Hepatocyte Growth Factor Induces Proliferation of Lens Epithelial Cells through Activation of ERK1/2 and JNK/SAPK

Choi, Jinah; Park, Sun Young; Joo, Choun‐Ki

doi:10.1167/iovs.03-1371

Cited by 55 publications

(35 citation statements)

References 40 publications

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“…While characterization of growth factors in the aqueous humour is incomplete, various growth factors have been identified and shown to play similar or overlapping roles in lens cells. FGF [8,126], PDGF [127,128], IGF [30,129], hepatocyte growth factor (HGF) [130,131], TGF-a [132] and EGF [133] are some of the mitogenic growth factors found in aqueous. Studies in lens epithelial explants have shown that aqueous-induced proliferation is dependent on MAPK/ERK1/2 and PI3-K/Akt signalling [21,22,25].…”

Section: Formation Maintenance and Proliferation Of The Lens Epitheliummentioning

confidence: 99%

Understanding the role of growth factors in embryonic development: insights from the lens

Lovicu

McAvoy

Iongh

2011

Phil. Trans. R. Soc. B

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Growth factors play key roles in influencing cell fate and behaviour during development. The epithelial cells and fibre cells that arise from the lens vesicle during lens morphogenesis are bathed by aqueous and vitreous, respectively. Vitreous has been shown to generate a high level of fibroblast growth factor (FGF) signalling that is required for secondary lens fibre differentiation. However, studies also show that FGF signalling is not sufficient and roles have been identified for transforming growth factor-b and Wnt/Frizzled families in regulating aspects of fibre differentiation. In the case of the epithelium, key roles for Wnt/b-catenin and Notch signalling have been demonstrated in embryonic development, but it is not known if other factors are required for its formation and maintenance. This review provides an overview of current knowledge about growth factor regulation of differentiation and maintenance of lens cells. It also highlights areas that warrant future study.

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Section: Formation Maintenance and Proliferation Of The Lens Epitheliummentioning

confidence: 99%

Understanding the role of growth factors in embryonic development: insights from the lens

Lovicu

McAvoy

Iongh

2011

Phil. Trans. R. Soc. B

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“…In addition, mitogenic signals, including growth factors [21] and CD40 ligation [22] can induce the activation of JNK, …”

Section: Effects Of Pcf On the Expression Of Cleaved Caspase-3 Inducementioning

confidence: 99%

Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB

Gao

Guo

Chen

et al. 2007

Acta Pharmacologica Sinica

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Aim: To investigate the mechanism of polypeptide from Chlamys farreri (PCF) protecting HaCaT cells from apoptosis induced by UVA plus UVB in vitro. Methods: An apoptotic model of UV irradiation-induced HaCaT cells was established. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, agarose gel electrophoresis, biochemical methods, and Western blotting were employed in the study. Results: PCF inhibited the UV irradiation-induced apoptosis of HaCaT cells. PCF strongly reduced the intracellular reactive oxygen species level, enhanced activities of superoxide dismutase and glutathione peroxidase and increased the total anti-oxidative capacity in HaCaT cells following UV irradiation. Furthermore, we found that PCF could inhibit the phosphorylation of c-Jun amino-terminal kinase and the activity of caspase-3 in a concentrationdependent manner. Conclusion: PCF protected HaCaT cells from apoptosis induced by UVA plus UVB, mainly through decreasing the intracellular ROS level and increasing the activities of anti-oxidative enzymes to block the ROS-JNKcaspase-3-apoptosis signaling pathway.

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“…Although several RTK signaling factors, including PDGF, IGF, EGF and HGF, can promote lens cell proliferation in vitro, only FGFs can induce lens cell differentiation in mammals (Choi et al, 2004;Hyatt and Beebe, 1993;Kok et al, 2002;Liu et al, 1996;McAvoy and Chamberlain, 1989;Reddan and Wilson-Dziedzic, 1983;Wormstone et al, 2000). In transgenic mice, misexpression of the secreted forms of Fgf1,3,4,7,8 or 9 led to premature differentiation of lens epithelial cells into fiber cells, whereas dominant-negative Fgfr1 suppressed lens epithelial cell proliferation (Faber et al, 2001;Lovicu and Overbeek, 1998;Robinson et al, 1998;Robinson et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Sprouty2-modulated Kras signaling rescues Shp2 deficiency during lens and lacrimal gland development

et al. 2010

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SUMMARYShp2/Ptpn11 tyrosine phosphatase is a general regulator of the RTK pathways. By genetic ablation, we demonstrate that Shp2 is required for lacrimal gland budding, lens cell proliferation, survival and differentiation. Shp2 deletion disrupted ERK signaling and cell cycle regulation, which could be partially compensated by activated Kras signaling, confirming that Ras signaling was the main downstream target of Shp2 in lens and lacrimal gland development. We also showed that Sprouty2, a general suppressor of Ras signaling, was regulated by Shp2 positively at the transcriptional level and negatively at the post-translational level. Only in the absence of Sprouty2 could activated Kras signaling robustly rescue the lens proliferation and lacrimal-gland-budding defects in the Shp2 mutants. We propose that the dynamic regulation of Sprouty by Shp2 might be important not only for modulating Ras signaling in lens and lacrimal gland development, but also for RTK signaling in general.

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Hepatocyte Growth Factor Induces Proliferation of Lens Epithelial Cells through Activation of ERK1/2 and JNK/SAPK

Abstract: The data indicate that HGF is a potent growth factor for LECs and may contribute to the development of PCO and suggest that the signaling pathways involved in HGF-stimulated proliferation may constitute potential therapeutic targets in the treatment of PCO.

Cited by 55 publications

References 40 publications

Understanding the role of growth factors in embryonic development: insights from the lens

Understanding the role of growth factors in embryonic development: insights from the lens

Molecular mechanisms of polypeptide from Chlamys farreri protecting HaCaT cells from apoptosis induced by UVA plus UVB

Sprouty2-modulated Kras signaling rescues Shp2 deficiency during lens and lacrimal gland development

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