Hepatopancreas Transcriptome Profiling Analysis Reveals Physiological Responses to Acute Hypoxia and Reoxygenation in Juvenile Qingtian Paddy Field Carp Cyprinus carpio var qingtianensis

Qi, Ming; Wu, Qianqian; Liu, Tao; Hou, Yiling; Miao, Yixin; Hu, Menghong; Liu, Qigen

doi:10.3389/fphys.2020.01110

Cited by 19 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We speculate that this may inhibit glucose consumption in GIFT, and may result in hypoxia-induced damage to GIFT by triggering inflammation and insulin resistance. These results confirmed that in GIFT under hypoxia stress, anaerobic glucose metabolism is weakened and glucose homeostasis is imbalanced, which may be related to the increased death rate at 96 h. The metabolome analyses revealed increased contents of D-gluconate, N-acetyl-D-glucosamine, and N-acetyl-D-glucosamine-6-phosphate in the pentose phosphate pathway, indicating that this pathway was activated under acute hypoxia stress, similar to the results reported elsewhere [52]. Besides providing energy, the pentose phosphate pathway is also the main source of NADPH and ribose in the body [53], and it can provide raw materials for the synthesis of fatty acids, ribonucleic acids, and cholesterol.…”

Section: Discussionsupporting

confidence: 90%

“…The metabolome analyses revealed increased contents of D-gluconate, N-acetyl-D-glucosamine, and N-acetyl-D-glucosamine-6-phosphate in the pentose phosphate pathway, indicating that this pathway was activated under acute hypoxia stress, similar to the results reported elsewhere [ 52 ]. Besides providing energy, the pentose phosphate pathway is also the main source of NADPH and ribose in the body [ 53 ], and it can provide raw materials for the synthesis of fatty acids, ribonucleic acids, and cholesterol.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Qiang

Tao

et al. 2021

BMC Genomics

View full text Add to dashboard Cite

Background Dissolved oxygen (DO) in the water is a vital abiotic factor in aquatic animal farming. A hypoxic environment affects the growth, metabolism, and immune system of fish. Glycolipid metabolism is a vital energy pathway under acute hypoxic stress, and it plays a significant role in the adaptation of fish to stressful environments. In this study, we used multi-omics integrative analyses to explore the mechanisms of hypoxia adaptation in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). Results The 96 h median lethal hypoxia (96 h-LH50) for GIFT was determined by linear interpolation. We established control (DO: 5.00 mg/L) groups (CG) and hypoxic stress (96 h-LH50: 0.55 mg/L) groups (HG) and extracted liver tissues for high-throughput transcriptome and metabolome sequencing. A total of 581 differentially expressed (DE) genes and 93 DE metabolites were detected between the CG and the HG. Combined analyses of the transcriptome and metabolome revealed that glycolysis/gluconeogenesis and the insulin signaling pathway were down-regulated, the pentose phosphate pathway was activated, and the biosynthesis of unsaturated fatty acids and fatty acid metabolism were up-regulated in GIFT under hypoxia stress. Conclusions The results show that lipid metabolism became the primary pathway in GIFT under acute hypoxia stress. Our findings reveal the changes in metabolites and gene expression that occur under hypoxia stress, and shed light on the regulatory pathways that function under such conditions. Ultimately, this information will be useful to devise strategies to decrease the damage caused by hypoxia stress in farmed fish.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Qiang

Tao

et al. 2021

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Dissolved oxygen in the water directly affects energy metabolism, blood parameters, and physiological defense against oxidative stress in fishes, et al ( Wu, 2002 ). In fish, liver (PF-carp for hepatopancreas) are critical to metabolism ( Guilherme et al, 2012 ; Qi M et al, 2020 ). Changes in dissolved oxygen affect the physiological function of liver and disturb energy metabolism, antioxidant stress and the synthesis of other substances ( Almeida-Val et al, 2000 ; Wang and Richards, 2011 ; Jung et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Profiling Analysis of Physiological Responses to Acute Hypoxia and Reoxygenation in Juvenile Qingtian Paddy Field Carp Cyprinus Carpio Var Qingtianensis

