Hepatic Uptake and Biliary Excretion of Bromsulphthalein in the Dog

Bräuer, Ralph W.; Pessotti, Rita L.

doi:10.1152/ajplegacy.1950.162.3.565

Cited by 69 publications

(26 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Owen (21) found no BSP in the peripheral blood after gastric or duodenal instillation of BSP. Cohn and associates (4,5) 20 per cent, which is in agreement with the data of Brauer and Pessotti (22). Correcting the data obtained by the BSP method for this 20 per cent loss of the dye from the peripheral blood, the hepatic blood flow would be 24.5 ml./ Kg./min.…”

Section: Discussionsupporting

confidence: 87%

Measurement of Hepatic Blood Flow in the Dog by the Bromsulphalein Method 12

Werner¹,

Horvath²

1952

J. Clin. Invest.

View full text Add to dashboard Cite

Estimation of hepatic blood flow by the bromsulphalein method appears to be based upon meager experimental evidence. Bradley, Ingelfinger, Bradley, and Curry (1) proposed a formula for the estimation of hepatic blood flow in man by measuring the hepatic extraction of bromsulphalein. Myers (2) compared the hepatic blood flow obtained by this method with the hepatic flow determined by urea formation. These An indwelling needle or catheter was also placed in a branch of the femoral artery. All animals were heparinized, 5 mg./Kg. Blood pressures were recorded continuously by Statham strain gauges simultaneously from the femoral artery, right heart, and hepatic vein. A priming dose of bromsulphalein (BSP) was administered to raise the plasma level rapidly and to shorten the time required for equilibration. The priming dose of BSP was followed immediately by infusion of BISP at a constant rate for a period of 80 minutes.In order to maintain a peripheral BSP concentration of less than -mg. per cent or greater than 1 mg.per cent, two rates or two concentrations of BSP infusions were given. The flow was controlled by the use of a Graubard Peterson flowrator. A sample of the infusion fluid was always taken for later analysis. Hepatic venous and peripheral arterial samples were withdrawn simultaneously at 10-minute intervals during the 40 to 80 minutes of each observation.Five ml. of blood were collected in a dry syringe following preliminary flushing of the catheter by withdrawal of several ml. of blood. Ilemolyzed samples were discarded. The hematocrit value was determined by the usual Wintrobe tube technique from samples of blood obtained at the midpoint of measurements of hepatic blood flow. Blood samples were also obtained from the femoral artery and right heart for the estimation of cardiac output by the Fick principle 60 minutes after the infusion had begun. The data on blood pressures and cardiac outputs are being reported in a separate communication. At the end of each period of observation incisions were sutured, 300,000 units of procaine penicillin administered and the dog returned to its cage. No animal was employed either for other observations or repeated hepatic observations until at least two or more weeks had elapsed.BSP was measured calorimetrically in the macro unit of the Evelyn photoelectric colorimeter using filter 580.The calibration curve and the BSP solutions employed in all experiments were prepared from pure BSP powder (Hynson, Westcott, and Dunning).One ml. of plasma was pipetted into an Evelyn colorimeter tube and the volume made up to 10 ml. with isotonic saline. After the initial "blank" reading, 2 drops of concentrated NaOH were added, the mixture well agitated, and a reading taken within one minute of adding the alkali. The BSP concentration was obtained from the galvanometer reading and the calibration curve. All determinations were made in duplicate.433

show abstract

Section: Discussionsupporting

confidence: 87%

Measurement of Hepatic Blood Flow in the Dog by the Bromsulphalein Method 12

Werner¹,

Horvath²

1952

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Assuming a comparatively constant hepatic blood flow, this represents at least a 16-fold range in the quantity of BSP presented to the liver per unit time. In view of the variation in biliary BSP concentration encountered in these dogs, the limiting factor in BSP transport does not appear to be a fixed biliary concentration maximum as suggested by the studies of Brauer and Pessotti for anesthetized animals (3).…”

Section: Toxicitymentioning

confidence: 89%

“…Hepatocellular accumulation or "storage" of BSP was first suggested by the demonstration of a marked delay between removal from the blood and excretion into the bile after the dye was administered intravenously to dogs (2,3), and a similar mechanism in man was inferred from a study of the hepatic removal of successive doses of BSP (4). The accumulation of a related phthalein dye, fluorescein, in hepatic polygonal cells has actually been visualized in living frog and rat livers by the technique of fluorescence microscopy (5,6).…”

mentioning

confidence: 99%

Biliary Transport and Hepatic Storage of Sulfo-Bromophthalein Sodium in the Unanesthetized Dog, in Normal Man, and in Patients With Hepatic Disease *

