Hepatocyte Nuclear Factors-1α, -1β, and -3β Expressed in the Gonad of Tilapia (Oreochromis mossambicus)

Expression and in vitro regulation of pituitary adenylate cyclase-activating polypeptide (pacap38) and its type I receptor (pac1-r) in the gonads of tilapia (Oreochromis mossambicus)

“…Total RNA was extracted from the organs with RNazol (Tel-Test, Friendswood, TX, USA) following the instructions from the manufacturer. cDNA was synthesized using an RT-PCR system (SuperScript II Reverse Transcriptase, Invitrogen) as described previously (Huang et al 2001(Huang et al , 2007. Gene-specific primers for the coding region of tpacap 38 (GenBank accession no.…”

“…AY830121), and b-actin (GenBank accession no. AB037865) used in the PCR were as described previously (Huang et al 2001, Lo et al 2007. b-actin expression served as an internal control for normalization.…”

“…The PCR products were analyzed on a 1.2% agarose gel and were sequenced directly. Nucleic acid and putative amino acid sequences were compared with all published sequences on the genetic computer program (Huang et al 2001(Huang et al , 2007.…”

“…The signal of b-actin was used as an internal control and normalized with every band in the same treatment for calculation and comparison with other treatments. The relative expression level of tpacap 38 and tpac 1 -r in each experiment was expressed as the ratio of tpacap 38 and tpac 1 -r mRNA to b-actin mRNA (Huang et al 2001(Huang et al , 2007. The results of control groups (0 h in endogenous expression experiment, and testes or ovarian follicles cultured for 8 h without supplement in the following time course experiments or for additional 2 h without Figure 9 Effects of forskolin on the expression of tpac 1 -r in male (A and C) and female (B and D) gonads in the absence or presence of H89 using semiquantitative RT-PCR analysis.…”

Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1α/tcf1 and hnf1β/tcf2, abundantly expressed in liver, pancreas, gut and kidney of zebrafish

“…Apart from its functional roles in adult organs, hnf1b is required for embryonic development including visceral endoderm, ectoderm differentiation (Coffinier et al, 1999), mesoderm induction (Vignali et al, 2000) and organogenesis of kidney, liver and pancreas (Wild et al, 2000;Sun and Hopkins, 2001). In teleosts, only one hnf1 form was reported in Atlantic salmon (Deryckere et al, 1995) and two hnf1 partial cDNAs in tilapia were identified by us (Huang et al, 2001). In zebrafish, conserved vhnf1/hnf1b, which is involved in kidney cysts formation similar to human symptoms of hnf1b-associated human familial glomerulocystic kidney disease (GCKD) and in controlling development of multiple organs through regulating regional specification of organ primordia, was identified by retroviral insertion mutagenesis (Sun and Hopkins, 2001).…”