Hepatitis B‐related serological events in hematopoietic stem cell transplant patients and efficacy of lamivudine prophylaxis against reactivation

Abstract

Reactivation of remote hepatitis B infection (RHBI) is an important cause of morbidity in hematopoietic cell transplant (HCT) patients. We analyzed the prevalence of RHBI in 205 patients who underwent HCT in our centre, serological events related to hepatitis B virus (HBV) reactivation and role of lamivudine prophylaxis in HCT patients with RHBI. The prevalence of RHBI was 14% (28/205 patients). Of these 28 patients, 15 received lamivudine prophylaxis (14 anti-HBcIgG positive and 1 only anti-HBs positive) while 13 did not receive lamivudine prophylaxis (12 anti-HBs positive and 1 anti-HBcIgG positive). None in prophylaxis group developed HBV reactivation while 12 of 13 in no-prophylaxis group reactivated (P < 0.001). The rate of HBV reactivation was 10% (21/205 patients), which included 9 patients with no evidence of RHBI pre-transplant. We conclude that lamivudine prophylaxis protects against HBV reactivation in HCT patients with evidence of RHBI. Lamivudine prophylaxis should be used not only in patients with anti-HBcIgG positivity but also in those with isolated anti-HBs positivity pre-transplant given the high rate of HBV reactivation in these patients. HBV serology cannot identify all cases with RHBI and therefore does not preclude HBV reactivation post-transplant. Copyright © 2015 John Wiley & Sons, Ltd.

Comprehensive analysis of differential long non-coding RNA and messenger RNA expression in cholelithiasis using high-throughput sequencing and bioinformatics

“…Bowtie2 and Tophat2 were employed to map reads to the Homo sapiens GRCh38 genome ( Langmead and Salzberg, 2012 ; Kim et al, 2013 ). The mapped reads for each sample underwent assembly using StringTie ( Gupta et al, 2016 ). Subsequently, the transcriptomes from all samples were merged to reconstruct a comprehensive transcriptome, achieved through gffcompare ( https://github.com/gpertea/gffcompare/ ).…”

Integrated analysis of differential lncRNA and mRNA expression in cholelithiasis based on high-throughput sequencing and bioinformatics

“…Then sequence quality was also veri ed using fastp We used Bowtie2 30 and Tophat2 31 to map reads to the genome of Homo sapiens GRCh38. The mapped reads of each sample were assembled using StringTie 32 . Then, all transcriptome from all samples were merged to reconstruct a comprehensive transcriptome using gffcompare (https://github.com/gpertea/gffcompare/).…”

HBV Reactivation in Patients Undergoing Hematopoietic Stem Cell Transplantation: A Narrative Review

“…Universal prophylactic anti-HBV therapy can prevent HBVr in HBsAg-negative, anti-HBc-positive patients undergoing HSCT [91,97,98,99]. However, in allo- and auto-HSCT with resolved HBV infection, a retrospective cohort study with a control group demonstrated that a short-term (median 7 months) of lamivudine prophylaxis was not sufficient to prevent HBV reactivation in a median time of 20 months after HSCT [70].…”