Hepatitis B core antibody in volunteer blood donors: Comparison of radioimmunoassay and indirect immunofluorescence

Katchaki, J. N.; Siem, T. H.; Brouwer, R.

doi:10.1002/jmv.1890030405

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…The ratios of anti-HBc positive in blood donors are reported to be 2-3% in the USA (Irwin et al 1977;Nath et al 1983) and the Netherland (Katchaki et al 1979), and 11% in Italy (Ascione et al 1981). As shown in Fig.…”

Section: Discussionmentioning

confidence: 82%

Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.

Uemura

Fukuyama

Nishida

et al. 1986

Tohoku J. Exp. Med.

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A sensitive and specific assay system for anti-HBc, the passive hemagglutination (PHA) method, has been established. The reactivity of PHA cells prepared by conjugating purified recombinant HBcAg-particles with fixed sheep blood cells (SRBC) was highly specific to monoclonal-and polyclonal anti-HBc IgGs. The sensitivity of PHA method was higher than that of radioimmunoassay (RIA). However, uncertainty for the positivity of anti-HBc still remained in plasma with the PHA titers lower than 25. A relatively high ratio (19%, 37 of 196) of anti-HBc-positive plasma, which had been confirmed to be HBsAg negative, was demonstrated in blood donors whose bloods have been considered suitable for transfusion. The hazards of anti-HBcpositive bloods and the importance of anti-HBc detection in plasma are discussed in this paper.

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Section: Discussionmentioning

confidence: 82%

Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.

Uemura

Fukuyama

Nishida

et al. 1986

Tohoku J. Exp. Med.

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“…The overall prevalence of anti-HBc among volunteer blood donors using ra dioimmunoassay or complement fixation tests has been reported as 2.3% in The Neth erlands [10], 15.3% in Greece [15], 33.3% in Italy [2], and 2.7% in the United States [16], The prevalence of anti-HBc among blood donors in 13 countries in the western hemi sphere was 21%, and 5.3% of all blood do nors had anti-HBc as the sole marker of HBV infection [12], A higher prevalence of anti-HBc has been reported in volunteer blood donors who present with a history of hepatitis or transfusion [17] and among some US military populations [8], The observed prevalence of HBsAg is significantly affected by the donor selection practices and depends upon the proportion of blood collected at a particular type of site, e.g., schools and col leges, plants and factories, on military bases, etc., which in turn reflects the different so cioeconomic status of the donor populations [3]. It is not clear from the data available to us if the lower prevalence of anti-HBc in Boston reported here was due to the differ ences in the type of blood collection sites.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis-Associated Markers in the American Red Cross Blood Donor Population

Nath¹,

Pielech²,

Dodd³

1983

Vox Sanguinis

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A total of 20,643 samples ofblood donated to three American Red Cross Blood Service regions were tested for antibodies to hepatitis B core antigen (anti-HBc) in order to evaluate and understand its significance and implications. All 20,643 samples were nonreactive for hepatitis B surface antigen (HBsAg) when screened at the regions. Overall, 2.2% of all samples were found to have anti-HBc, and a total of 16 had anti-HBc in the absence of antibodies to surface antigen (anti-HBs) activity. 1 of the 16 samples with anti-HBc in the absence of anti-HBs was found to have HBsAg when tested by a sensitive procedural modification of the Ausria II test. Samples with low levels of anti-HBc activity were significantly more likely to be negative for anti-HBs.

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“…In such subjects, antibodies of the IgM class are often absent or present in very low titers (9). Determination of anti-HBc has been extensively employed in epidemiological studies (13) and is demonstrated to be a more sensitive indicator than antibody to hepatitis B surface antigen (anti-HBs) of past HBV infection (5).…”

mentioning

confidence: 99%

New principle for the simultaneous detection of total and immunoglobulin M antibodies applied to the measurement of antibody to hepatitis B core antigen

Angarano¹,

Monno²,

Santantonio³

et al. 1984

J Clin Microbiol

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A new test principle for the simultaneous detection of total approximate titers and immunoglobulin M antibodies has been developed and applied to the detection of antibody to hepatitis B core antigen. The method is based on the combination of a competition radioimmunoassay, for the determination of total antibody titer, with an indirect enzyme-linked immunosorbent assay for the determination of single class antibodies. The interference of the rheumatoid factor was avoided by including heat-aggregated immunoglobulin G in the dilution buffer. The specificity, sensitivity, and clinical application of the test are discussed. The results presented suggest that the simultaneous detection of total and immunoglobulin M antibody to hepatitis B core antigen might be helpful in the differentiation between previous and recent or ongoing hepatitis B infection, as well as in the differential diagnosis of acute hepatitis, in monitoring viral activity in chronic infections, and in helping to differentiate acute from chronic infections. The test principle appears applicable in the accurate diagnosis of other infectious diseases by a single test on only one serum sample.

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Hepatitis B core antibody in volunteer blood donors: Comparison of radioimmunoassay and indirect immunofluorescence

Cited by 8 publications

References 12 publications

Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.

Establishment of passive hemagglutination assay (PHA) system for anti-HBc in plasma.

Hepatitis-Associated Markers in the American Red Cross Blood Donor Population

New principle for the simultaneous detection of total and immunoglobulin M antibodies applied to the measurement of antibody to hepatitis B core antigen

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