Hepatic oval ‘stem’ cell in liver regeneration

Oh, Seh Hoon; Hatch, Heather; Petersen, Bryon E.

doi:10.1016/s1084952102001271

Cited by 105 publications

(80 citation statements)

References 38 publications

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“…Oval-cell-mediated regeneration in chronic liver injury is a well-established physiological process [42]. Yet, the origin of these cells remains obscure, a predicament most likely attributable to the diversity of experimental models to investigate liver regeneration.…”

Section: Discussionmentioning

confidence: 99%

Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Corcelle¹,

Stieger

Gjinovci³

et al. 2006

Experimental Cell Research

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Despite extensive studies, the hematopoietic versus hepatic origin of liver progenitor oval cells remains controversial. The aim of this study was to determine the origin of such cells after liver injury and to establish an oval cell line. Rat liver injury was induced by subcutaneous insertion of 2-AAF pellets for 7 days with subsequent injection of CCl 4 . Livers were removed 9 to 13 days post-CCl 4 treatment. Immunohistochemistry was performed using anti-c-kit, OV6, Thy1, CK19, AFP, vWF and Rab3b. Isolated non-parenchymal cells were grown on mouse embryonic fibroblast, and their gene expression profile was characterized by RT-PCR. We identified a subpopulation of OV6/CK19/Rab3b-expressing cells that was activated in the periportal region of traumatized livers. We also characterized a second subpopulation that expressed the HSCs marker c-kit but not Thy1. Although we successfully isolated both cell types, OV6/CK19/Rab3b + cells fail to propagate while c-kit + -HSCs appeared to proliferate for up to 7 weeks. Cells formed clusters which expressed c-kit, Thy1 and albumin. Our results indicate that a bona fide oval progenitor cell population resides within the liver and is distinct from c-kit + -HSCs. Oval cells require the hepatic niche to proliferate, while cells mobilized from the circulation proliferate and transdifferentiate into hepatocytes without evidence of cell fusion.

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Section: Discussionmentioning

confidence: 99%

Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins

Corcelle¹,

Stieger

Gjinovci³

et al. 2006

Experimental Cell Research

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“…109 The promise of liver stem cells lie in their potential to provide a continual and readily available source of liver cells that can be used for gene therapy, cellular transplant, bioartificial liver-assisted devices, drug toxicology testing, and use as an in vitro model to understand the developmental biology of the liver. 5,6 Reproducible stem cell programming, either embryonic or adult, will open a variety of clinical application strategies for the treatment of tissue injuries, degenerated organs, and body components, respectively, in the future. The use of stem cells in humans, present biological advantages and disadvantages related to different types of stem cells and ethical aspects connected to the ES use.…”

Section: Potential Applications Of Stem Cells In Hepatologymentioning

confidence: 99%

“…It has been estimated that complete renewal of liver tissue takes place in about a year. 5,6 Replacement of lost liver tissues is accomplished by proliferation of mature hepatocytes, hepatic oval stem cells differentiation, and sinusoidal cells as support. 5,6 Hepatic oval stem cells are capable of evolving, into hepatocytes or bile duct epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 Replacement of lost liver tissues is accomplished by proliferation of mature hepatocytes, hepatic oval stem cells differentiation, and sinusoidal cells as support. 5,6 Hepatic oval stem cells are capable of evolving, into hepatocytes or bile duct epithelial cells. 6 They display a distinct phenotype and have been shown to be a bipotential progenitor of two types of epithelial cells found in the liver, hepatocytes and bile ductular cells.…”

Section: Introductionmentioning

confidence: 99%

“…5,6 Hepatic oval stem cells are capable of evolving, into hepatocytes or bile duct epithelial cells. 6 They display a distinct phenotype and have been shown to be a bipotential progenitor of two types of epithelial cells found in the liver, hepatocytes and bile ductular cells. [7][8][9] Bone marrow (BM) stem cells have recently been shown not to be a potential source of the hepatic oval cells; rather the source of oval cells and small hepatocytes in the injured liver are endogenous liver progenitors which do not arise through trans-differentiation from BM cells.…”

Section: Introductionmentioning

confidence: 99%

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Pluripotent plasticity of stem cells and liver repopulation

Gennero

Roos

Sperber

et al. 2010

Cell Biochemistry & Function

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Different types of stem cells have a role in liver regeneration or fibrous repair during and after several liver diseases. Otherwise, the origin of hepatic and/or extra-hepatic stem cells in reactive liver repopulation is under controversy. The ability of the human body to self-repair and replace the cells and tissues of some organs is often evident. It has been estimated that complete renewal of liver tissue takes place in about a year. Replacement of lost liver tissues is accomplished by proliferation of mature hepatocytes, hepatic oval stem cells differentiation, and sinusoidal cells as support. Hepatic oval cells display a distinct phenotype and have been shown to be a bipotential progenitor of two types of epithelial cells found in the liver, hepatocytes, and bile ductular cells. In gastroenterology and hepatology, the first attempts to translate stem cell basic research into novel therapeutic strategies have been made for the treatment of several disorders, such as inflammatory bowel diseases, diabetes mellitus, celiachy, and acute or chronic hepatopaties. In the future, pluripotent plasticity of stem cells will open a variety of clinical application strategies for the treatment of tissue injuries, degenerated organs. The promise of liver stem cells lie in their potential to provide a continuous and readily available source of liver cells that can be used for gene therapy, cell transplant, bio-artificial liver-assisted devices, drug toxicology testing, and use as an in vitro model to understand the developmental biology of the liver.

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