Hepatitis C: Exploration of Diseases, Diagnosis, and Treatment Strategies

Masood, Laiba; Islam, Nayyer; Irfan, Muhammad; Zahoor, Ameer Fawad; Syed, Haroon Khalid; Shah, Muhammad Shahid; Shah, Muhammad Ajmal; Syed, Muhammad Ali; Hanif, Sana

doi:10.1007/978-3-031-20521-7_15

Cited by 1 publication

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nucleic acid amplification (NAT) is the ‘gold standard’ for the detection of active HCV replication 40 . It is exceptionally useful for the diagnosis of acute HCV infection, because of the detection of RNA as early as 1 week after exposure via needle-stick or blood transfusion, and at least 4–6 weeks prior to seroconversion 41 . Overall, the detection of HCV by qualitative RNA test is among the extremely sensitive tests used presently.…”

Section: Discussionmentioning

confidence: 99%

Determination of hepatitis C virus subtype prevalent in Sindh, Pakistan: a phylogenetic analysis

Farooq,

Faiz,

Wahab

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Hepatitis is a major public health issue, affecting 10–17 million people worldwide, with its prevalence continuously increasing. The Hepatitis C virus (HCV) is responsible for liver related diseases, which include liver cirrhosis, hepatocellular carcinoma, and chronic hepatitis. Pakistan is experiencing a serious rise in HCV cases. This study aimed to assess the prevalence and distribution of HCV genotypes in Sindh, Pakistan. Serum samples from HCV-positive patients were collected from various local hospitals in Sindh. These samples were first screened for HCV antibodies using ELISA. Samples that tested positive for HCV RNA underwent further genotyping through sequencing using the standard Sanger method. The genotypes were identified by comparing the sequences with those available in the National Center for Biotechnology Information (NCBI) database, and a phylogenetic tree was constructed. The phylogenetic analysis showed that all isolates in this study were clustered with genotypes 3a and 3b, except for one sequence that was clustered with genotype 1a. No isolates were found to be clustered with reference genomes of genotypes 2, 4, 5, 6, and 7 suggesting that genotype 3a is endemic in this region. The analyzed sequences demonstrated a 98% similarity with reference and isolated sequences. In summary, sequencing of the HCV 5′ UTR essential for identifying the predominant genotype of HCV RNA in the Sindh region Further research on the distribution of HCV genotypes in other regions of Pakistan could aid in improving screening processes, identifying more effective treatment options, and developing suitable prevention strategies.

show abstract

Section: Discussionmentioning

confidence: 99%