Hepatitis B virus surface protein induces oxidative stress by increasing peroxides and inhibiting antioxidant defences in human spermatozoa

Cheng, Lin; Sun, Pingnan; Xie, Xiaoling; Sun, Donglin; Zhou, Qi; Yang, Shaozhe; Xie, Qiang; Zhou, Xiaoling

doi:10.1071/rd20130

Cited by 8 publications

(8 citation statements)

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“…To our knowledge, no previous studies have evaluated the interaction effect of both spouses’ HBV infection prior to pregnancy on risk of CHDs in offspring. HBV infection has been reported to affect not only oocytes but also sperm, and there may be an interaction effect of spouses’ preconception HBV infection statuses . In this study, increased CHD risks were observed only in women previously infected with HBV with uninfected men or previously infected men with uninfected women prior to pregnancy.…”

Section: Discussionmentioning

confidence: 53%

“…HBV infection has been reported to affect not only oocytes but also sperm, and there may be an interaction effect of spouses' preconception HBV infection statuses. [27][28][29][30][31][32][33] In this study, increased CHD risks were observed only in women previously infected with HBV with uninfected men or previously infected men with uninfected women prior to pregnancy. Moreover, previously infected men with uninfected women had a higher prevalence of CHDs in offspring than previously infected women with uninfected men.…”

Section: Discussionmentioning

confidence: 97%

“…[19][20][21][22][23][24] Although the most recent study has reported that maternal HBV positivity is related to an increased risk of CHDs in offspring in early pregnancy, 16 there is little evidence about the association of maternal preconception HBV infection with CHD risk. Given the possible mechanisms for HBV prenatal transmission, such as placental infection and transplacental transmission of HBV 25,26 or HBV-DNA existing in infected maternal oocytes or paternal sperms, [27][28][29][30][31][32][33] there might be a potential correlation between maternal preconception HBV infection and CHDs in offspring.…”

mentioning

confidence: 99%

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Maternal Preconception Hepatitis B Virus Infection and Risk of Congenital Heart Diseases in Offspring Among Chinese Women Aged 20 to 49 Years

Yang

Jia

et al. 2023

JAMA Pediatr

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ImportanceMaternal hepatitis B virus (HBV) infection during early pregnancy has been related to congenital heart diseases (CHDs) in offspring. However, no study to date has evaluated the association of maternal preconception HBV infection with CHDs in offspring.ObjectiveTo explore the association of maternal preconception HBV infection with CHDs in offspring.Design, Setting, and ParticipantsThis retrospective cohort study used nearest-neighbor (1:4) propensity score matching of 2013 to 2019 data from the National Free Preconception Checkup Project (NFPCP), a national free health service for childbearing-aged women who plan to conceive throughout mainland China. Women aged 20 to 49 years who got pregnant within 1 year after preconception examination were included, and those with multiple births were excluded. Data were analyzed from September to December 2022.ExposuresMaternal preconception HBV infection statuses, including uninfected, previous, and new infection.Main Outcomes and MeasuresThe main outcome was CHDs, which were prospectively collected from the birth defect registration card of the NFPCP. Logistic regression with robust error variances was used to estimate the association between maternal preconception HBV infection status and CHD risk in offspring, after adjusting for confounding variables.ResultsAfter matching with a 1:4 ratio, there were 3 690 427 participants included in the final analysis, where 738 945 women were infected with HBV, including 393 332 women with previous infection and 345 613 women with new infection. Approximately 0.03% (800 of 2 951 482) of women uninfected with HBV preconception and women newly infected with HBV carried an infant with CHDs, whereas 0.04% (141 of 393 332) of women with HBV infection prior to pregnancy carried an infant with CHDs. After multivariable adjustment, women with HBV infection prior to pregnancy had a higher risk of CHDs in offspring compared with women who were uninfected (adjusted relative risk ratio [aRR], 1.23; 95% CI, 1.02-1.49). Moreover, compared with couples who were uninfected with HBV prior to pregnancy (680 of 2 610 968 [0.026%]), previously infected women with uninfected men (93 of 252 919 [0.037%]) or previously infected men with uninfected women (43 of 95 735 [0.045%]) had a higher incidence of CHDs in offspring and were significantly associated with a higher risk of CHDs in offspring (previously infected women with uninfected men: aRR, 1.36; 95% CI, 1.09-1.69; previously infected men with uninfected women: aRR, 1.51; 95% CI, 1.09-2.09) with multivariable adjustment, while no significant association was observed between maternal new HBV infection and CHDs in offspring.Conclusions and RelevanceIn this matched retrospective cohort study, maternal preconception previous HBV infection was significantly associated with CHDs in offspring. Moreover, among women with HBV-uninfected husbands, significantly increased risk of CHDs was also observed in previously infected women prior to pregnancy. Consequently, HBV screening and getting HBV vaccination-induced immunity for couples prior to pregnancy are indispensable, and those with previous HBV infection prior to pregnancy should also be taken seriously to decrease the CHDs risk in offspring.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 97%

mentioning

confidence: 99%

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Maternal Preconception Hepatitis B Virus Infection and Risk of Congenital Heart Diseases in Offspring Among Chinese Women Aged 20 to 49 Years