Jiang

Qi²,

Zhang

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

The Qingtian paddy field carp (Cyprinus carpio var qingtianensis) is a local carp cultivated in the rice field of Qingtian county, Zhejiang province, China. The paddy field environment is distinct from the pond environment. Due to the inability to artificially increase oxygen, the dissolved oxygen greatly changes during the day. Therefore, investigating the physiological regulation to the changes of acute dissolved oxygen in Qingtian paddy field carp (PF-carp) will dramatically clarify how it adapts to the paddy breeding environment. The high tolerance of Qingtian paddy field carp to hypoxia makes it an ideal organism for studying molecular regulatory mechanisms during hypoxia process and reoxygenation following hypoxia in fish. In this study, we compared the changes of metabolites in the hepatopancreas during hypoxia stress and the following reoxygenation through comparative metabolomics. The results showed 131 differentially expressed metabolites between the hypoxic groups and control groups. Among them, 95 were up-regulated, and 36 were down-regulated. KEGG Pathway enrichment analysis showed that these differential metabolites were mainly involved in regulating lipid, protein, and purine metabolism PF-carps could require energy during hypoxia by enhancing the gluconeogenesis pathway with core glutamic acid and glutamine metabolism. A total of 63 differentially expressed metabolites were screened by a comparison between the reoxygenated groups and the hypoxic groups. Specifically, 15 were up-regulated, and 48 were down-regulated. The KEGG Pathway enrichment analysis supported that PF-carp could continue to gain energy by consuming glutamic acid and the glutamine accumulated during hypoxia and simultaneously weaken the ammonia-transferring effect of amino acids and the toxicity of ammonia. By consuming glycerophospholipids and maintaining the Prostaglandin E content, cell damage was improved, sphingosinol synthesis was reduced, and apoptosis was inhibited. Additionally, it could enhance the salvage synthesis and de novo synthesis of purine, reduce purine accumulation, promote the synthesis of nucleotide and energy carriers, and assist in recovering physiological metabolism. Overall, results explained the physiological regulation mechanism of PF-carp adapting to the acute changes of dissolved oxygen at the metabolic level and also provided novel evidence for physiological regulation of other fish in an environment with acute changes in dissolved oxygen levels.

show abstract

“…For instance, the induction of hypoxia and thermal responsive genes in fish exposed to either water temperature and hypoxia stresses remains upregulated hours after returning to CTs and normal oxygen (i.e. 6–8 h; Buckley et al , 2006 ; Qi et al , 2020 ). Using molecular biomarkers specific to hypoxic and thermal stresses independently, we have demonstrated that during summer and early fall months when Cultus Lake exhibits profundal hypoxia and high water temperatures in the epilimnion, over one-third of the fish sampled were responding to hypoxic and thermal stresses concurrently.…”

Section: Discussionmentioning

confidence: 99%

Endangered Cultus Lake sockeye salmon exhibit genomic evidence of hypoxic and thermal stresses while rearing in degrading freshwater lacustrine critical habitat

Akbarzadeh

Selbie

Pon

et al. 2021

Conservation Physiology

View full text Add to dashboard Cite

Water quality degradation due to lake eutrophication and climate change contributes to the risk of extirpation for the endangered Cultus Lake sockeye salmon. Sockeye salmon juveniles experience both low-oxygen water in profundal lake habitats and elevated temperatures above the thermocline during diel vertical migrations in summer and fall when the lake is thermally stratified. We used a transcriptomic tool (Salmon Fit-Chip) to determine whether salmon were experiencing thermal and/or hypoxic stress during this period. The results showed that over one-third of the fish were responding to either hypoxic (35.5%) or thermal stress (40.9%) during periods when these environmental stressors were pronounced within the lake, but not during periods when profundal dissolved oxygen was elevated and the water column was isothermal and cool. The most consistent signs of hypoxic stress occurred during July (52.2%) and September (44.4%). A total of 25.7% of individual fish sampled during months when both stressors were occurring (July, September, October) showed signatures of both stressors. When a combination of hypoxic and thermal stress biomarkers was applied, 92% of fish showed evidence of one or both stressors; hence, for at least several months of the year, most sockeye salmon juveniles in Cultus Lake are experiencing anthropogenically environmentally induced stress. We also detected the presence of pathogenic ciliate Ichthyoptherius multifiliis in the gill tissue of juveniles, with a higher infection signal in Cultus Lake compared to juveniles from nearby Chilliwack Lake. These data provide powerful new evidence that Cultus Lake sockeye salmon, which experience relatively lower juvenile survival than Chilliwack sockeye salmon, are more compromised by stress and carry a higher level of infection of at least one pathogenic agent. Thus, we hypothesize that the cumulative or synergistic interplay between stressors and diseases, clearly documented to be occurring within Cultus Lake, are contributing to increased mortality of endangered sockeye salmon.

show abstract

Hepatopancreas Transcriptome Profiling Analysis Reveals Physiological Responses to Acute Hypoxia and Reoxygenation in Juvenile Qingtian Paddy Field Carp Cyprinus carpio var qingtianensis

Cited by 19 publications

References 61 publications

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Metabolomic Profiling Analysis of Physiological Responses to Acute Hypoxia and Reoxygenation in Juvenile Qingtian Paddy Field Carp Cyprinus Carpio Var Qingtianensis

Endangered Cultus Lake sockeye salmon exhibit genomic evidence of hypoxic and thermal stresses while rearing in degrading freshwater lacustrine critical habitat

Contact Info

Product

Resources

About