Hepatic Oxidoreduction-Related Genes Are Upregulated by Administration of Hydrogen-Saturated Drinking Water

Nakai, Yuji; Sato, Bunpei; Ushiama, Shota; Okada, Shinji; Abe, Keiko; Arai, Soichi

doi:10.1271/bbb.100819

Cited by 30 publications

(15 citation statements)

References 13 publications

(14 reference statements)

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“…We found that all of these genes were upregulated by HW in the shoots or roots (Figure 4d–j). Hepatic oxidoreduction-related genes in rats have been reported to be upregulated by the administration of hydrogen-saturated drinking water [11]. Our results are consistent with this report and indicate that H 2 may have an antioxidant effect in plants by regulating the expression of genes for antioxidant enzymes, as it does in animal cells.…”

Section: Resultssupporting

confidence: 92%

“…The effects of H 2 on various diseases have been documented for 63 disease models and human diseases in recent years [9]. The mechanism of hydrogen’s effects in various diseases has mainly been attributed to specific scavenging activity for • OH and ONOO − [7], [9], [10], and only a few reports have focused on its gene regulation [11] and signal-modulating activities [9], [12], [13].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Hydrogen Is Involved in Phytohormone Signaling and Stress Responses in Plants

2013

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Molecular hydrogen (H2) metabolism in bacteria and algae has been well studied from an industrial perspective because H2 is viewed as a potential future energy source. A number of clinical trials have recently reported that H2 is a therapeutic antioxidant and signaling molecule. Although H2 metabolism in higher plants was reported in some early studies, its biological effects remain unclear. In this report, the biological effects of H2 and its involvement in plant hormone signaling pathways and stress responses were determined. Antioxidant enzyme activity was found to be increased and the transcription of corresponding genes altered when the effects of H2 on the germination of mung bean seeds treated with phytohormones was investigated. In addition, upregulation of several phytohormone receptor genes and genes that encode a few key factors involved in plant signaling pathways was detected in rice seedlings treated with HW. The transcription of putative rice hydrogenase genes, hydrogenase activity, and endogenous H2 production were also determined. H2 production was found to be induced by abscisic acid, ethylene, and jasmonate acid, salt, and drought stress and was consistent with hydrogenase activity and the expression of putative hydrogenase genes in rice seedlings. Together, these results suggest that H2 may have an effect on rice stress tolerance by modulating the output of hormone signaling pathways.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Molecular Hydrogen Is Involved in Phytohormone Signaling and Stress Responses in Plants

2013

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“…We found that H 2 treatment altered gene expression even in healthy livers. Nakai et al also observed changes of gene expression in healthy rats after oral HRW administration alone and concluded that lipid metabolism-related genes were upregulated [26]. Although we did not observe such changes in gene expression, IPA indicated that NF-jB signaling acted upstream of the down-regulated genes.…”

Section: Discussioncontrasting

confidence: 66%

Simultaneous oral and inhalational intake of molecular hydrogen additively suppresses signaling pathways in rodents

et al. 2015

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Molecular hydrogen (H2) is an agent with potential applications in oxidative stress-related and/or inflammatory disorders. H2 is usually administered by inhaling H2-containing air (HCA) or by oral intake of H2-rich water (HRW). Despite mounting evidence, the molecular mechanism underlying the therapeutic effects and the optimal method of H2 administration remain unclear. Here, we investigated whether H2 affects signaling pathways and gene expression in a dosage- or dose regimen-dependent manner. We first examined the H2 concentrations in blood and organs after its administration and found that oral intake of HRW rapidly but transiently increased H2 concentrations in the liver and atrial blood, while H2 concentrations in arterial blood and the kidney were one-tenth of those in the liver and atrial blood. In contrast, inhalation of HCA increased H2 equally in both atrial and arterial blood. We next examined whether H2 alters gene expression in normal mouse livers using DNA microarray analysis after administration of HCA and HRW. Ingenuity Pathway Analysis revealed that H2 suppressed the expression of nuclear factor-kappa B (NF-κB)-regulated genes. Western blot analysis showed that H2 attenuated ERK, p38 MAPK, and NF-κB signaling in mouse livers. Finally, we evaluated whether the changes in gene expression were influenced by the route of H2 administration and found that the combination of both HRW and HCA had the most potent effects on signaling pathways and gene expression in systemic organs, suggesting that H2 may act not only through a dose-dependent mechanism but also through a complex molecular network.

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“…They showed that H2 suppressed FcɛRI-associated signal transduction and prevented degranulation of mast cell, but not through reducing the ∙ OH, and they also demonstrated that H2 inhibited lipopolysaccharide/interferon γ induced NO production through modulation of signal transduction in the macrophage. An assay of DNA microarrays in the livers detected 548 up-regulated and 695 down-regulated genes in rats after 4 weeks of drinking hydrogen-rich water, and those genes for oxidoreduction-related proteins were enriched in the up-regulated groups 80. However, at present there is no direct evidence could confirm hydrogen gas is a new signalling molecule, which may need further investigations.…”

Section: Future Directionsmentioning

confidence: 87%

Hydrogen as a New Class of Radioprotective Agent

Qian¹,

Shen²,

Chuai³

et al. 2013

Int. J. Biol. Sci.

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It is well known that most of the ionizing radiation-induced damage is caused by hydroxyl radicals (·OH) follows radiolysis of H2O. Molecular hydrogen (H2) has antioxidant activities by selectively reducing ·OH and peroxynitrite(ONOO-). We firstly hypothesized and demonstrated the radioprotective effect of H2 in vitro and in vivo, which was also repeated on different experimental animal models by different departments. A randomized, placebo-controlled study showed that consumption of hydrogen-rich water reduces the biological reaction to radiation-induced oxidative stress without compromising anti-tumor effects. These encouraging results suggested that H2 represents a potentially novel preventative strategy for radiation-induced oxidative injuries. H2 is explosive. Therefore, administration of hydrogen-rich solution (physiological saline/pure water/other solutions saturated with H2) may be more practical in daily life and more suitable for daily consumption. This review focuses on major scientific and clinical advances of hydrogen-rich solution/H2 as a new class of radioprotective agent.

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Hepatic Oxidoreduction-Related Genes Are Upregulated by Administration of Hydrogen-Saturated Drinking Water

Cited by 30 publications

References 13 publications

Molecular Hydrogen Is Involved in Phytohormone Signaling and Stress Responses in Plants

Molecular Hydrogen Is Involved in Phytohormone Signaling and Stress Responses in Plants

Simultaneous oral and inhalational intake of molecular hydrogen additively suppresses signaling pathways in rodents

Hydrogen as a New Class of Radioprotective Agent

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