Hepatitis B virus genotypes circulating in Brazil: molecular characterization of genotype F isolates

Mello, Francisco Campello do Amaral; Souto, Francisco José Dutra; Nabuco, Leticia Cancella; Villela-Nogueira, Cristiane Alves; Coelho, H.S.M.; Franz, Helena Cristina F.; Saraiva, João Carlos Pina; Virgolino, Helaine; Motta-Castro, Ana Rita Coimbra; Melo, Mabel M M; Martins, Regina Maria Bringel; Gomes, Saint Clair

doi:10.1186/1471-2180-7-103

Cited by 109 publications

(164 citation statements)

References 57 publications

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“…Although no comparable data have been published, the proportion of occult HBV infection identifi ed was similar to that reported in a community of African origin (1.7%), which utilized similar methods for the detection of HBV DNA 29 . The HBV genotypes/ subtypes A/A1, D/D3 and F/F2 were identifi ed in the three HBV DNA-positive serum samples, consistent with those circulating in the central-western region and other regions in Brazil 19,30,31 . Furthermore, it is noteworthy that the identifi ed genotype F/subtype F2 may indicate movement of the population from the Amazon region, where the frequency of this genotype/subtype is considerable 30,32 .…”

Section: Discussionmentioning

confidence: 74%

Epidemiology of hepatitis B virus infection among recyclable waste collectors in central Brazil

Marinho

Lopes

Teles

et al. 2014

Rev. Soc. Bras. Med. Trop.

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introduction:The collection of recyclable waste materials is a widespread activity among the urban poor. Today, this occupation attracts an increasingly large number of individuals. Despite its economic and environmental importance, this activity is associated with unsafe and unhealthy working conditions. The aim of this study was to investigate the seroepidemiological profi le of hepatitis B virus (HBV) infection in a population of recyclable waste collectors in central Brazil. Methods: Recyclable waste collectors from all 15 recycling cooperatives in Goiânia City were invited to participate in the study. The participants (n = 431) were interviewed and screened for hepatitis B surface antigen (HBsAg) and antibodies against HBsAg (anti-HBs) and hepatitis B core antigen (anti-HBc) by enzyme-linked immunosorbent assay (ELISA). HBsAg-and anti-HBc-positive samples were tested for HBV DNA and genotyped. Results: The overall prevalence of HBV infection (HBsAg-and/or anti-HBc-positive) was 12.8%. An age over 40 years and illicit drug use were associated with HBV infection. HBV DNA was detected in 2/3 HBsAg-positive samples and in 1/52 anti-HBc-positive/HBsAg-negative samples (an occult HBV infection rate of 1.9%), in which the genotypes/subgenotypes A/A1, D/D3 and F/F2 were identifi ed. Only 12.3% of the recyclable waste collectors had serological evidence of previous HBV vaccination. Conclusions: These fi ndings highlight the vulnerability of recyclable waste collectors to HBV infection and reinforce the importance of public health policies that address the health and safety of this socially vulnerable population.

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Section: Discussionmentioning

confidence: 74%

Epidemiology of hepatitis B virus infection among recyclable waste collectors in central Brazil

Marinho

Lopes

Teles

et al. 2014

Rev. Soc. Bras. Med. Trop.

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“…Studies on the distribution of HBV genotypes in Brazil and the Amazon also show that in the northern region, genotype A is the most common, followed by genotype D and genotype F 12,[17][18][19] . However, other studies performed in the Brazilian Amazon revealed that genotype F is more prevalent [20][21][22] .…”

Section: Ethical Considerationsmentioning

confidence: 99%

Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

Dias

Oliveira

Castilho

et al. 2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Hepatitis B virus (HBV) infection is a serious public health issue worldwide. Hepatitis B virus is classified into eight genotypes, varying from A to H, with distinct geographical distributions. In Brazil, the most frequent genotypes are A, D, and F. METHODS: This study aimed to characterize the HBV genotypes in cases of hepatitis B virus and hepatitis D virus (HDV) co-infections in an endemic area in the Western Brazilian Amazon. We analyzed 86 serum samples reactive for HBsAg from indigenous and non-indigenous populations obtained from previous serological surveys. RESULTS: Of the 86 reactive serum samples, 39 were found to be HBV-DNA-positive by semi-nested PCR. The genotypes were established by sequencing the amplified S gene region. We obtained 20 sequences classified into three genotypes: A, D, and F. Genotype A was the most frequent (60%), followed by D (35%) and F (5%). CONCLUSIONS: The distribution of the HBV genotypes reflected the pattern of historical occupation of the region.

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“…Few genotype data are available in Brazil, but it is known that A, D and F are the main genotypes circulating in the country. MELLO et al (2007) identified genotypes A, D and F among 303 HBV isolates which were obtained from all geographic regions of Brazil, genotype D being the most prevalent in the south region (84.2%). SITNIK et al (2004), studying 103 samples from patients with chronic hepatitis B originating from different regions of Brazil, found genotypes A (49.5%), B (2.9%), C (13.6%), D (24.3%), and F (9.7%).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance

Bertolini

Ribeiro

Lemos

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe present study investigated if hepatitis B virus (HBV) mutants circulate in the southwestern region of the State of Paraná, Brazil, by analyzing samples from children who received immunoprophylaxis but were born to HBV carrier mothers. Samples from 25 children were screened for HBV serum markers and for HBV DNA by PCR. Only one sample was positive for HBsAg, anti-HBs and HBV DNA, although the child had been vaccinated. Analysis of the S gene sequence of this sample showed the presence of a proline at position 105, a serine at position 114, three threonines at positions 115, 116 and 140, and a glutamine at position 129. The presence of these amino acids, except for serine at position 114, has been related to monoclonal or polyclonal therapy with anti-HBs after liver transplantation, whereas the presence of threonine at position 116 has been described in immunized children from Singapore. This finding demonstrates the possible circulation of HBV strains resistant to hepatitis B immunoprophylaxis in southwestern Paraná, Brazil. The genotype of the sample was identified as genotype D, which is frequently found in the region studied. Since 36% of the children had received incomplete or no immunoprophylaxis, more extensive follow-up of children born to HBsAg-positive mothers is needed.

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Hepatitis B virus genotypes circulating in Brazil: molecular characterization of genotype F isolates

Cited by 109 publications

References 57 publications

Epidemiology of hepatitis B virus infection among recyclable waste collectors in central Brazil

Epidemiology of hepatitis B virus infection among recyclable waste collectors in central Brazil

Molecular characterization of the hepatitis B virus in autochthonous and endogenous populations in the Western Brazilian Amazon

Characterization of a Hepatitis B virus strain in southwestern Paraná, Brazil, presenting mutations previously associated with anti-HBs Resistance

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