Hepatic-Modulatory Effects of Chicken Liver Hydrolysate-Based Supplement on Autophagy Regulation against Liver Fibrogenesis

Abstract

Chicken-liver hydrolysates (CLHs) have been characterized as performing several biofunctions by our team. This study aimed to investigate if a CLH-based supplement (GBHP01TM) can ameliorate liver fibrogenesis induced by thioacetamide (TAA) treatment. Our results showed that the TAA treatment caused lower body weight gains and enlarged livers, as well as higher serum ALT, AST, and ALP levels (p < 0.05). This liver inflammatory and fibrotic evidence was ameliorated (p < 0.05) by supplementing with GBHP01TM; this partially resulted from its antioxidant abilities, including decreased TBARS values but increased TEAC levels, reduced GSH contents and catalase/GPx activities in the livers of TAA-treated rats (p < 0.05). Additionally, fewer nodules were observed in the appearance of the livers of TAA-treated rats after supplementing with GBHP01TM. Similarly, supplementing GBHP01TM decreased fibrotic scars and the fibrotic score in the livers of TAA-treated rats (p < 0.05). Moreover, the increased hepatic IL-6, IL-1β, and TNF-α levels after TAA treatment were also alleviated by supplementing with GBHP01TM (p < 0.05). Meanwhile, GBHP01TM could decrease the ratio of LC3B II/LC3B I, but upregulated P62 and Rab7 in the livers of TAA-treated rats (p < 0.05). Taking these results together, the CLH-based supplement (GBHP01TM) can be characterized as a natural agent against liver fibrogenesis.

A functional chicken‐liver hydrolysate‐based supplement ameliorates alcohol liver disease via regulation of antioxidation, anti‐inflammation, and antiapoptosis

“…Our team has successfully developed a functional CLH, which is characterized by the antioxidant effect in d ‐galactose induced mice, 4 the lipid‐lowering effect in high‐fat/cholesterol‐diet fed hamsters (8‐week experiment) 23 and long‐term high‐fat‐diet fed mice (20‐week experiment) 24 . In addition, CLH is the major ingredient in GBHP01™, which has recently been proven as a hepatoprotection against liver fibrogenesis 27 . It has been reported that CLH can reduce the lipase activity and increase the bile‐acid binding activity which could hinder the absorption of lipids in diets and increase fecal bile acid outputs 23 .…”

“…24 In addition, CLH is the major ingredient in GBHP01™, which has recently been proven as a hepatoprotection against liver fibrogenesis. 27 It has been reported that CLH can reduce the lipase activity and increase the bile-acid binding activity which could hinder the absorption of lipids in diets and increase fecal bile acid outputs. 23 Alcohol metabolism in livers could make disturbance of lipid metabolism by increasing the NADH/NAD + ratio, thus upregulating lipogenesis but downregulating fatty-acid β oxidation, producing ROS during alcohol metabolism, and also causing inflammatory responses in livers by promoting proinflammatory cytokine secretion.…”

“…Those benefits are attributed to the free amino‐acid profile and imidazole‐ring dipeptides, that is, anserine (β‐alanyl‐ N ‐histidine), which demonstrate several biological activities, such as antioxidant antioxidative activities, pH buffering, complexing of dangerous carbonyl compounds, anti‐glycation activities on proteins etc 26 . However, recently a hepatoprotection of a CLH‐based supplement, GBHP01™ against liver fibrogenesis induced by thioacetamide has been reported as well 27 . Hence, in the view of the bio‐functionalities of CLHs, this study aimed to evaluate whether CLH‐based daily supplement (GBHP01™) could protect the liver against chronic alcohol consumption via Lieber‐DeCarli EtOH diet‐fed mice.…”

“…26 However, recently a hepatoprotection of a CLH-based supplement, GBHP01™ against liver fibrogenesis induced by thioacetamide has been reported as well. 27 Hence, in the view of the bio-functionalities of CLHs, this study aimed to evaluate whether CLH-based daily supplement (GBHP01™) could protect the liver against chronic alcohol consumption via Lieber-DeCarli EtOH diet-fed mice. The investigations focused on the antioxidative anti-inflammatory, anti-fibrotic, and antiapoptosis effects of this CLH-based daily supplement.…”

An alleviative effect of Lonicerae japonicae flos water extract against liver fibrogenesis in vitro and in vivo

“…The levels of lipid peroxidation thiobarbituric acid reactive substance (TBARS), reduced glutathione (reduced GSH), Trolox equivalent antioxidative capacity (TEAC), as well as activities of superoxide dismutase (SOD), catalase (CAT), and GSH peroxidase (GPx), were assayed based on our previously published methods 17 . The hepatic interleukin‐1β (IL‐1β), IL‐6, and TGF‐β1 contents were determined via an enzyme‐linked immunosorbent assay (ELISA), which complied with the commercial manufacturer's instructions (IL‐1β, IL‐6, and TGF‐β1 kits, R&D system, Minneapolis, MN, USA).…”