Hepatic metallothionein production and resistance to heavy metals by rainbow trout (Salmo gairdneri)—II. Held in a series of contaminated lakes

Roch, M.; McCarter, J. A.

doi:10.1016/0742-8413(84)90133-6

Cited by 36 publications

(23 citation statements)

References 12 publications

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“…Our data for bluegills exposed chronically to known concentrations of copper in the laboratory indicate that the quantities of copper associated with the LMW protein fraction, which contains the MTs, are related to exposure concentration and duration. Our results are similar to those obtained in fish exposed to cadmium (12,15) and to copper (20,22,(30)(31)(32).…”

Section: Discussionsupporting

confidence: 81%

“…Fish have been exposed to mercury, zinc, and copper as well as cadmium . Experiments to determine the effect of copper exposure on MTs in fish have been perforned only on coho salmon (20)(21)(22) and rainbow trout (30)(31)(32). However, copper binding to MTs has been detected in the eel (23), flounder (9), chum salmon (10), killifish (27), skipjack (33), yellowtail (33), blackbream (33), and carp (33).…”

Section: Introductionmentioning

confidence: 99%

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Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Harrison¹,

Lam²

1986

Environmental Health Perspectives

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Livers from bluegills exposed to increased soluble copper (Cu) under field and laboratory conditions were analyzed to determine the concentration and distribution of Cu in metalloproteins of different molecular size. Analyses were performed on bluegills collected from the impoundment of the H. B. Robinson Steam Electric Plant (Florence, SC) near the effluent discharge from the power plant, near the water intake to the cooling system, and from a control pond as well as on bluegills exposed under controlled laboratory conditions. Metalloproteins were separated into low molecular weight (LMW), intermediate molecular weight (IMW), and high molecular weight (HMW) fractions by using high-performance liquid chromatography.In the field-exposed bluegills, Cu concentrations in the LMW, IMW, and HMW fractions were highest in bluegills from the discharge site and lowest in those from the control pond. In the laboratory-exposed bluegills, Cu concentrations in the fractions increased with exposure concentration and time. Concentrations of Cu in the LMW protein fraction and pellet of bluegills exposed to 160 jig Cu/L appeared to plateau with long exposure times, whereas those in the HMW fraction continued to increase. Bluegills maintained in 80 ,ug Cu/L water at pH 5.5 accumulated lower concentrations of Cu in the LMW and pellet fractions and higher amounts in the HMW than in those maintained in 80 jig Cu/L at pH 7.0. Mortality was dependent on exposure concentration and duration and was higher in bluegills maintained in water at pH 5.5 than at pH 7.0.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Harrison¹,

Lam²

1986

Environmental Health Perspectives

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“…15 In a manner similar to bioassays, the application of biomarkers has gone from single biomarker use, to multiple-biomarker use, to use in weight-of-evidence approaches. For example, early environmental studies sought to show exposure of fish to heavy metals by measuring metallothionein, 21 or to show exposure of fish to specific classes of organic contaminants by measuring the CYP4501A enzyme ethoxy-resorufin-ortho-deethylase. 22 Later on, battery applications of biomarkers simultaneously measured in multiple organs/tissues of animals were conducted to maximize ecotoxicity information, which in turn optimized hazard interpretation potential.…”

Section: Biomarkersmentioning

confidence: 99%

“…In this category, for example, we find MT, P4501A and P4503A, responses of which are respectively linked to heavy metal, polycyclic aromatic compounds and drugs and/or naphthenic acids. 21,22,[26][27][28][29] Moreover, biomarkers which can relate to population effects on biota are especially important because of the environmental relevance that they can infer toward specific sources of contamination. Examples include biomarkers such as phagocytosis capacity and vitellogenin induction, both of which have been shown respectively tied to immune-suppressive and reproductive perturbations in bivalves.…”

Section: Biomarkersmentioning

confidence: 99%

“…For example, WOE studies can include toxicity testing on whole organisms, multiple biomarker measurements, and chemistry on water, sediment and tissue, as well as ecological parameters. 25 With continuing development in ecotoxicological research that led to increasing numbers of biomarkers, they have often been grouped into different categories based on the type of ecotoxicity information that they engender (Table 1) 21,[26][27][28][29][30][31][32][33][34][35][36][37][38][39] . Of notable interest are biomarkers that can intimate exposure to a specific class of chemicals.…”

