Hepatocyte culture in bioartificial livers with different membrane characteristics

Abstract

Rat hepatocytes were cultured in three polysulfone, hollow-fiber cartridges, characterized by two membrane variables: pore size and inner diameter (ID). Hepatocytes entrapped in a micro-filtration (MF) cartridge with the membrane pore size 0.1 microm had twice the production of urea and 4-fold the amount of albumin in comparison to the control cartridge, a ultra-filtration (UF) cartridge with a molecular weight cut-off (MWCO) of 100 kDa. Hepatocytes entrapped in a UF cartridge with ID of 0.5 mm secreted twice the amount of urea and 10-fold the amount of albumin compared with the control UF cartridge.

Keratinocyte serum-free medium maintains long-term liver gene expression and function in cultured rat hepatocytes by preventing the loss of liver-enriched transcription factors

“…The urea assay was based on a previously published protocol (Meng, Zhang, & Wu, 2004). Briefly, 100 μl of urea standards (0–50 μg/ml) and culture media collected over 24 h were incubated with 300 μl urease buffered solution (Sigma) at room temperature for 20 min.…”

Adipogenesis of Human Adipose-Derived Stem Cells Within Three-Dimensional Hollow Fiber-Based Bioreactors

“…There have been many reports on the use of hollow fiber bioreactor designs used for hepatocyte cultures. 14,[17][18][19][20][21] For example, Martin and Vermette have written a comprehensive review on the role of bioreactors for mass tissue culture.…”

A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond

“…A cell-suspension medium is passed through either the extra-or intra-capillary space to provide nutrients with cells seeded inside [54,55], or outside of the ibers [52,56]. HFBs have been successfully used [57][58][59][60][61], and in most experiments, 330 μm, 0.2 μm pore, and 150 μm wall thickness polypropylene hollow ibers are arranged along the axis of high-purity glass tubing, and were held in place with silicon rubber [54][55][56][57][58]. Potter and colleagues obtained a cartilaginous construct with one millimeter thickness after four weeks in an HFB [57].…”