Hepatoprotection by carotenoids in isoniazid–rifampicin induced hepatic injury in rats

Abstract

This study evaluates the hepatoprotective effect of carotenoids against isoniazid (INH) and rifampicin (RIF). Thirty-six adult rats were divided into the following 4 groups: (1) control group treated with normal saline; (2) INH + RIF group treated with 50 mg·(kg body mass)-1·day-1 of INH and RIF each; (3) INH + RIF+ carotenoids group treated with 50 mg·(kg body mass)-1·day-1 of INH and RIF each and 10 mg·(kg body mass)-1·day-1 of carotenoids; and (4) carotenoids group treated with 10 mg·(kg body mass)-1·day-1 of carotenoids for 28 days intragastrically. Oxidative stress and antioxidant levels in liver and blood, liver histology and change in transaminases were measured in all the above-mentioned groups. There was an increase in lipid peroxidation with a reduction in thiols, catalase, and superoxide dismutase (SOD) in the liver and blood of rats accompanied by an increase in transaminases, bilirubin, and alkaline phosphatase. Treatment with carotenoids along with INH + RIF partially reversed lipid peroxidation, thiols, catalase, and SOD in the liver and blood of rats. Elevated levels of the enzymes in serum were also reversed partially by this treatment. The degree of necrosis, portal triaditis, and inflammation were also lowered in the carotenoids group. In conclusion, carotenoids supplementation in INH + RIF treated rats showed partial protection.

Cytoprotective Effects of Taurine Against Toxicity Induced by Isoniazid and Hydrazine in Isolated Rat Hepatocytes

“…Hydrazine is the product of cytochrome P450 metabolising activity (5) and is believed to induce oxidative stress (6). One of the consequences of oxidative stress is lipid peroxidation, as established in animal models treated with anti-tuberculosis drugs such as isoniazid (7) or hydrazine (8). Another consequence, established by Chowdhury et al (9), is mitochondrial damage in hepatocytes.…”

Metabolomics for the Quality Assessment ofLycium chinenseFruits

“…23,24) The hepato-protective properties of carotenoids and tocopherols have also been demonstrated in recent studies. 25,26) These effects were achieved when carotenoids and -tocopherols were respectively treated every single day with 10 mg for 6 weeks and 200 mg for 4 weeks per 1 kg body mass of rats. The LM-3 cultivar therefore had more potential as an antioxidant, at least in hepatocytes, than the other cultivars.…”

Comparison between acetylator phenotype and genotype polymorphism of n-acetyltransferase-2 in tuberculosis patients

“…Difference in N-acetylation can be a result of variant NAT2 alleles that produce fast and slow acetylator phenotypes and has been associated with various cancers [18]. Hepatoprotection by carotenoids in ''INH-and RIF''-induced hepatic injury in rats showed partial protection [19]. Over the past 40 years, many techniques have been devised to determine the acetylator status phenotypically, but most of those techniques did not give an unequivocal characterization of the phenotype [20].…”