidase) and the lipid peroxidation and decreased the antioxidant capacity of the plasma and liver. It was observed that CH decreased hepatic enzyme activities and lipid peroxidation products, such as TBARS, lipid hydroperoxides, and conjugated dienes, and increased the activities of free radical–scavenging enzymes, such as SOD, CAT, GPx, GR, GST, and the levels of GSH, vitamin C and vitamin E in the liver.…”

Evaluation of the acute basic biological effects of herbal formulation to control obesity: a preliminary study

“…Even though the activity of the body increased due to metabolic and circulatory alterations occur when the tissues are damaged (Ma, 2004). Polyherbal formulation contains the andrographolide in steam and leaves which show the anti‐hepatotoxic activity and also reduces the level of CCL4‐induced hepatic enzymes (Khan et al ., 2015). Flavone and flavonoid concentrates present in polyherbal formulation give hepatoprotective property which maintain the level of serum glutamate pyruvate transaminase, alkaline phosphatase, serum glutamate oxaloacetate transaminase, and direct and indirect bilirubin (Gupta et al ., 2013).…”