Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration

Dunn, James C.Y.; Yarmush, Martin L.; Koebe, H.G.; Tompkins, Ronald G.

doi:10.1096/fasebj.3.2.2914628

Cited by 705 publications

(517 citation statements)

References 19 publications

(1 reference statement)

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“…Hepatic tissue is formed of hepatocytes which are organized into a polarized epithelium with distinct apical and basal domains (Dunn et al 1989). Hepatocytes have a high metabolic activity and produce many liver specific molecules such as urea, amino acids, glycogen and bile.…”

Section: Livermentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

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To cite this version:Nicola C. Hunt, Liam M. Grover. Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnology Letters, Springer Verlag, 2010, 32 (6), pp.733-742. <10.1007/s10529-010-0221-0>. Abstract There has been a consistent increase in the mean life expectancy of the population of the developed world over the past century. Healthy life expectancy, however, has not increased concurrently. As a result we are living a larger proportion of our lives in poor health and there is a growing demand for the replacement of diseased and damaged tissues. While traditionally tissue grafts have functioned well for this purpose, the demand for tissue grafts now exceeds the supply. For this reason, research in regenerative medicine is rapidly expanding to cope with this new demand. There is now a trend towards supplying cells with a material in order to expedite the tissue healing process. Hydrogel encapsulation provides cells with a three dimensional environment similar to that experienced in vivo and therefore may allow the maintenance of normal cellular function in order to produce tissues similar to those found in the body. In this review we discuss biopolymeric gels that have been used for the encapsulation of mammalian cells for tissue engineering applications as well as a brief overview of cell encapsulation for therapeutic protein production. This review focuses on agarose, alginate, collagen, fibrin, hyaluronic acid and gelatin since they are widely used for cell encapsulation. The literature on the regeneration of cartilage, bone, ligament, tendon, skin, blood vessels and neural tissues using these materials has been summarised.

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Section: Livermentioning

confidence: 99%

Cell encapsulation using biopolymer gels for regenerative medicine

2010

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“…It has been suggested that the lack of dorsal interaction induces changes in cell morphology and cytoskeletal organization through a calcium signaling pathway [14]. In addition, it has been shown that cell phenotype, functionality and physiology can be drastically altered by exciting dorsal and ventral receptors [15][16][17][18]. So, although it can be argued that double-layer cultures might not provide a truly 3D environment, since cells are not isotropically stimulated, the use of this approach permits to engineer a large variety of experimental designs, with different material substrates and coating proteins, in a robust way (Fig.…”

Section: Introductionmentioning

confidence: 99%

Dorsal and Ventral Stimuli in Cell–Material Interactions: Effect on Cell Morphology

et al. 2012

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Cells behave differently between bidimensional (2D) and tridimensional (3D) environments. While most of the in vitro cultures are 2D, most of the in vivo extracellular matrices are 3D, which encourages the development of more relevant culture conditions, seeking to provide more physiological models for biomedicine (e.g., cancer, drug discovery and tissue engineering) and further insights into any dimension-dependent biological mechanism. In this study, cells were cultured between two protein coated surfaces (sandwich-like culture). Cells used both dorsal and ventral receptors to adhere and spread, undergoing morphological changes with respect to the 2D control. Combinations of fibronectin and bovine serum albumin on the dorsal and ventral sides led to different cell morphologies, which were quantified from bright field images by calculating the spreading area and circularity. Although the mechanism underlying these differences remains to be clarified, excitation of dorsal receptors by anchorage to extracellular proteins plays a key role on cell behavior. This approach-sandwich-like culture-becomes therefore a versatile method to study cell adhesion in well-defined conditions in a quasi 3D environment.

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“…Also, extracellular matrix components were shown to be important for the long-term (1 to 2 mo.) in vitro maintenance of hepatocyte form and function (Bissell et al, 1987;Dunn et al, 1989). Most recently, long-term culture (2 mo.)…”

mentioning

confidence: 99%

Colony isolation and secondary culture of fetal porcine hepatocytes on STO feeder cells

Talbot

Pursel

Rexroad

et al. 1994

In Vitro Cell Dev Biol - Animal

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SUMMARYThe secondary culture of non-transformed parenchymal hepatocytes has not been possible. STO feeder cell-dependent secondary cultures of fetal pig hepatocytes were established by colony isolation from primary cultures of 26-d fetal livers. The liver cells had the typical polygonal morphology of parenchymal hepatocytes. They also spontaneously differentiated to form small biliary canaliculi between individual cells or progressed further to large multicellular duct-like structures or cells undergoing gross lipid accumulation and secretion. The secondary hepatocyte cultures expressed alpha-fetoprotein (AFP), albumin, and f-fibrinogen mRNA, and conditioned medium from the cells contained elevated levels of transferrin and albumin. STO feeder cell co-culture may be useful for the sustainable culture of hepatocytes from other species.

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Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration

Cited by 705 publications

References 19 publications

Cell encapsulation using biopolymer gels for regenerative medicine

Cell encapsulation using biopolymer gels for regenerative medicine

Dorsal and Ventral Stimuli in Cell–Material Interactions: Effect on Cell Morphology

Colony isolation and secondary culture of fetal porcine hepatocytes on STO feeder cells

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