Hepatitis "C" Antigen in Non-A, Non-B Post-Transfusion Hepatitis

Shirachi, Ryoichi; Shiraishi, Hiroaki; Tateda, Akira; Kikuchi, Kumiko; Ishida, Nakao

doi:10.1016/s0140-6736(78)91567-2

Cited by 117 publications

(30 citation statements)

References 7 publications

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“…The prevalence of a non-A, non-B hepatitis virus carrier state among blood donors may, perhaps, be even higher than data of post-transfusion hepatititis would suggest, since at least some recipients of blood would be expected to have antibodies to the corresponding virus(es). Shirachi et al (1978) recently detected a new antigen (designated hepatitis C) by immunodiffusion in acute-phase sera of 17 out of 23 patients with post-transfusion hepatitis type non-A, non-B. This antigen appears to have properties other than HRA described here.…”

Section: Discussionmentioning

confidence: 59%

An Antigen Detected Frequently in Human Sera With Elevated Levels of Alanine Aminotransferase: A Potential Marker For Non-A, Non-B Hepatitis

Neurath

Stevens

Strick

et al. 1980

Journal of General Virology

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SUMMARYIn a search for additional antigens associated with virus-induced human liver disease a radioimmunoassay (RIA) was developed using IgG from sera of a multiply transfused person. Polystyrene beads coated with IgG F(ab); fragments, F ' dinitrophenylated (ab)2 fragments and a~SI-labelled anti-z,4-dinitrophenyl antibodies (Neurath & Strick, I979) were used in the RIA. An apparently new antigen or the corresponding antibodies were detected in t55 serum specimens from 35/37 (94 %) individuals who developed non-A, non-B hepatitis. The antigen was also present in hepatitis B surface antigen-negative sera of blood donors with normal (13"1%) and elevated levels ofalanine aminotransferase (34 %). The antigen has an approximate mol. wt. of 45ooo, a buoyant density of 1.23 g/ml and an isoelectric point of 7.

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Section: Discussionmentioning

confidence: 59%

An Antigen Detected Frequently in Human Sera With Elevated Levels of Alanine Aminotransferase: A Potential Marker For Non-A, Non-B Hepatitis

Neurath

Stevens

Strick

et al. 1980

Journal of General Virology

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“…Interpretation of the findings in patients with non-A, non-B hepatitis is complicated by the absence of viral markers, making it difficult to determine the stage of the illness in relation to viral replication and liver damage. A further complication is the recognition of two main subgroups differing in their incubation periods,12 13 and until serological tests become available, it will not be 4-10 >10 possible to determine whether the heterogeneity of the anti-LSP response reflects differences in the cetamol-induced immunopathology of these two varieties of the P titre in serum disease, variations in the immune response of gtransferase different individuals or in the time of presentation in resentation.…”

Section: Resultsmentioning

confidence: 99%

Anti-LSP antibodies in acute liver disease.

Melicòni

Perperas

Alberti³

et al. 1982

Gut

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SUMMARY Sera from 71 patients with acute liver injury have been tested for antibodies to hepatocyte membrane lipoprotein complex (LSP) using a sensitive radioimmunoassay. Two main patterns of anti-LSP response were seen. In the first, seen in patients with type A and B viral hepatitis, anti-LSP antibodies were detectable at presentation, with the highest titres two to 10 days before the peak in serum aminotransferases and, in the hepatitis B patients, when viral DNA polymerase concentrations were still high, indicating active viral replication. These findings are consistent with the anti-LSP response being consequent on an interaction between T cells and neoantigens on the liver cell surface. A similar pattern was found in halothane hepatitis where immune responses to a halothane-altered liver membrane antigen are present early in the course of the disease. In the second type of response, exemplified by cases with paracetamol-induced hepatic necrosis, anti-LSP was only occasionally detectable at presentation, although present in very low titre later in the clinical course. This may be due to the release of altered antigen at the time of hepatocellular injury. The same pattern was found in a selected group of patients with uncomplicated acute alcoholic hepatitis, suggesting that in both these groups of patients the liver damage may have been due to a direct toxic effect on liver cells.

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“…Before the isolation of the virus by molecular biological techniques [2], many antigens were claimed to be associated with hepatitis C (non-A, non-B hepatitis; NANBH) using immunological techniques [3][4][5]. We found an antigen (AN6520 antigen; AN-Ag) in the liver of patients with NANBH which formed a precipitin line with convalescent sera from patients with NANBH.…”

Section: Introductionmentioning

confidence: 88%

Autoantibody response to microsomal epoxide hydrolase in hepatitis C and A

Akatsuka

Kobayashi

Ishikawa

et al. 2007

Journal of Autoimmunity

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Autoimmune responses were observed in a large proportion of hepatitis C cases and are suspected to be part of viral pathogenesis. The AN6520 antigen (AN-Ag) is a normal cellular protein mainly expressed in liver that was found associated with non-A, non-B hepatitis. To elucidate its pathogenic role in hepatitis C, we developed an IgM capture assay using purified AN-Ag and confirmed that the antibody response to AN-Ag is associated almost exclusively with hepatitis C cases (29%). Screening of a human liver expression library revealed that AN-Ag is mainly the microsomal epoxide hydrolase (mEH), a drug-metabolizing enzyme that plays an important role in the metabolism of some mutagenic and carcinogenic epoxides. Using the purified recombinant human mEH as an antigen, we now found that antibodies against this protein are associated with nearly 82% of hepatitis C virus infections and surprisingly with 46% of patients with hepatitis A. The appearance of AN-Ag/mEH in the incubation period of hepatitis C as previously reported and the antibody responses shown here indicate that this enzyme may be a marker for or even a cause of some of the pathology associated with hepatitis C and A.

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Hepatitis "C" Antigen in Non-A, Non-B Post-Transfusion Hepatitis

Cited by 117 publications

References 7 publications

An Antigen Detected Frequently in Human Sera With Elevated Levels of Alanine Aminotransferase: A Potential Marker For Non-A, Non-B Hepatitis

An Antigen Detected Frequently in Human Sera With Elevated Levels of Alanine Aminotransferase: A Potential Marker For Non-A, Non-B Hepatitis

Anti-LSP antibodies in acute liver disease.

Autoantibody response to microsomal epoxide hydrolase in hepatitis C and A

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