“…We first studied the synergetic effect of hemin and sorafenib on SMMC7721 cells using cytotoxicity assessment with four different weight ratios of sorafenib/hemin (1:0.125, 1:0.25, 1:0.5, and 1:1), and analyzed the combination index values (CI). [ 24 ] Obviously, hemin alone was almost nontoxic to HCC cells. However, when combined with sorafenib, hemin considerably enhanced the cytotoxicity of sorafenib (Figure S1, Supporting Information), In addition, the CI values were 0.832, 0.776, 0.778, and 0.508, respectively, for the four different weight ratios of sorafenib/hemin (1:0.125, 1:0.25, 1:0.5, and 1:1), highlighting a synergetic effect of sorafenib and hemin combination (Table S1, Supporting Information).…”