Wheeler¹,

Meltzer²,

Bradley³

1960

J. Clin. Invest.

273

View full text Add to dashboard Cite

“…Res. 1962, 10, 189;1963, 11, 58) and was "storage compartment," and 2) active secretion into bile (1)(2)(3)(4)(5)(6)(7)(8). The quantity of BSP taken up into the "storage compartment" appears to be directly proportional to plasma concentration (1).…”

Section: Methodsmentioning

confidence: 99%

Alterations in Sulfobromophthalein Sodium-Removal Mechanisms From Blood During Normal Pregnancy*

Combes¹,

Shibata²,

Adams³

et al. 1963

J. Clin. Invest.

View full text Add to dashboard Cite

During the past few years, we have been impressed by the observation that our patients with pre-eclampsia and eclampsia usually demonstrate sulfobromophthalein sodium (BSP) retention in blood in the 45-minute BSP test. On the basis of these findings, we undertook a study of hepatic BSP-removal mechanisms during the course of normal pregnancy. Increased BSP retention in 45 minutes was not detected in normal pregnancy when compared to results obtained in a group of nonpregnant women of comparable age. When hepatic BSP-removal mechanisms were appraised quantitatively by a prolonged-infusion method (1, 2), however, significant changes in both hepatic uptake and excretion of BSP were observed. The following study presents in detail such data obtained from women in various stages of normal pregnancy and in the early postpartum period, and compares these findings with data obtained in a separate group of control nonpregnant women. METHODSThe method used in the present studies was devised by Wheeler and his associates (1,2) and is based on the observations that removal of BSP from plasma depends on the simultaneous operation of at least two separate hepatic mechanisms: 1) uptake of BSP into a hepatic * This work was supported by research grants from the U. S. Public Health Service (H-3439, A-6082), and frcm Parke, Davis & Co. Part of this work has appeared in abstract form (Clin. Res. 1962, 10, 189; 1963, 11, 58) and was presented at the Annual Meeting of the American Association for the Study of Liver Diseases, Chicago, Ill., November, 1962, and "storage compartment," and 2) active secretion into bile (1-8). The quantity of BSP taken up into the "storage compartment" appears to be directly proportional to plasma concentration (1). Thus, uptake into storage during a period of changing plasma concentration can be represented in symbols as S X AP/At, where the "relative storage capacity" S, a proportional factor, is defined as the number of milligrams of BSP stored in the liver per milligram per 100 ml of plasma concentration, and AP/At signifies the rate of change of plasma concentration in milligrams per 100 ml per minute. Secretion of BSP into bile is a rate-limited process characterized by a maximal excretory rate or Tm (1, 2, 8), defined in terms of milligrams per minute. The maximal rate of BSP secretion into bile is achieved when plasma BSP concentration is maintained in excess of 2 to 3 mg per 100 ml during prolonged infusions of BSP (1, 8). At these plasma concentrations, the general equation for the hepatic removal rate of BSP (1), RH, in milligrams per minute, is as follows: RH = S X (AP/At) + Tm.Values for RH and AP/At obtained during two or more different rates of BSP infusion are substituted in the formula, providing a set of simultaneous equations that can be solved for both S and Tm. In practice, values for RH and AP/At were obtained during three separate infusion rates of BSP, each given for an hour. As indicated by Wheeler, when the corresponding values for RH and AP/At are plotted on the ordinate an...

show abstract

Hepatic Uptake and Biliary Excretion of Bromsulphthalein in the Dog

Cited by 69 publications

References 0 publications

Measurement of Hepatic Blood Flow in the Dog by the Bromsulphalein Method 12

Measurement of Hepatic Blood Flow in the Dog by the Bromsulphalein Method 12

Biliary Transport and Hepatic Storage of Sulfo-Bromophthalein Sodium in the Unanesthetized Dog, in Normal Man, and in Patients With Hepatic Disease *

Alterations in Sulfobromophthalein Sodium-Removal Mechanisms From Blood During Normal Pregnancy*

Contact Info

Product

Resources

About