Hepatocytes derived from peripheral blood stem cells of granulocyte-colony stimulating factor treated F344 rats in analbuminemic rat livers1

Granulocyte–Colony Stimulating Factor Promotes Liver Repair and Induces Oval Cell Migration and Proliferation in Rats

“…17 G-CSF stimulated peripheral blood BMCs to give rise to albumin-producing hepatocytes after infusion into analbuminemic rats. 18 Finally, G-CSF administration was able to induce BMC mobilization in cirrhotic patients, and a clinical improvement was registered in about 50% of treated individuals. 25 However, it remains unclear if G-CSF acts mainly by recruiting BMCs, or if it acts locally to facilitate the endogenous hepatic restoration program.…”

“…14 Recent studies have suggested that G-CSF may be effective in mobilizing cells that contribute to liver repair after damage. [15][16][17][18] However, it remains unclear if G-CSF acts mainly by recruiting BMCs, or if it acts locally within the liver microenvironment to facilitate the endogenous hepatic restoration program. The potential trophic effects of G-CSF on liver stem cells could have a dramatic clinical impact because G-CSF is one of the few growth factors approved for use in patients.…”

Analbuminemic Rat Model for Hepatocyte Transplantation

“…Because of the 7-base-pair deletion in the 9th intron of the analbuminemic albumin gene, PCR amplification of amplicons spanning these sequences can differentially detect normal and abnormal genes, which can be used to quantify the expression of the normal albumin gene, using the abnormal albumin gene as an internal control (Ogawa et al, 1993). This method is applicable to hepatocytes and other cell types, such as bone marrow cells (Arikura et al, 2004;Inagaki et al, 2011).…”

“…This process is thought mediated by the direct transdifferentiation of bone marrow cells into hepatocytes (Theise et al, 2000), the indirect transdifferentiation of bone marrow cells into oval cells that have the potential to give rise to mature hepatocytes during the cholangiocellular lineage (Oh et al, 2007), and the transfer of genetic materials from bone marrow cells to recipient hepatocytes by cell fusion (Wang et al, 2003). Using the analbuminemic rat model, we investigated whether bone marrow cells gave rise to hepatocytes during post-PH liver regeneration (Arikura et al, 2004). In this process, the original hepatic mass is mainly regenerated by the division of resident hepatocytes.…”

“…In a female F344-alb rat that had been transplanted with male F344 bone marrow cells, albumin-positive hepatocyte clusters in the liver were positive for the Y chromosome marker Sry3 (Figure 4D a, b). We also investigated whether hematopoietic stem cells mobilized from the bone marrow into the peripheral blood can give rise to hepatocytes in the regenerating liver after PH (Huiling et al, 2004). The donor F344 rats were repeatedly treated with the recombinant granulocyte colony stimulating factor (G-CSF).…”