Hepatitis C virus genotypes in a northeastern area of Brazil.

Silva, L K; Paraná, Raymundo; Souza, Samilly Evangelista; Berby, Françoise; Kay, Alan; Trépo, C.; Santana, Nelma; Cotrim, Helma Pinchemel; Lyra, Luiz Guilherme Costa; Reis, Mitermayer Galvão dos

doi:10.4269/ajtmh.2000.62.257

Cited by 36 publications

(35 citation statements)

References 35 publications

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“…e HCV-RNA percentage in HCV antibody-positive patients in our community was 61.1% (124/203). Similar disparities in ratio between HCV antibody-positive and HCV-RNA viral load detectable plasma levels have been reported by others (10,11).…”

Section: Discussionsupporting

confidence: 85%

Most common HCV genotypes in patients from north-eastern Croatia

Perić

Bošnjak

Džijan

et al. 2014

AMA

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Objective. e aims of this study were to determine the HCV-RNA viral load, genotype distribution, risk factors and symptoms of HCV-RNA positive viral load in HCV antibody-positive patients from north-eastern Croatia. Materials and methods. From January 2009 to December 2011, 203 HCV antibody-positive patients (130 men and 73 women; median age 44.5 years) were analyzed for HCV-RNA by the COBAS TaqMan HCV test and genotyped by the Linear Array HCV Genotyping test (both from Roche). All patients completed a structured questionnaire about risk factors and symptoms. Results.e HCV-RNA percentage was 61.1% and was similar for men and women. e HCV-RNA viral load increased with age: while 55% of 20-50 year old patients were HCV-RNA positive, 73% of patients >50 years were positive (p=0.021). Genotype 1 was the most prevalent genotype (79.8%), followed by 3 (12.9%), 4 (6.5%), and 2 (0.8%); genotypes 5 and 6 were not determined. Patients with genotype 1 (median, 50 years) were older than patients with 3 (median, 33.5 years) or 4 (median, 38 years). e blood transfusions performed in Croatian hospitals before 1993 was signi cantly associated with HCV-RNA positive viral load (p<0.05). Conclusion.ese data indicated an elevated prevalence of genotype 1 in elderly HCV-RNA positive patients and it may continue to rise. Using RNA-based detection in HCV positive-antibody patients would allow early detection of HCV in the acute stage of HCV disease and the increased risk of HCV genotyperelated treatment failure.

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Section: Discussionsupporting

confidence: 85%

Most common HCV genotypes in patients from north-eastern Croatia

Perić

Bošnjak

Džijan

et al. 2014

AMA

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“…In our study, the HCV-RNA frequency in plasma samples from anti-HCV-positive patients (82.5%) was similar to that reported in other studies in Brazil [42,44]. The predominance of genotype 1 (68.75% of all HCV-RNA positive cases) also concurs with published data from other studies conducted in Brazil [42,[44][45][46].…”

Section: Discussionsupporting

confidence: 91%

“…The predominance of genotype 1 (68.75% of all HCV-RNA positive cases) also concurs with published data from other studies conducted in Brazil [42,[44][45][46].…”

Section: Discussionsupporting

confidence: 90%

Detection of hepatitis C virus RNA in saliva samples from patients with seric anti-HCV antibodies

Gonçalves¹,

Cunha²,

Busek³

et al. 2005

Braz J Infect Dis

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We examined the frequency of HCV-RNA in saliva samples from anti-HCV positive patients. Both plasma and saliva samples from 39 HCV patients (13 with normal liver enzymes, 19 with abnormal liver enzymes and 13 with cirrhosis) were investigated. Stimulated saliva and fresh plasma were centrifuged (900 x g,10 min) and stored at -70 o C, after the addition of guanidine isothiocyanate RNA extraction buffer. HCV-RNA was detected by RT-nested-PCR (amplification of HCV-cDNA for two rounds, using HCV primers 939/209 and 940/211). HCV genotyping was carried out by RFLP (using Mva I and Hinf 1 or Hae III and Rsa I restriction enzymes). Thirty-two out of 39 (82%; 95% CI=70-94%) anti-HCV-positive patients had HCV-RNA in plasma samples. Eight out of 39 (20.5%; 95% CI=6.6-34.4%) had HCV-RNA in the saliva. The HCV genotype in saliva samples from these patients matched the genotype found for plasma HCV-RNA. No significant correlation between the presence of HCV and either age, gender, HCV genotype or any risk factor for HCV infection was found. The observed prevalence (20.5% of anti HCV positive patients or 25% of the patients with HCV-RNA in plasma) was lower than that previously reported from other countries. The low frequency of HCV-RNA in saliva samples observed in our study may be due to the use of cell-free saliva. Other authors reporting higher frequencies of HCV-RNA in saliva used whole saliva, without centrifugation.

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“…Among the Northeastern states, published data are available only for Rio Grande do Norte (21), Ceará (23), and Bahia (27). We analyzed a small number of samples from these states and obtained comparable results.…”

Section: Discussionmentioning

confidence: 85%

Geographic distribution of hepatitis C virus genotypes in Brazil

Campiotto

Pinho

Carrilho

et al. 2005

Braz J Med Biol Res

174

102

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Brazil is a country of continental dimension with a population of different ethnic backgrounds. Thus, a wide variation in the frequencies of hepatitis C virus (HCV) genotypes is expected to occur. To address this point, 1,688 sequential samples from chronic HCV patients were analyzed. HCV-RNA was amplified by the RT-PCR from blood samples collected from 1995 to 2000 at different laboratories located in different cities from all Brazilian States. Samples were collected in tubes containing a gel separator, centrifuged in the site of collection and sent by express mail in a refrigerated container to Laboratório Bioquímico Jardim Paulista, São Paulo, SP, Brazil. HCV-RNA was extracted from serum and submitted to RT and nested PCR using standard procedures. Nested PCR products were submitted to cycle sequencing reactions without prior purification. Sequences were analyzed for genotype determination and the following frequencies were found: 64.9% (1,095) for genotype 1, 4.6% (78) for genotype 2, 30.2% (510) for genotype 3, 0.2% (3) for genotype 4, and 0.1% (2) for genotype 5. The frequencies of HCV genotypes were statistically different among Brazilian regions (P = 0.00017). In all regions, genotype 1 was the most frequent (51.7 to 74.1%), reaching the highest value in the North; genotype 2 was more prevalent in the Center-West region (11.4%), especially in Mato Grosso State (25.8%), while genotype 3 was more common in the South (43.2%). Genotypes 4 and 5 were rarely found and only in the Southeast, in São Paulo State. The present data indicate the need for careful epidemiological surveys throughout Brazil since knowing the frequency and distribution of the genotypes would provide key information for understanding the spread of HCV.

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Hepatitis C virus genotypes in a northeastern area of Brazil.

Cited by 36 publications

References 35 publications

Most common HCV genotypes in patients from north-eastern Croatia

Most common HCV genotypes in patients from north-eastern Croatia

Detection of hepatitis C virus RNA in saliva samples from patients with seric anti-HCV antibodies

Geographic distribution of hepatitis C virus genotypes in Brazil

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