Hepatic Metallothionein in Rainbow Trout (<i>Salmo gairdneri</i>) as an Indicator of Metal Pollution in the Campbell River System

Roch, M.; McCarter, J. A.; Matheson, A. T.; Clark, Malcolm; Olafson, Robert W.

doi:10.1139/f82-215

Cited by 122 publications

(36 citation statements)

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“…Our data for bluegills exposed chronically to known concentrations of copper in the laboratory indicate that the quantities of copper associated with the LMW protein fraction, which contains the MTs, are related to exposure concentration and duration. Our results are similar to those obtained in fish exposed to cadmium (12,15) and to copper (20,22,(30)(31)(32).…”

Section: Discussionsupporting

confidence: 89%

“…Fish have been exposed to mercury, zinc, and copper as well as cadmium . Experiments to determine the effect of copper exposure on MTs in fish have been perforned only on coho salmon (20)(21)(22) and rainbow trout (30)(31)(32). However, copper binding to MTs has been detected in the eel (23), flounder (9), chum salmon (10), killifish (27), skipjack (33), yellowtail (33), blackbream (33), and carp (33).…”

Section: Introductionmentioning

confidence: 99%

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Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Harrison¹,

Lam²

1986

Environmental Health Perspectives

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Livers from bluegills exposed to increased soluble copper (Cu) under field and laboratory conditions were analyzed to determine the concentration and distribution of Cu in metalloproteins of different molecular size. Analyses were performed on bluegills collected from the impoundment of the H. B. Robinson Steam Electric Plant (Florence, SC) near the effluent discharge from the power plant, near the water intake to the cooling system, and from a control pond as well as on bluegills exposed under controlled laboratory conditions. Metalloproteins were separated into low molecular weight (LMW), intermediate molecular weight (IMW), and high molecular weight (HMW) fractions by using high-performance liquid chromatography.In the field-exposed bluegills, Cu concentrations in the LMW, IMW, and HMW fractions were highest in bluegills from the discharge site and lowest in those from the control pond. In the laboratory-exposed bluegills, Cu concentrations in the fractions increased with exposure concentration and time. Concentrations of Cu in the LMW protein fraction and pellet of bluegills exposed to 160 jig Cu/L appeared to plateau with long exposure times, whereas those in the HMW fraction continued to increase. Bluegills maintained in 80 ,ug Cu/L water at pH 5.5 accumulated lower concentrations of Cu in the LMW and pellet fractions and higher amounts in the HMW than in those maintained in 80 jig Cu/L at pH 7.0. Mortality was dependent on exposure concentration and duration and was higher in bluegills maintained in water at pH 5.5 than at pH 7.0.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Harrison¹,

Lam²

1986

Environmental Health Perspectives

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“…Several species of freshwater fish respond to metals by synthesizing MT. Roch et al (1982) found a good correlation between hepatic MT levels in rainbow trout caught downstream from a copper mine, and the copper level in the ambient water. Thomas et al (1985) and Kay et al (1986) report that trout, roach and stone loach all showed enhanced levels of MT in response to water-borne zinc and cadmium.…”

Section: Introductionmentioning

confidence: 76%

Metallothionein and metal levels in flounder Platichthys flesus from four f:eid sites and n flounder dosed with water-borne copper

Overnell¹,

Abdullah²

1988

Mar. Ecol. Prog. Ser.

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Concentrations of metallothionein (MT) and of zinc, copper, iron, manganese, cadmium and lead were measured at the GEEP Workshop in liver and kidney of flounder Platichthys flesus from 4 sites in Langesundfjord, Norway, subject to a pollution gradient, and from 4 mesocosm basins dosed with varying levels of copper and dlesel oil. Little difference was found between MT levels in samples from the 3 polluted field sites, though l d n e y MT levels were significantly higher than at the field reference site. Tissue zinc levels were highest in the most heavily polluted fjord site but there was no correlation between hepatic MT and total hepatic zinc. Copper in the mesocosm basins was not accumulated in the flounder livers, nor was liver or ludney MT elevated by the treatment. INTRODUCTIONMetallothionein (MT) is a low molecular weight, heatstable, metal-binding, intracellular protein isolated from various animals. It appears to be ubiquitous in vertebrate tissues, and in mammals is readily inducible by a variety of agents, including copper, cadmium and zinc to which it binds (Kagi & Nordberg 1979, Karin 1985. Several species of freshwater fish respond to metals by synthesizing MT. Roch et al. (1982) found a good correlation between hepatic MT levels in rainbow trout caught downstream from a copper mine, and the copper level in the ambient water. Thomas et al. (1985) and Kay et al. (1986) report that trout, roach and stone loach all showed enhanced levels of MT in response to water-borne zinc and cadmium. However, they found that trout exposed to cadmium alone sequestered the cadmium in the liver as a low molecular weight nonmetallothionein cadmium binding protein. In mammals, various non-metal agents, mostly associated with stress (including noise, injury, bacterial infection and interleukin 1, liver damage and corticosteroid hormones) also induce MT synthesis (Karin 1985). ' Present address: Scottish Marine Biological Associatlon,

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“…Roch, et al (1982) observent que le cadmium est toujours dominant dans les protéines è PME et è FPM . Leur importance est évidemment fonction de la concentration à la source et de la labilité du métal .…”

Section: Caractérisation Des Métalloprotéinesunclassified

Effets de l intoxication au chlorure de cadmium chez la truite mouchetee Salvelinus fontinalis Mitchill

Brillant¹

1993

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Hepatic Metallothionein in Rainbow Trout (Salmo gairdneri) as an Indicator of Metal Pollution in the Campbell River System

Cited by 122 publications

References 0 publications

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Copper-Binding Proteins in Liver of Bluegills Exposed to Increased Soluble Copper under Field and Laboratory Conditions

Metallothionein and metal levels in flounder Platichthys flesus from four f:eid sites and n flounder dosed with water-borne copper

Effets de l intoxication au chlorure de cadmium chez la truite mouchetee Salvelinus fontinalis Mitchill

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