Section: Biomarkersmentioning

confidence: 99%

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Aquatic ecotoxicology: what has been accomplished and what lies ahead? An Eastern Canada historical perspective

Blaise

Gagné

2013

J Xenobiotics

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Our recent history shows that degradation of aquatic ecosystems essentially stems from industrialization, urbanization and increasing human populations. After a first industrial boom in the late 19 th century, contamination pressures on receiving waters now appear to be continual because of expanding economies and technologies developing at the planetary scale. Given the diversity of issues, problems and challenges facing water quality today because of complex waste and chemical discharges into waterways, aquatic ecotoxicology has blossomed with time into a more mature discipline of the environmental sciences. Its two fundamental pillars, bioassays and biomarkers, have become essential tools that allow the determination of numerous and versatile effects measurements. Herein, we demonstrate some of the ways in which these tools have been applied and how they have evolved over the past decades to appraise the ecotoxicity of contaminants impacting aquatic systems. Examples discussed are largely reflective of work conducted in the Environment Canada (EC) laboratories (SaintLawrence Centre, Montréal, Canada). Success stories include improvement of industrial effluent quality contributing to beluga whale population recovery in the Saint-Lawrence River, biomarker field studies conducted with endemic and caged bivalves to more fully comprehend urban effluent adverse effects, and increased discernment on the hazard potential posed by emerging classes of chemicals. Ecotoxicology continues to be confronted with diverse issues and needs related to a myriad of chemical contaminants released to aquatic environments worldwide. To cope with these, ecotoxicology will have to bank on new tools (e.g., toxicogenomics, bio-informatics, modeling) and become more interdisciplinary by taking into account knowledge provided by other disciplines (e.g., ecology, chemistry, climatology, microbiology) in order to more fully understand and adequately interpret hazard. This will be paramount to supply regulators and legislators with the sound and scientifically valid information needed in order to mitigate environmental degradation.

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Acclimation to cadmium toxicity by white suckers: Cadmium binding capacity and metal distribution in gill and liver cytosol

Klaverkamp

Duncan

1987

Enviro Toxic and Chemistry

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Juvenile white suckers (Catostomus commersoni) were exposed for one week to Cd, Zn or Hg at concentrations previously reported to produce acclimation to levels of Cd that would normally induce lethality. Cytosolic fractions from liver and gill filaments were analyzed for their capacity to bind 109Cd and for distribution of Cu, Zn, Cd and Hg. Binding of 109Cd was increased in the cytosolic fraction (peak II), which would contain metallothionein (MTN) from liver of Cd‐exposed and Zn‐exposed fish, and from gill of Hg‐exposed and Zn‐exposed fish. This binding was also increased in the low molecular weight fraction (peak III), which would contain glutathione, from liver cytosol of all metal‐exposed fish and gill cytosol of Cd‐exposed fish. Increases in 109Cd binding in peaks II and III were accompanied by decreases in 109Cd binding in high molecular weight fractions (peak I) containing enzymes from liver cytosol of all metal‐exposed suckers and gill cytosol of Cd‐exposed fish. Exposures to Cd, Zn or Hg produced relative increases in their respective concentrations in peaks I and II, which corresponded to the order of potency (Hg > Cd >> Zn) for inducing acclimation to Cd toxicity. Concentrations of Zn, Cu, Cd and Hg in peak II were used to provide crude estimates of MTN concentrations. Values of 314 and 33 μg MTN/g tissue were obtained for liver and gill, respectively, from non‐exposed fish. Hg, the most potent inducer of acclimation to Cd toxicity, was the only metal that produced increased MTN concentrations in both liver and gill. Zn, the least potent inducer, produced the smallest increase in liver MTN and no increase in gill MTN. Further research that is required to understand the more complex relationships between acclimation to Cd toxicity and cytosolic metal‐binding proteins is discussed.

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Hepatic metallothionein production and resistance to heavy metals by rainbow trout (Salmo gairdneri)—II. Held in a series of contaminated lakes

Cited by 36 publications

References 12 publications

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Aquatic ecotoxicology: what has been accomplished and what lies ahead? An Eastern Canada historical perspective

Acclimation to cadmium toxicity by white suckers: Cadmium binding capacity and metal distribution in gill and liver cytosol

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