Hepatitis C virus quantification in serum and saliva of HCV-infected patients

Menezes, Gisele Barreto Lopes; Pereira, Fernanda Albuquerque; Duarte, Cesar A. B.; Carmo, Theomira Mauadie Azevedo; Filho, Hermes Pedreira da Silva; Zarife, Maria Alice Sant'Anna; Krieger, Marco Aurélio; Reis, Eliana A. G.; Reis, Mitermayer Galvão dos

doi:10.1590/s0074-02762012000500016

Cited by 18 publications

(14 citation statements)

References 34 publications

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“…Other studies found that living with a family member infected with HCV [34,35] or suffering from liver disease [33,36,37] was an associated risk factor. Although it is known that multiple factors contribute to HCV transmissibility for saliva [5,38] and viral loads are very low in this [39]. The presence of HCV in saliva is to draw attention mainly in patients with active infection and high viral loads, patients who require guidance on their oral health by dentist.…”

Section: Discussionmentioning

confidence: 99%

Detection of hepatitis C virus RNA in saliva of patients with active infection not associated with periodontal or liver disease severity

et al. 2014

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BackgroundHepatitis C virus (HCV) is mainly transmitted by parenteral route, being blood transfusion and intravenous drug use the most frequent risk factors. However, it has been suggested that there are other routes of transmission. There are several studies where HCV RNA has been detected in saliva of patients infected with HCV, and epidemiological studies have proposed the dental treatments as possible risk factors for HCV transmission. The purpose of this study was to detect the presence of HCV RNA in saliva of patients with active infection and associating with periodontal or liver disease.MethodsPatients with quantifiable HCV-RNA in serum were enrolled in the study. Periodontal disease was assessed using the modified gingival index (MGI). Presence of dental plaque was assessed with the use of disclosing tablets. Patients were clinically and laboratory evaluated to identify the stage of liver disease, the HCV RNA was determinate in saliva by nested RT-PCR. To determine associations between different parameters univariate and multivariate analysis were used.ResultsA total of 45 patients were included. Of these patients, 21 (46.6%) had hepatitis, 23 (51.1%) had cirrhosis and one patient (2.4%) presented hepatocellular carcinoma (HCC). Viral loads in serum ranged from 2.31–6.68 log IU/ml with a mean of 5.46 log IU/ml (95% CI 5.23–5.70). HCV RNA was positive in saliva of 29 patients (64.4%) and was not detected in 16 (35.6%). For univariate analysis three independent variables were associated with the detection of HCV-RNA in saliva: gender, viral load and dental plaque and multivariate analysis only one independent variable viral load >5.17 log IU/mL remained significantly associated with the detection of HCV in saliva (p = 0.0002). A statistical difference was observed when viral load was analyzed, log 5.85 IU/mL (95% CI 5.67–6.02) for patients with HCV in saliva vs. log 4.77 IU/mL (95% CI 4.35–5.19) for patients without HCV in saliva (p = 0.0001). The detection of HCV-RNA in saliva was more frequent in patients with relatively high serum viral loads.ConclusionHCV-RNA in saliva was associated with the level of serum viral load but not with periodontal or liver disease severity.

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Section: Discussionmentioning

confidence: 99%

Detection of hepatitis C virus RNA in saliva of patients with active infection not associated with periodontal or liver disease severity

et al. 2014

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“…The literature (2)(3)(4)(5) refers to the presence of RNA-HCV in body fluids such as saliva, peripheral lymph nodes and semen. It is worth noting that although the virus is found in low titrations, this form of transmission must be considered.…”

Section: Introductionmentioning

confidence: 99%

“…There are innumerable studies on the presence of RNA-HCV in the saliva (2)(3)(4)(5) but their results vary, reflecting the heterogeneity of the populations studied, and the variety of detection techniques used. Nevertheless, epidemiological studies suggest that the infectious capacity of viral particles of HCV in the saliva is low.…”

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confidence: 99%

Seroprevalence of Hepatitis C and factors associated with this in crack users

Sá

Araújo

Griep

et al. 2013

Rev. Latino-Am. Enfermagem

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OBJECTIVE: to investigate the seroprevalence of hepatitis C in crack users in Piauí. METHOD: seroepidemiological survey, undertaken in the Psycho-Social Care Centers for Drugs and Alcohol (CAPS AD) in Piauí in the period December 2011 to May 2012. A questionnaire was administered and blood samples were collected for serological research. Fisher's exact test and the Mann-Whitney test were used, with a level of significance of (p<0.05). RESULTS: the prevalence of Anti-HCV was 05 (1.4%) and 04 (1.1%) for the RNA-HCV. There was a statistically significant association between hepatitis C (serological marker RNA-HCV) and age, being resident at home, length of use of crack, interruption of the use of crack, and the habit of sharing the crack pipes. CONCLUSION: the findings support the need to implement health policies aimed at crack users, due to the accelerated process of physical and psychological deterioration to which these are subject.

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“…Some authors have the HCV as a virus with a triple tropism (Hepatotropism, lymphotropism, and sialotropism), which could explain the xerostomia in these patients [14]. In addition, the reduced salivary flow rate (hyposalivation) in Hepatitis C patients could be due to infiltration of the salivary gland by the virus [12] or to a possible virus induced immune mechanism [22,23]. However, in only 3 cases (12.5%) xerostomia and hyposalivation were observed, simultaneosly.…”

Section: Discussionmentioning

confidence: 99%

Stimulated And Non-Stimulated Salivary Flows Should Be Tested For The Presence Of HCV RNA In Saliva Samples From Patients With Chronic Hepatitis C

Smc¹,

Oliveira²,

Teixeira³

et al. 2014

Dentistry

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Objective: In most of the studies which analyzed the presence of HCV RNA in saliva from patients with chronic Hepatitis C only stimulated saliva samples have been used for viral detection. Thus, this study compared the prevalence of HCV RNA in non-stimulated and stimulated salivary flows in patients with chronic Hepatitis C.Design: Saliva samples of non-stimulated and stimulated salivary flows from 24 patients were collected, and the HCV RNA was investigated by RT-nested PCR. Data regarding age, gender, risk factors for HCV infection, xerostomia and hyposalivation were also analyzed. Results:The HCV RNA could be detected in 11 (45.8%) non-stimulated and in 14 (58.3%) stimulated saliva samples, without statistical significance (p=0.472). However, in 18 (75.0%) patients it was possible to detect the presence of the HCV RNA at least in one of the saliva samples. Six (25.0%) patients complained of xerostomia and nine (37.5%) presented hyposalivation, but in only 3 (12.5%) patients, these conditions could be observed, simultaneously. No significant correlation between the presence of HCV RNA in saliva and age, gender, risk factors for HCV infection, xerostomia and hyposalivation could be identified. Conclusion:Both stimulated and non-stimulated saliva samples must be investigated for the presence of HCV RNA in patients with chronic Hepatitis C, to avoid underestimated prevalence of HCV in this group of patients.

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Hepatitis C virus quantification in serum and saliva of HCV-infected patients

Abstract: The hepatitis C virus (HCV)

Cited by 18 publications

References 34 publications

Detection of hepatitis C virus RNA in saliva of patients with active infection not associated with periodontal or liver disease severity

Detection of hepatitis C virus RNA in saliva of patients with active infection not associated with periodontal or liver disease severity

Seroprevalence of Hepatitis C and factors associated with this in crack users

Stimulated And Non-Stimulated Salivary Flows Should Be Tested For The Presence Of HCV RNA In Saliva Samples From Patients With Chronic Hepatitis C

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