Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease

Wilczyńska-Borawska, Magdalena; Borawski, Jacek; Kovalchuk, Oksana; Chyczewski, L; Stokowska, W

doi:10.2334/josnusd.48.47

Cited by 15 publications

(8 citation statements)

References 14 publications

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“…Exceptionally, 3 independent studies demonstrated significant association of salivary HGF with periodontitis [119–122] which is consistent with similar data analysing GCF [123]. In terms of function, HGF is known to be involved in dental development [124] but, significantly, HGF is secreted by gingival fibroblasts and HGF secretion is regulated by cytokines and bacterial products; it has been hypothesised that HGF may mediate epithelial apical migration in periodontitis [124, 125].…”

Section: Protein Biomarkers Of Periodontitis: Candidate Protein Stsupporting

confidence: 62%

Protein Biomarkers of Periodontitis in Saliva

Taylor

2014

ISRN Inflammation

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Periodontitis is a chronic inflammatory condition of the tissues that surround and support the teeth and is initiated by inappropriate and excessive immune responses to bacteria in subgingival dental plaque leading to loss of the integrity of the periodontium, compromised tooth function, and eventually tooth loss. Periodontitis is an economically important disease as it is time-consuming and expensive to treat. Periodontitis has a worldwide prevalence of 5–15% and the prevalence of severe disease in western populations has increased in recent decades. Furthermore, periodontitis is more common in smokers, in obesity, in people with diabetes, and in heart disease patients although the pathogenic processes underpinning these links are, as yet, poorly understood. Diagnosis and monitoring of periodontitis rely on traditional clinical examinations which are inadequate to predict patient susceptibility, disease activity, and response to treatment. Studies of the immunopathogenesis of periodontitis and analysis of mediators in saliva have allowed the identification of many potentially useful biomarkers. Convenient measurement of these biomarkers using chairside analytical devices could form the basis for diagnostic tests which will aid the clinician and the patient in periodontitis management; this review will summarise this field and will identify the experimental, technical, and clinical issues that remain to be addressed before such tests can be implemented.

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Section: Protein Biomarkers Of Periodontitis: Candidate Protein Stsupporting

confidence: 62%

Protein Biomarkers of Periodontitis in Saliva

Taylor

2014

ISRN Inflammation

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“…HGF is also a healing factor, with cytoprotective effects in conditions associated with inflammatory diseases, including periodontitis. A number of studies demonstrated that HGF levels were elevated in patients with chronic periodontitis [ 23 , 24 ]. However, despite high concentrations, HGF may appear in a form with reduced biological activity at local inflammation sites [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine

et al. 2015

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IntroductionPeriodontitis is one of the most widespread infectious diseases in humans. We previously promoted significant periodontal tissue regeneration in swine models with the transplantation of autologous periodontal ligament stem cells (PDLSCs) and PDLSC sheet. We also promoted periodontal tissue regeneration in a rat model with a local injection of allogeneic bone marrow mesenchymal stem cells. The purpose of the present study is to investigate the roles of the hepatocyte growth factor (HGF) and human dental pulp stem cells (DPSCs) in periodontal tissue regeneration in swine.MethodIn the present study, we transferred an adenovirus that carried HGF gene into human DPSCs (HGF-hDPSCs) under good manufacturing practice (GMP) conditions. These cells were then transplanted into a swine model for periodontal regeneration. Twenty miniature pigs were used to generate periodontitis with bone defect of 5 mm in width, 7 mm in length, and 3 mm in depth. After 12 weeks, clinical, radiological, quantitative and histological assessment of regenerated periodontal tissues was performed to compare periodontal regeneration in swine treated with cell implantation.ResultsOur study showed that injecting HGF-hDPSCs into this large animal model could significantly improve periodontal bone regeneration and soft tissue healing. A hDPSC or HGF-hDPSC sheet showed superior periodontal tissue regeneration compared to the injection of dissociated cells. However, the sheets required surgical placement; thus, they were suitable for surgically-managed periodontitis treatments. The adenovirus-mediated transfer of the HGF gene markedly decreased hDPSC apoptosis in a hypoxic environment or in serum-free medium, and it increased blood vessel regeneration.ConclusionThis study indicated that HGF-hDPSCs produced under GMP conditions significantly improved periodontal bone regeneration in swine; thus, this method represents a potential clinical application for periodontal regeneration.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-015-0244-5) contains supplementary material, which is available to authorized users.

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“…In periodontitis, the production of HGF is induced by various factors derived from the host, such as inflammatory cytokines and bacterial components. Studies have shown a correlation between the number of deep periodontal pockets and the salivary levels of HGF [ 31 , 32 ]. In the present study, a significantly higher level of salivary HGF was found in smokers with periodontitis and nonsmokers with periodontitis compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

Hepatocyte Growth Factor Levels in the Saliva and Gingival Crevicular Fluid in Smokers with Periodontitis

et al. 2014

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Hepatocyte growth factor (HGF) production by oral fibroblasts is enhanced by various molecules that are induced during inflammatory conditions including periodontitis. HGF plays an important role in the progression of periodontitis, by stimulating intense growth of epithelial cells and preventing regeneration of connective tissue attachments. Smokers have a greater risk factor in the pathogenesis and progression of periodontal disease. The objective of the study was to estimate the level of HGF in saliva and gingival crevicular fluid (GCF) in smokers with periodontitis and to compare these levels with that of nonsmokers with periodontitis and healthy controls. The HGF levels were found to be significantly high in the saliva and GCF of smokers with periodontitis compared to both never-smokers with periodontitis and the healthy control group. The elevated levels of HGF in the saliva and GCF in the study population could explain the intrinsic mechanism triggering the severity of the periodontitis in smokers. Further studies are necessary to validate the current observations and to establish a sensitive marker to predict periodontal disease activity.

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Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease

Cited by 15 publications

References 14 publications

Protein Biomarkers of Periodontitis in Saliva

Protein Biomarkers of Periodontitis in Saliva

Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine

Hepatocyte Growth Factor Levels in the Saliva and Gingival Crevicular Fluid in Smokers with Periodontitis

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