Hepatopancreas cell cultures from mud crab, Scylla paramamosain

Zeng, Hui; Ye, Haihui; Li, Shaojing; Wang, Guizhong; Huang, Jingru

doi:10.1007/s11626-009-9259-5

Cited by 29 publications

(20 citation statements)

References 36 publications

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“…However, in spite of their inherent physiological importance, the current knowledge about forms, modulation by environmental factors, and biochemical features of amylase in the hepatopancreas (the main site of digestive enzyme synthesis and of digestion and absorption of nutrients and storage of energy substrates) of groups of ecological importance such as intertidal euryhaline crabs is still scarce and fragmentary (Blandamer and Beechey, 1966;Van Wormhoudt et al, 1995;Asaro et al, 2011;Zeng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K + , Li + , Co 2+ , and Mg 2+ (36%-45%); inhibited by Cu 2+ , Zn 2+ , Cd 2+ , Fe 2+ , and Mn 2+ (92.4% and 23.7%); not affected by Ni 2+ or Ba 2+ ; and enhanced almost 100% by Ca 2+ . Amylase exhibited Michaelis-Menten kinetics (starch: K m = 1.24 mg mL -1 ; glycogen: K m = 16.19 mg mL -1 ). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

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Section: Introductionmentioning

confidence: 99%

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Asaro¹,

Paggi²,

Castro³

et al. 2017

Turk J Zool

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show abstract

“…Primary cell cultures from different shrimp tissues such as lymphoid organ (Chen and Kou 1989;Chen and Wang 1999;Wang et al 2000), heart (Chen and Wang 1999; Owens and Smith 1999), gut (Nadala et al 1993), ovaries (Maeda et al 2004) have been used to study various viral infections and replications but long surviving and proliferating cell cultures were not then available for evaluating viral infections. Though, few researchers have attempted primary cell cultures of crabs (Ballard et al 1993;Walton and Smith 1999;Sashikumar and Desai 2008;Zeng et al 2009) none have used crabs' cell culture for studying WSSV pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

Shashikumar

Desai

2012

Cytotechnology

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Testicular cell culture of crab, Scylla serrata (Forskal) was used to study the effects of White spot syndrome virus (WSSV). We are showing the susceptibility of cell culture of crabs to WSSV. The proliferating cell culture of testes were maintained for more than 4 months in a medium prepared from L15 and crab saline supplemented with epidermal growth factor. The cell cultures inoculated with different concentrations of virus showed distinct cytopathic effects such as change in cell appearance, shrinkage and cell lysis. WSSV infection of cultured cells was confirmed by Nested PCR technique. The incorporation of viral DNA in cultured cells was shown by RAPD profile generated using 10-mer primers. The controls that were not exposed to WSSV did not show cytopathic effects. This work shows the usefulness of proliferating testicular cell culture for studying WSSV infection using molecular tools. Thus, this report gains significance as it opens new vistas for diagnostics and drugs for WSSV.

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“…The crab hepatopancreas (midgut gland) is an extremely large organ with several functions, including the secretion of digestive enzymes, and absorption and storage of digested food (Zeng et al, 2010). The highest levels of expression of all the three GILT-like genes were in this organ (Fig.…”

Section: Function Of Gilt Isoforms In Mud Crabs and Other Invertebratesmentioning

confidence: 99%

Identification of three IFN-γ inducible lysosomal thiol reductase ( GILT )-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression

Huang

Duan

Huang

et al. 2015

Gene

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Hepatopancreas cell cultures from mud crab, Scylla paramamosain

Cited by 29 publications

References 36 publications

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Amylase in the hepatopancreas of a euryhaline burrowingcrab: characteristics and modulation

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

Identification of three IFN-γ inducible lysosomal thiol reductase ( GILT )-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression

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