Hepatic n-3/n-6 polyunsaturated fatty acid shift improves hepatic steatosis in farnesoid X receptor-null mice

Miyata, Masaaki; Kinoshita, Yuichi; Shinno, Kouhei; Sugiura, Yoshimasa; Harada, Kazuki

doi:10.1007/s12562-016-0984-x

Cited by 4 publications

(8 citation statements)

References 41 publications

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“…The Fxr-null and wild-type mice were housed under a standard 12-hr light-dark cycle (7 a.m.-7 p.m.). Agematched groups of eight-to nine-week-old female mice were used for all experiments because our previous study demonstrated that female Fxr-null mice were more sensitive to lipid metabolism than male Fxr-null mice (Miyata et al, 2016(Miyata et al, , 2011(Miyata et al, , 2017. The mice were fed standard rodent chow (NMF; Oriental Yeast Co. Ltd., Tokyo, Japan).…”

Section: Animal Treatment Sample Collection and Histological Analysismentioning

confidence: 99%

Taurine attenuates hepatic steatosis in a genetic model of fatty liver disease

et al. 2020

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Mice lacking the farnesoid X receptor (FXR) are used as a genetic model for nonalcoholic fatty liver disease because their livers exhibit hepatic steatosis and inflammation. The influence of taurine drinking on disrupted hepatic function was investigated using female Fxr-null mice. Significant decreases in the levels of hepatic damage-associated diagnostic markers, hepatic triglycerides, non-esterified fatty acids, and total bile acids were found in Fxr-null mice that had drunk water containing 0.5% taurine for four weeks. Hepatic but not serum taurine concentrations were significantly increased in these mice. The expression levels of oxidative stress-related genes (Hmox1 and Gsta1) and fatty acid synthetic genes (Acc1 and Scd1) were significantly decreased in these mice. These results suggest that drinking taurine improves hepatic steatosis and dysfunction caused by a lack of FXR.

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Section: Animal Treatment Sample Collection and Histological Analysismentioning

confidence: 99%

Taurine attenuates hepatic steatosis in a genetic model of fatty liver disease

et al. 2020

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“…In our previous study, significant decreases in hepatic and serum lipid levels, but not serum ALT and ALP activities, hepatic bile acid levels and the ratio of liver weight to body weight were observed in Fxr-null mice fed a 4% fish oil diet (no corn oil), compared to those in Fxr-null mice fed a 4% corn oil diet (Miyata et al, 2016). Feeding of n-3 PUFA alone did not improve hepatic function in Fxr-null mice.…”

Section: Discussionmentioning

confidence: 75%

“…The fatty acid composition was analyzed using gas chromatography (GC-2014: Shimadzu Corporation, Kyoto, Japan) with a TC-FFAP capillary column (30 m × 0.25 mm) (GL Sciences Inc., Tokyo, Japan). Methyl esters were identifi ed by comparing the retention times of standard fatty acids (Miyata et al, 2016).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Fish oil feeding reverses hepatomegaly and disrupted hepatic function due to the lack of FXR signaling

et al. 2017

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-Mice lacking farnesoid X receptor (FXR) are used as a model for nonalcoholic fatty liver disease because their livers exhibit hepatomegaly, hepatic steatosis, and hepatic inflammation. The influence of fish oil feeding on hepatomegaly and disrupted hepatic function was investigated using female Fxr-null mice and wild-type mice fed a fish oil diet (2% fish oil and 2% corn oil) or a control diet (4% corn oil) for 4 weeks. Hepatic n-3 polyunsaturated fatty acid (PUFA) levels, including 22:6 n-3 docosahexaenoic acid (DHA) and 20:5 n-3 eicosapentaenoic acid (EPA) were significantly higher in the fish oil group than those in the control group of Fxr-null mice and wild-type mice. Fxr-null mouse livers of the control group showed a whitish brown coloration, whereas Fxr-null mouse livers of the fish oil group showed a dark brown coloration similar to that of wild-type mice. The liver in Fxr-null mice of the fish oil group was smaller than that of the control group. There was a significant decrease in the levels of hepatic damage-associated diagnostic markers, hepatic and serum bile acids, triglycerides, free fatty acids, and total cholesterol levels in Fxr-null mice because of fish oil feeding. It also reversed elevated mRNA levels of oxidative stress-related genes (Hmox1, Gsta1, and Gsta2) and reduced mRNA levels of transcriptional factors (Pparα and Shp) in Fxr-null mice. These results suggest that fish oil feeding reverses hepatomegaly and disrupted hepatic function due to the lack of FXR signaling.

