Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles

Abstract

ORF3 86-88A mutant was duplicated and also shown as ORF3 95-97A mutant. In the corrected version of Fig. 5A shown below, we have replaced the duplicate image with the correct original data. After careful reexamination of the original data, we are confident that the error in assembling this figure does not affect the validity of the research or the conclusions that we have drawn. We are grateful to the careful reader who brought this mistake to our attention and apologize for any confusion that this error may have caused." The corrected Fig. 5 and its legend appear below.

The RNA genome of hepatitis E virus robustly triggers an antiviral interferon response

“…Plasmid constructs containing the full‐length HEV genome (Kernow‐C1 P6 clone) with 1634R or 1634K mutations were generated accordingly . Plasmid pLVX‐ORF2‐IRES‐zsGrenn1 was kindly provided by Alexander Ploss (Princeton University) . Plasmid pEGFP‐C1‐ORF3 was constructed in our lab.…”

Hepatitis E virus replication and interferon responses in human placental cells

“…Its genome contains a positive‐oriented single‐stranded RNA with three open reading frames (ORF), of which ORF1 encodes a multifunctional polyprotein involved in RNA replication, ORF2 the viral capsid protein, and ORF3 a nonstructural protein that can function as a viroporin 2, 3. HEV infections in humans are caused by five genotypes (gts) that differ in their worldwide distribution, their hosts, and their route of transmission.…”

Hepatitis E Virus Genotype 3 Genomes from RNA-Positive but Serologically Negative Plasma Donors Have CUG as the Start Codon for ORF3

“…At the N-terminal part of the protein two hydrophobic domains are located, D1 and D2, where D1 is important for cytoskeleton association [28]. Part of D1 and D2 act as an ion channel important for viral egress [29]. Two other regions, P1 and P2, are proline-rich domains.…”