Hepatocidol toxicity of Listeria species

Huang, Jianxiang; Huang, Hongyun; Jurima-Romet, Malle; Ashton, F. E.; Thomas, Barb R.

doi:10.1016/0378-1097(90)90312-e

Cited by 1 publication

(1 citation statement)

References 9 publications

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“…Nowadays, however, the availability of well-defined cell lines and standardized monolayers permits an evaluation of drug toxicity in vitro, thereby cutting down on the number of animal experiments (at least during the preliminary phases of investigation). Such in vitro models are already being employed in some medical fields (28)(29)(30)(31)(32)(33)(34) and have been suggested for pharmacologie testing of ocular drugs (35)(36)(37)(38)(39). For ethical (40), economical and political reasons, the use of such in vitro models is to be encouraged and recommended.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of Pentoxifylline on Monolayers of Highly Proliferative Cells of Epithelial Origin

Ventura

Böhnke²

1999

Journal of Ocular Pharmacology and Therapeutics

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Interest in pentoxifylline has been recently reawakened owing to its suppressive effect on cell cytokine production. In this capacity, it may be of value as a routine supplement for culture media containing donor corneas. The purpose of the present study was to evaluate the toxic effects of pentoxifylline on two standardized cell lines of epithelial origin. Vero and Chang cells were incubated with various concentrations of pentoxifylline. Acute toxicity (4 hr) was assessed by monitoring the permeability of cells to propidium iodide; chronic toxicity (7 days) was determined by monitoring the effect of pentoxifylline on esterase activity and cell proliferation. The viability of cells was also assessed by microscopic inspection. Signs of acute toxicity became manifest at a pentoxifylline concentration of 100 mg/l in both Chang and Vero cells. Indications of chronic toxicity were observed at a drug concentration of 10 mg/l in Chang cells but at 1 mg/l in Vero ones. Proliferation was suppressed at pentoxifylline concentrations of 100 mg/l and 10 mg/l in Chang and Vero cells, respectively. Degenerative morphological changes were observed at a drug concentration of 100 mg/l in both cell types. At a concentration of 0.1 mg/l, pentoxifylline elicited no signs of acute or chronic toxicity in either Chang or Vero cells. At this dose, the drug is therefore unlikely to have deleterious effects on cultured donor corneas.

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