Hepatogenesis of murine induced pluripotent stem cells in 3D micro-cavitary hydrogel system for liver regeneration

Lau, Ting Ting; Ho, Li Wen; Wang, Dong‐An

doi:10.1016/j.biomaterials.2013.05.034

Cited by 41 publications

(57 citation statements)

References 31 publications

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“…79 Primary hepatocyte is probably the preferred cell for cell therapy in liver regeneration. However, its non-ideal proliferation capacity and rapid loss of phenotype during 2D culture compromises the quality and quantity of the transplanted hepatocytes, resulting in variable success rates of this treatment.…”

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

“…For widening the clinical applications dual-functioning genipin crosslinked gelatin microspheres are which serve as cell carriers as well as porogens for delivering the model cells and also for creating cavitie. 79 The cells were first seeded onto genipin cross linked gelatin microspheres for attachment, followed by encapsulation in alginate hydrogel. 79 Collagenase, MMP-9, is used either in the culture media or mixed with alginate precursor solution to allow microsphere degradation for creating cavities within the gel bulk.…”

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

“…79 The cells were first seeded onto genipin cross linked gelatin microspheres for attachment, followed by encapsulation in alginate hydrogel. 79 Collagenase, MMP-9, is used either in the culture media or mixed with alginate precursor solution to allow microsphere degradation for creating cavities within the gel bulk. Accordingly, the cells proliferate within the cavities, forming hepatocellular aggregates while the alginate hydrogel serves as a confinement, restricting the size and the shape of the aggregates to the size of the cavities.…”

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

“…Accordingly, the cells proliferate within the cavities, forming hepatocellular aggregates while the alginate hydrogel serves as a confinement, restricting the size and the shape of the aggregates to the size of the cavities. 79 Statins also showed therapeutic effect of on bone formation and neo vascularization. But its low dose systemic administration is limited because it causes adverse side effects on liver metabolism and clearance in the digestive system.…”

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

“…92 These are also used for production of autologous stem cells from terminally differentiated somatic cells. 79 Similarly, combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cellderived endothelial cells in state of hypoxi. 93 Induced pluripotent stem cells (iPSCs) are used to treat acute hepatic failure (AHF) which is a severe liver injury leading to sustained damage and complications.…”

mentioning

confidence: 99%

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Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Present review article emphasizes role of biological scaffolds, hydrogels and stem cells in tissue engineering mainly in regeneration or repairing of damaged tissues. Highly porous scaffold biomaterials are developed which act as templates for tissue regeneration and potentially guide the growth of new tissue. Present article also describes de-cellularization, cell printing, vascularization, integration of cross linking of methods for scaffolding of biomaterials to reproduce and recapitulate complexity in engineered tissues. It also explains different scaffold types, polymer hydrogels which are necessary for formation of microstructure, cell attachment, differentiation, tissue vascularization and integration. It also explains use of induced pluripotent stem cells (iPSCs) and engineered MSCs as new diagnostic and potential therapeutic tools to remove sustained damage and complications from organ failures. These engineered MSCs assist in making self-assembling supramolecular hydrogels, which have larger applications in cell therapy of intractable diseases and tissue regeneration. No doubt development of more advanced biomaterials, growth factors and stem cell derived products/factors and tissue transplantation methods based on cell regeneration programming will revolutionize the clinical therapeutics. This article suggests identification of new biomaterials/biomolecules such as growth factors, scaffolds, integration, adhesion and regulatory molecules and cell secreted factors which can induce major metabolic and signaling pathways during phase of tissue repairing and induction of regeneration. This innovative research area needs many conceptual improvements in organ therapies, methods and technological advancement to scale more services to the human society.

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Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

Section: Cross Linking Of Biomaterialsmentioning

confidence: 99%

mentioning

confidence: 99%

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Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Engineering Liver Microtissues for Disease Modeling and Regenerative Medicine

Huang

Gibeley

et al. 2020

Adv Funct Materials

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The burden of liver diseases is increasing worldwide, accounting for two million deaths annually. In the past decade, tremendous progress has been made in the basic and translational research of liver tissue engineering. Liver microtissues are small, 3D hepatocyte cultures that recapitulate liver physiology and have been used in biomedical research and regenerative medicine. This review summarizes the recent advances, challenges, and future directions in liver microtissue research. Cellular engineering approaches are used to sustain primary hepatocytes or produce hepatocytes derived from pluripotent stem cells and other adult tissues. 3D microtissues are generated using scaffoldfree assembly or scaffold-assisted methods such as macroencapsulation, droplet microfluidics, and bioprinting. Optimization of the hepatic microenvironment entails incorporating the appropriate cell composition for enhanced cell-cell interactions and niche-specific signals, and creating scaffolds with desired chemical, mechanical, and physical properties. Perfusion-based culture systems such as bioreactors and microfluidic systems are used to achieve efficient exchange of nutrients and soluble factors. Taken together, systematic optimization of liver microtissues is a multidisciplinary effort focused on creating liver cultures and on-chip models with greater structural complexity and physiological relevance for use in liver disease research, therapeutic development, and regenerative medicine.

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Biomaterials and Culture Technologies for Regenerative Therapy of Liver Tissue

Pérez

Jung

Kim

2016

Adv Healthcare Materials

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Regenerative approach has emerged to substitute the current extracorporeal technologies for the treatment of diseased and damaged liver tissue. This is based on the use of biomaterials that modulate the responses of hepatic cells through the unique matrix properties tuned to recapitulate regenerative functions. Cells in liver preserve their phenotype or differentiate through the interactions with extracellular matrix molecules. Therefore, the intrinsic properties of the engineered biomaterials, such as stiffness and surface topography, need to be tailored to induce appropriate cellular functions. The matrix physical stimuli can be combined with biochemical cues, such as immobilized functional groups or the delivered actions of signaling molecules. Furthermore, the external modulation of cells, through cocultures with nonparenchymal cells (e.g., endothelial cells) that can signal bioactive molecules, is another promising avenue to regenerate liver tissue. This review disseminates the recent approaches of regenerating liver tissue, with a focus on the development of biomaterials and the related culture technologies.

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Hepatogenesis of murine induced pluripotent stem cells in 3D micro-cavitary hydrogel system for liver regeneration

Cited by 41 publications

References 31 publications

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Engineering Liver Microtissues for Disease Modeling and Regenerative Medicine

Biomaterials and Culture Technologies for Regenerative Therapy of Liver Tissue

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