Hepatitis B virus genotype in Iranian patients with hepatocellular carcinoma

Aghakhani, Arezoo; Hamkar, Rasool; Zamani, Nastaran; Eslamifar, Ali; Banifazl, Mohammad; Saadat, Alireza; Sofian, Masoomeh; Adibi, Ladan; Irani, Nemat; Mehryar, Matin; Akbari, Hassan; Purkhayat, Seyed Mohammad Javad; Ramezani, Amitis

doi:10.1016/j.ijid.2008.10.009

Cited by 16 publications

(15 citation statements)

References 32 publications

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“…Previous findings indicated that there may be an association between the geographical distribution of HDV and HBV genotypes (38). As all reports from Iran showing that genotype D is the only detectable genotype in all clinical forms of HBV infection in this country (41, 42). It is expected that HDV genotype I was detected as predominant genotype in this study.…”

Section: Discussionsupporting

confidence: 57%

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

et al. 2013

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BackgroundHepatitis D virus (HDV) is a defective virus dependent on hepatitis B virus (HBV) for its replication. Due to HDV transmission routes, patients undergoing hemodialysis and those with HIV infection are at risk of acquiring HDV.ObjectivesThis study was aimed to determine the frequency and genotype of HDV infection among patients with HIV infection and those undergoing hemodialysis.Patients and Methods720 cases including 120 patients undergoing hemodialysis, and 600 patients with HIV infection were studied. All cases with positive results for HBsAg were evaluated for the presence of anti-HDV antibodies. Samples with Anti-HDV positive results were subjected to nested PCR for HDV-RNA confirmation, and sequenced for HDV genotype determination.ResultsHBsAg was found in 9 (7.5%) of 120 patients undergoing hemodialysis, and 9 (1.5%) of 600 patients with HIV infection. 3 (33.3%) of patients undergoing hemodialysis with positive results for HBsAg, and 5 (55.5%) of cases with HIV infection and positive results for HBsAg, had positive findings for anti-HDV which were then subjected to nested PCR. The amplification results confirmed that in 3 (37.5%) samples HDV-RNA was detected. Overall 2.5% of patients undergoing hemodialysis, and 0.8% of cases infected with HIV had positive results for anti-HDV and 1.7% and 0.2% of cases undergoing hemodialysis and patients infected with HIV had positive findings for HDV-RNA respectively. All of the HDV isolates were clustered in clade 1.ConclusionsThe survey showed that overall HDV frequency was not high in our high risk cases. Therefore, practitioners and health care managers should become aware of the risk of dual infection with HBV and HDV especially in high risk patients.

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Section: Discussionsupporting

confidence: 57%

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

et al. 2013

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“…The D2 sub-genotype and G, A1, D1 and D5 sub-genotypes have been already reported from India ( 28 , 29 ). The Genotype D has been already reported in Iran ( 30 ); HBV genotype D is predominant among the Iranian neighboring countries including Afghanistan ( 31 ), UAE ( 32 ), and Pakistan ( 33 ). The remaining 108 (90%) patients were negative for occult HBV infection; however the role of occult hepatitis C virus, which requires further investigation, was not evaluated in the present study ( 34 ).…”

Section: Discussionmentioning

confidence: 95%

Occult Hepatitis B Virus Among the Patients With Abnormal Alanine Transaminase

Makvandi

Neisi

Khalafkhany

et al. 2014

Jundishapur J Microbiol

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Background:The occult hepatitis B infection (OBI) is defined as the presence of hepatitis B virus (HBV) DNA in the sera or in the liver biopsy and the absence of hepatitis B surface antigen (HBsAg) by serological test.Objectives:The current study aimed to evaluate the occult HBV infection by polymerase chain reaction (PCR) and determine HBV genotyping among the patients with abnormal alanine transaminase (ALT) in Ahvaz city, Iran.Patients and Methods:The sera of 120 patients, 54 (45%) females and 66 (55%) males, with abnormal ALT 40-152 IU were collected. All the patients were negative for HBsAg for more than one year. The patients` sera were tested by PCR using primers specified for the S region of HBV. Then the positive PCR products were sequenced to determine HBV genotyping and phylogenic tree.Results:Of these 120 subjects, 12 (10%) patients including 6 (5%) males and 6 (5%) females were found positive for HBV DNA by PCR, which indicated the presence of occult HBV infection among these patients. The sequencing results revealed that genotype D was predominant with sub-genotyping D1 among OBI patients.Conclusions:Occult hepatitis B infection is remarkably prevalent in Ahvaz, Iran, and should be considered as a potential risk factor for the transmission of Hepatitis B Virus throughout the community by the carriers.

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“…No HBV S gene sequences were submitted from Saudi Arabia, Libya, Jordan, Lebanon, Bahrain, Qatar, Oman and Kuwait that did not allow the opportunity to determine the prevalence of premature stop codon in these countries. Most of the HBV sequences were reported from Iran (31)(32)(33) and Turkey (34,35). Only a few sequences were reported from Syria, Israel and Iraq, resulting in a wide confidence interval in their estimation of S gene mutation rate.…”

Section: Discussionmentioning

confidence: 99%

HBV S Gene Premature Stop Codon in Strains From Middle Eastern Patients

Vaezjalali

Rezaee

Goudarzi

2012

Arch Clin Infect Dis

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Background: We have previously reported on a premature stop codon in hepatitis B Virus (HBV) S gene among Iranian patients. This mutation may cause undetectable HBV by conventional ELISA methods. Objectives: In this study, we aimed to determine the presence of premature stop codon in HBV S gene from Middle Eastern countries with predominant HBV genotype D, subtype ayw2. Materials and Methods: Submitted HBV sequences to NCBI genome database from Middle Eastern countries (Iran, Iraq, Turkey, Pakistan, Afghanistan, United Arab Emirates (UAE) and Yemen) were retrieved. The S genes of submitted HBV sequences were analyzed by the Bioedit software to evaluate the genotype and premature stop codon in the S gene. Results: Premature stop codon in the S gene was observed for all countries with appropriate sequences for analysis. The frequency of mutant strains to total evaluated sequences was 17/711, 1/2, 4/110 and 1/30 for Iran, Iraq, Turkey and Yemen, respectively. In other countries (Pakistan, Afghanistan and UAE), there were no submitted sequences or the submitted sequences were inappropriate for analysis. Moreover, this mutation in the S gene of HBV was derived from 2 blood donors. Conclusions: Premature stop codon in the S gene was observed in all countries with evaluable HBV genome sequences. Coexistence of detectable hepatitis B surface antigen (HBsAg) and S gene premature stop codon was inconsistent with other studies. Investigations on yield truncated HBsAg are suggested to determine if they can affect ELISA HBsAg results.

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Hepatitis B virus genotype in Iranian patients with hepatocellular carcinoma

Abstract: Our results concur with other reports from Iran, all showing that genotype D is the only detectable genotype in the different clinical forms of HBV infection in this country.

Cited by 16 publications

References 32 publications

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

Frequency and Genotype of Hepatitis D Virus Infection in Patients Infected with HIV and Those Undergoing Hemodialysis

Occult Hepatitis B Virus Among the Patients With Abnormal Alanine Transaminase

HBV S Gene Premature Stop Codon in Strains From Middle Eastern Patients

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