Hepatitis C virus plus‐ and minus‐ strand RNA in hepatocellular carcinoma and adjoining nontumorous liver

Horiike, Norio; Nonaka, Takashi; Kumamoto, Izumi; Kajino, Kazunori; Onji, Morikazu; Ohta, Yasuyuki

doi:10.1002/jmv.1890410410

Cited by 24 publications

(15 citation statements)

References 15 publications

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“…To examine the relationship between CYP 3A4 mRNA in blood and liver, blood and liver samples (1 mg of frozen liver tissue) were collected simultaneously in 7 patients with CH and 3 patients with LC. RNA was extracted as previously reported [7]. CYP 3A4 mRNA was quantified using the competitive reverse transcription polymerase chain reaction (CRT-PCR) as reported [8].…”

Section: Assay Of Cyp450 3a4 Mrna In Blood or Livermentioning

confidence: 99%

The quantification of cytochrome P-450 (CYP 3A4) mRNA in the blood of patients with viral liver diseases

Horiike

Abe

Kumagi

et al. 2005

Clinical Biochemistry

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Section: Assay Of Cyp450 3a4 Mrna In Blood or Livermentioning

confidence: 99%

The quantification of cytochrome P-450 (CYP 3A4) mRNA in the blood of patients with viral liver diseases

Horiike

Abe

Kumagi

et al. 2005

Clinical Biochemistry

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“…HCV may produce in normal and transformed liver cells, as evidenced by both positive-and negativestranded HCV-RNA [Gerber et al, 1992;Takeda et al, 1992;Gerber, 1993;Horiike et al, 1993;Kobayashi et al, 1994;Kurosaki et al, 1995;Niu et al, 1995] as well as HCV proteins in HCC tissue [Haruna et al, 1994;Uchida and Shikata, 1994;Sansonno et al, 1997]. The literature contains only limited information regarding absolute and relative HCV load levels in normal and tumor liver tissue [Park et al, 1997;Dash et al, 2000].…”

Section: Introductionmentioning

confidence: 98%

Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non‐cancerous liver

Young¹,

Lin²,

Hsiao³

et al. 2002

Journal of Medical Virology

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Hepatitis C virus (HCV) infection is etiologically associated with the development of hepatocellular carcinoma (HCC) worldwide. HCV has been reported to exist and replicate in both HCC and adjacent non-cancerous liver tissue, but limited information was available on HCV viral load and quasispecies composition in HCC relative to adjacent non-cancerous hepatocytes. Previous study has also suggested CD81, a surface hepatocyte protein, as a receptor for HCV. To clarify the above, HCV-RNA and CD81-RNA titers in 20 paired hepatectomized liver and serum were quantitatively measured by chemiluminescent RT-cPCR. Hypervariable region 1 (HVR-1) variations of parallel specimens were analyzed after subcloning in 6 patients. HCV-RNA levels in serum and non-cancerous liver were markedly higher for HCV genotype 1 than genotype non-1. HCV levels were markedly higher in non-cancerous liver than in HCC (P = 0.001) in a genotype-independent manner, with a mean ratio of 56:1 for non-cancerous tissue to HCC. Both non-cancerous and HCC tissues had the same level of CD81-RNA expression, which was not linked to HCV load. HCV-RNA quantity in both HCC and non-cancerous liver correlated with the number of HVR-1 quasispecies in the tissue, and distinct HVR-1 subclones existed.

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“…Using PCR, the presence of HCV-RNA can be confirmed in samples with positive or indeterminate EIA results. Hepatitis C viremia is known to be present in most patients with chronic hepatitis C, including those with a long-standing infection or advanced liver disease such as cirrhosis or hepatocellular carcinoma 10 . Most (80%-90%) EIA-2-positive patients with chronic hepatitis C are RIBA-2 and HCV-RNA positive while approximately 10% are RIBA indeterminate and 60%-70% of whom will be HCV-RNA positive 2,14 .…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay

Gonçales

Costa

Vassallo

et al. 2000

Rev. Inst. Med. trop. S. Paulo

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SUMMARYScreening blood donations for anti-HCV antibodies and alanine aminotransferase (ALT) serum levels generally prevents the transmission of hepatitis C virus (HCV) by transfusion. The aim of the present study was to evaluate the efficiency of the enzyme immunoassay (EIA) screening policy in identifying potentially infectious blood donors capable to transmit hepatitis C through blood transfusion. We have used a reverse transcriptase (RT)-nested polymerase chain reaction (PCR) to investigate the presence of HCV-RNA in blood donors. The prevalence of HCV-RNA positive individuals was compared with the recombinant immunoblot assay (RIBA-2) results in order to assess the usefulness of both tests as confirmatory assays. Both tests results were also compared with the EIA-2 OD/C ratio (optical densities of the samples divided by the cut off value). ALT results were expressed as the ALT quotient (qALT), calculated dividing the ALT value of the samples by the maximum normal value (53UI/l) for the method. Donors (n=178) were divided into five groups according to their EIA anti-HCV status and qALT: group A (EIA³3, ALT<1), group B (EIA³3, ALT>1), group C (1£EIA<3, ALT<1), group D (1£EIA<3, ALT>1) and group E (EIA£0.7). HCV sequences were detected by RT-nested PCR, using primers for the most conserved region of viral genome. RIBA-2 was applied to the same samples. In group A (n=6), all samples were positive by RT-nested PCR and RIBA-2. Among 124 samples in group B, 120 (96.8%) were RIBA-2 positive and 4 (3.2%) were RIBA-2 indeterminate but were seropositive for antigen c22.3. In group B, 109 (87.9%) of the RIBA-2 positive samples were also RTnested PCR positive, as well as were all RIBA-2 indeterminate samples. In group C, all samples (n=9) were RT-nested PCR negative: 4 (44.4%) were also RIBA-2 negative, 4 (44.4%) were RIBA-2 positive and 1 (11.1%) was RIBA-2 indeterminate. HCV-RNA was detected by RT-nested PCR in 3 (37.5%) out of 8 samples in group D. Only one of them was also RIBA-2 positive, all the others were RIBA-2 indeterminate. All of the group E samples (controls) were RT-nested PCR and RIBA-2 negative. Our study suggests a strong relation between anti-HCV EIA-2 ratio ³ 3 and detectable HCV-RNA by RT-nested PCR. We have also noted that blood donors with RIBA-2 indeterminate presented a high degree of detectable HCV-RNA using RT-nested PCR (75%), especially when the c22.3 band was detected.

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Hepatitis C virus plus‐ and minus‐ strand RNA in hepatocellular carcinoma and adjoining nontumorous liver

Cited by 24 publications

References 15 publications

The quantification of cytochrome P-450 (CYP 3A4) mRNA in the blood of patients with viral liver diseases

The quantification of cytochrome P-450 (CYP 3A4) mRNA in the blood of patients with viral liver diseases

Variation of hepatitis C virus load, hypervariable region 1 quasispecies and CD81 hepatocyte expression in hepatocellular carcinoma and adjacent non‐cancerous liver

Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay

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