Hepatic PGC-1α has minor regulatory effect on the liver transcriptome and metabolome during high fat high fructose diet and exercise training

Rasmussen, Martin Krøyer; Thøgersen, Rebekka; Lindholm, Pernille Horsbøl; Bertram, Hanne Christine; Pilegaard, Henriette

doi:10.1016/j.gene.2022.147039

Cited by 4 publications

(1 citation statement)

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“…Transcriptomic were conducted using the Affymetrix Clariom S mouse as previously described. [ 54 ] In brief, 100 ng in total of RNA was processed using the GeneChip WT Plus Reagent Kit (Thermo Scientific) according to the manufactures protocol (Thermo Scientific). Obtained cRNA was fragmented, labeled, and loaded into arrays for a 16‐h hybridization.…”

Section: Methodsmentioning

confidence: 99%

Inulin Supplementation Modulates the Hepatic Transcriptome, Metabolome, and Ferritin Content in Ovariectomized Rats

Zhao,

He,

Jakobsen

et al. 2023

Molecular Nutrition Food Res

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ScopeLiver is an important metabolic organ regulating whole‐body homeostasis. This study aims to investigate how prebiotic‐induced changes in the metabolic activity of the gut microbiome (GM) and dietary calcium depletion modulates the hepatic metabolome and transcriptome.Methods and resultsThe serum metabolome, liver metabolome, and transcriptome are determined on samples from ovariectomized (OVX) rats fed a control diet (Control, n = 7), a control diet supplemented with 5% w/w inulin (Inulin, n = 7), or a calcium‐deficient diet (CaDef, n = 7). Inulin fortification is associated with higher serum concentrations of acetate, 3‐hydroxybutyrate, and reduced concentration of dimethyl sulfone, revealing that changes in the metabolic activity of the GM are reflected in circulating metabolites. Metabolomics also reveal that the inulin‐fortified diet results in lower concentrations of hepatic glutamate, serine, and hypoxanthine while transcriptomics reveal accompanying effects on the hepatic expression of ferric iron binding‐related genes. Inulin fortification also induces effects on the hepatic expression of genes involved in olfactory transduction, suggesting that prebiotics regulate liver function through yet unidentified mechanisms involving olfactory receptors.ConclusionInulin ingestion impacts hepatic gene expression and is associated with an upregulation of ferritin synthesis‐related genes and liver ferritin content.

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Section: Methodsmentioning

confidence: 99%