Yang

Jia

et al. 2023

JAMA Pediatr

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“…It destroys various body tissues and causes a variety of diseases, including diabetes, hepatitis, and neurodegenerative diseases. 85 Many viruses induce oxidative stress; such as respiratory syncytial virus (RSV), 86 EBV, 87 Pseudorabies virus (PRV), 88 Canine distemper virus (CDV), 89 Hepatitis B virus (HBV), 90 Mayaro virus (MAYV), 91 SARS-CoV-2, 92 Hepatitis C virus (HCV), 93 Human immunodeficiency virus(HIV), 94 Human papillomavirus (HPV), 95 Rotavirus, 96 Rabies virus, 97 Rubella virus (RV), 98 HSV, 99 ZIKV, 100 Japanese encephalitis virus (JEV), West Nile virus (WNV), Tick-borne encephalitis virus (TBEV), 101 Dengue virus (DENV), 102 Chikungunya virus, 103 Nipah virus (NiV), 104 Bovine leukemia virus (BLV), 105 Influenza a virus, 106 Chandipura virus (CHPV), 107 Newcastle disease virus (NDV), 108 African swine fever virus (ASFV), 109 Grass carp reovirus (GCRV), 110 and E. huxleyi virus (EHV). 111 Melissa has been proven to have anti-oxidative properties 50 and applies one of its antiviral properties through the same property.…”

Section: Oxidative Stressmentioning

confidence: 99%

Antiviral Potential of Melissa officinalis L.: A Literature Review

Behzadi

imani

Deravi

et al. 2023

Nutr Metab Insights

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The use of synthetic drugs has increased in recent years; however, herbal medicine is yet more trusted among a huge population worldwide; This could be due to minimal side effects, affordable prices, and traditional beliefs. Lemongrass ( Melissa officinalis) has been widely used for reducing stress and anxiety, increasing appetite and sleep, reducing pain, healing wounds, and treating poisonous insect bites and bee stings for a long time. Today, research has shown that this plant can also fight viruses including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Herpes Simplex Virus (HSV), and Human Immunodeficiency Virus (HIV) through various mechanisms such as inhibiting HSV-1 from binding to host cell, inhibiting HSV-1 replication during the post-adsorption or inhibiting main protease and spike protein of SARS-CoV-2, furthermore, be effective in treating related diseases. This Review investigated the antiviral properties of Melissa officinalis and its effect on viral diseases. More in vitro and in vivo studies are needed to determine Melissa officinaliss underlying mechanism, and more randomized controlled trials should be done to identify its effect in humans. Also, due to the usefulness and lack of side effects, it can be used more as a complementary medicine.

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“…HBV surface proteins (HBs) exposure of sperm cell could cause sperm dysfunction and sperm death because of reactive oxygen species (ROS) generation and alteration of glutathione (GSH) peroxidase 4 (GPX4) activity. [17][18][19][20] GPX4 is currently regarded as the molecular marker and key regulator of ferroptosis. 21 Oxidative stress and GPX4 dysfunction are essential for ferroptosis.…”

Section: Introductionmentioning

confidence: 99%

The role of hepatitis B virus surface protein in inducing Sertoli cell ferroptosis

Pan,

Kong,

et al. 2023

Andrology

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BackgroundsHepatitis B virus infection could result in male infertility with sperm defects and dysfunction. Sertoli cells are essential for testis function and play a crucial role in spermatogenesis. Sertoli cell death contributes to spermatogenesis impairment, leading to poor sperm quality. Ferroptosis has been implicated as a mechanism of Sertoli cell death. The issue in studying the relationship between hepatitis B virus and Sertoli cell ferroptosis has not yet been addressed.ObjectivesTo explore the mechanisms underlying ferroptosis in hepatitis B virus‐exposed Sertoli cells.Materials and methodsHuman Sertoli cells were treated in vitro with levels of 25, 50, and 100 μg/mL of hepatitis B virus surface protein (HBs). Cell viability and levels of glutathione, malondialdehyde, cellular ferrous ion (Fe2+), lipid peroxidation, and N6‐methyladenosine in Sertoli cells were detected. The level of glutathione peroxidase 4, transferrin receptor 1, ferritin heavy chain, tripartite motif (TRIM) 37, methyltransferase like 3, and insulin‐like growth factor 2 mRNA binding protein 2 was examined. Cell transfection was carried out to alter expression of ferroptosis‐related proteins. qPCR and immunoblotting were performed to measure protein expression level. Immunoprecipitation was applied to determine the protein and protein‐RNA interaction. Luminescence analysis was performed to identify the target of methyltransferase like 3.ResultsHBs exposure triggered ferroptosis featured with increased intracellular Fe2+ ion, reduced cell viability and expression of glutathione peroxidase 4 in Sertoli cells. HBs treatment significantly increased TRIM37 expression, which suppressed glutathione peroxidase 4 expression through ubiquitination. TRIM37 silencing attenuated the effect of HBs exposure‐regulated cell viability and ferroptosis. HBs upregulated N6‐methyladenosine modification in TRIM37 3′‐UTR by increasing methyltransferase like 3 expression. The binding of N6‐methyladenosine reader insulin‐like growth factor 2 mRNA binding protein 2 and TRIM37 3′‐UTR enhanced the stability of TRIM37 mRNA.ConclusionHBs can decrease human Sertoli cell viability by promoting ferroptosis induced by the loss of glutathione peroxidase 4 activity through TRIM37‐mediated ubiquitination of glutathione peroxidase 4. The findings highlight the role of TRIM37/glutathione peroxidase 4 signaling responsible for ferroptosis regulation in hepatitis B virus‐infected Sertoli cells.

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Hepatitis B virus surface protein induces oxidative stress by increasing peroxides and inhibiting antioxidant defences in human spermatozoa

Cited by 8 publications

References 50 publications

Maternal Preconception Hepatitis B Virus Infection and Risk of Congenital Heart Diseases in Offspring Among Chinese Women Aged 20 to 49 Years

Maternal Preconception Hepatitis B Virus Infection and Risk of Congenital Heart Diseases in Offspring Among Chinese Women Aged 20 to 49 Years

Antiviral Potential of Melissa officinalis L.: A Literature Review

The role of hepatitis B virus surface protein in inducing Sertoli cell ferroptosis

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