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“…Fxr-null mice were provided by Dr Frank J. Gonzalez (National Institute of Health, Bethesda, MD) (Sinal et al, 2000). Fish oil (DHA-22k) which was prepared from tuna and bonito, was provided by Maruha Nichiro Corp. (Tokyo, Japan) (Miyata et al, 2016). DHA and EPA contents were 26.7% and 6.7%, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The fatty acid composition was analyzed using gas chromatography (GC-2014: Shimadzu Corporation, Kyoto, Japan) with a TC-FFAP capillary column (30 m × 0.25 mm) (GL Sciences Inc., Tokyo, Japan). Methyl esters were identified by comparison with the retention times of standard fatty acids (Miyata et al, 2016).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Effects of fish oil-enriched Sasa-kamaboko diet in decreasing hepatic lipid levels in a mouse fatty liver disease model

Miyata

Kinoshita

Miyahara

et al. 2018

Fundam. Toxicol. Sci.

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The influence of fish oil-enriched Sasa-kamaboko (a Japanese processed seafood) diet on lipid metabolism was investigated using mice lacking farnesoid X receptor (FXR) as a fatty liver disease model. Sasa-kamaboko (SK) was made from Alaska pollock surimi (fish paste), enriched with fish oil (0%, 2.5%, or 5.0%) and then freeze-dried. Fxr-null mice were fed the dried SK, mixed with AIN-93M chow in the ratio 1:1 (50% SK diet) or 1:3 (25% SK diet), for 4 weeks. Hepatic triglyceride levels, and total cholesterol levels were significantly decreased, which were dependent on the amount of added fish oil in 25% and 50% SK diets. Hepatic fatty acid synthase (Fas), acetyl-CoA carboxylase 1 (Acc1), and stearoyl CoA desaturase 1 (Scd1) mRNA levels and Fas protein levels were decreased in groups fed fish oil-enriched 50% SK diets. Brain and serum non-esterified fatty acid levels were significantly decreased in the groups fed fish oil-enriched 50% SK diet, whereas brain and serum, (but not hepatic) phospholipid levels were significantly increased in the groups. Hepatic, serum and brain n-3 polyunsaturated fatty acid, docosahexaenoic acid (DHA; 22:6 n-3), and eicosapentaenoic acid (EPA; 20:5 n-3) levels (except for brain EPA levels) were significantly increased in groups fed fish oil-enriched 50% SK diet, whereas hepatic and serum mono-unsaturated fatty acid, oleic acid (18:1 n-9), and palmitoleic acid (16:1 n-7) levels were significantly decreased in the groups. These results suggest that a diet containing fish oil-enriched SK enhances hepatic lipid-lowering through the alteration of hepatic fatty acid composition in a fatty liver disease mouse model.

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Hepatic n-3/n-6 polyunsaturated fatty acid shift improves hepatic steatosis in farnesoid X receptor-null mice

Cited by 4 publications

References 41 publications

Taurine attenuates hepatic steatosis in a genetic model of fatty liver disease

Taurine attenuates hepatic steatosis in a genetic model of fatty liver disease

Fish oil feeding reverses hepatomegaly and disrupted hepatic function due to the lack of FXR signaling

Effects of fish oil-enriched Sasa-kamaboko diet in decreasing hepatic lipid levels in a mouse fatty liver disease model

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