Hepatic Intracellular Osmiophilic Droplets

Ashworth, C. T.; Leonard, J. S.; Eigenbrodt, Edward H.; Wrightsman, F. J.

doi:10.1083/jcb.31.2.301

Cited by 70 publications

(11 citation statements)

References 32 publications

(23 reference statements)

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“…It seems likely that soluble, unbound minerals localized within the matrix would be largely depleted during fixation and dehydration. Membrane-bound minerals, on the other hand, could be at least partially retained even though the formaldehyde-fixed membranes might well be stripped of most of their lipids during acetone dehydration (Ashworth et al, 1966). Rather uniquely, the ultrastructural appearance and macromolecular configuration of mitochondrial membranes appears to depend little, if at all, upon the presence of the lipid components (Fleischer et al, 1967).…”

Section: Al Sh Patterns From Control Mitochondriamentioning

confidence: 97%

“…This agrees with previously reported descriptions of aldehyde-fixed mitochondria (Sabatini et al, 1963;Andre and Marinozzi, 1965). Whether the granules are not present or are simply invisible in this preparation is not known, but the former seems most likely (Ashworth et al, 1966). After 30 min at 5000 or 600°C, sections of the control mitochondria are volatilized completely and leave no ash residue.…”

Section: Lin-fixed and Osmium-fixed Material-the Fate Of Normal Densementioning

confidence: 99%

“…Presumably, membrane lipid was largely retained with osmium (Ashworth et al, 1966). What effect osmium might have on retention of cations bound to phospholipid is, of course, an unanswered question.…”

Section: Al Sh Patterns From Control Mitochondriamentioning

confidence: 99%

“…Without additional heavy metal staining, the osmium-stained granules do not have an electron opacity which even approaches that of small in vitro-produced calcium phosphate granules. Ashworth et al (1966) have shown that the aldehydefixed normal granules cannot be demonstrated even by osmium fixation and staining if this follows dehydration with nonpolar solvents and subsequent rehydration. This behavior is similar to that of membranes and other lipid-containing structures.…”

Section: Normal Dense Granulesmentioning

confidence: 99%

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Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Thomas

Greenawalt

1968

The Journal of Cell Biology

106

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Isolated rat liver mitochondria were incubated in vitro under conditions supporting the massive accumulation of calcium and phosphate. Samples were embedded, thin sectioned, and examined in the electron microscope. The intramitochondrial distribution of insoluble or structure-bound mineral substances was studied by electron microscopy coupled with recently developed techniques of high resolution microincineration. As shown previously, the ion-loaded mitochondria acquire large, internal granules which have inherent electron opacity indicative of high mineral content. Study of ash patterns in preselected areas of sections directly confirmed the high mineral content of the granules, and the appearance of the residues was consistent with the copresence in the granules of some organic material. Other mitochondrial structures were almost devoid of mineral. Thin sections of unincubated control mitochondria also were incinerated. They were found to contain appreciable amounts of intrinsic mineral, seemingly associated with membranes. The normal, dense matrix granules commonly seen in unaltered mitochondria could be seen in intact sections of these control preparations, but after burning no definite correspondence of any ash to the granules could be demonstrated. The normal granules perhaps do not contain mineral. Heating experiments on ash patterns of all the preparations demonstrated the thermal stability and crystallizability of the ash. The crystallized ash of the in vitro-produced dense granules was tentatively shown by electron diffraction to be 3-tricalcium phosphate (whitlockite). This, together with evidence from the literature, suggests that the original, noncrystalline mineral may be a colloidal, subcrystalline precursor of calcium-deficient hydroxyapatite. Experiments were performed on synthetic calcium phosphates for comparison. Other possible applications of the microincineration techniques are briefly discussed.

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Section: Al Sh Patterns From Control Mitochondriamentioning

confidence: 97%

Section: Lin-fixed and Osmium-fixed Material-the Fate Of Normal Densementioning

confidence: 99%

Section: Al Sh Patterns From Control Mitochondriamentioning

confidence: 99%

Section: Normal Dense Granulesmentioning

confidence: 99%

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Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Thomas

Greenawalt

1968

The Journal of Cell Biology

106

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“…Though the nature and function of the mitochondrial body still remains to be elucidated, it is generally accepted that they are lipid-rich (ASHWORTH et al, 1966) and it has also been presumed that they are involved in the transport of cations. ROUILLER (1960) andPEACHEY (1964) suggested that the normal matrix granules prime the accumulation of divalent cations.…”

Section: Discussionmentioning

confidence: 99%

Intramitochondrial Dense Bodies in the Goblet Cells of the Ileum of Muscular Dystrophic Mice

Makita

Matsubara

Kiwaki

1975

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary.Mitochondrial osmiophilic bodies were found in the goblet cells of the small intestine in a group of muscular dystrophic mice. They were not found in any littermate normal control mice.The dense bodies were uniformly round in shape but dense content. Mitochondria with such dense bodies were distributed throughout the goblet cells. Adjacent absorbing epithelial cells had none of such mitochondrial bodies. Though the site of formation of the mitochondrial bodies remains to be investigated, the Golgi complex did not appear to be involved in the transportation of the dense bodies. Digestion of sections with ether-chlorofolm(1:1) slightly decreased the density of the mitochondrial body. Ruthenium red staining was not visible over the dense bodies but trace of Ru was detected by X-ray microanalysis.X-ray microanalysis indicated very few, if any, amounts of P and Ca, and it was not probable that phospholipid and calcium binding protein were the main constitution of these dense bodies.Comparative studies of dystrophic muscle of humans and experimental animals suggested that some alteration in the level of lipid metabolism was involved in muscular dystrophy.In search for morphological evidence of a different level of lipid metabolism in dystrophic mice (MAKITA, KIWAKI and SANDBORN, 1973), the epithelial cells of the small intestine were surveyed in comparatively long lived and severely affected dystrophic mice. In the ileum of four out of seven mice, one or two round dense bodies were found in many mitochondria of goblet cells. Since such dense bodies were not found in the littermate normal control animals they drew our attention, though we could not find similar dense bodies in a parallel study of muscular dystrophic hamster, and subjected the dense bodies to cytochemical and X-ray microanalysis.These dense bodies might be an indication of abnormality in the transportation of lipid-rich substance.Furthermore they might be potential evidence for a relationship between the morphology of mitochondria and the transport of dense substance in goblet cells under specific conditions. Materials and MethodsSeven dystrophic mice of strain C57BL/6J-dydy and four normal controls were sacrificed at 177 days of age. Average body weights of the dystrophic and control mice were 12g and 26g, respectively.Under ether anaesthesia the animals were opened both in the thoracic and abdominal cavities and perfused with Ringer solution through the left cardiac ventricle, followed by injection of fixative consisting of 4%

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Electron microscopic investigation of the source and direction of movement of lipid granules appearing in the hepatic perisinusoidal space following partial hepatectomy,

Parks¹

1969

Am. J. Anat.

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The early sequelae of partial hepatectomy were studied at the ultrastructural level in livers of CFW mice that had been maintained on a normal diet or fed a fat-free diet for four days prior to surgery. Normal-diet animals showed a marked increase in number of small osmiophilic lipid granules in hepatocytes and the space of Disse during the first post-operative hour and at all later intervals studied. This observation generally confirmed similar findings on CSH mice by Trotter ('65), but failed to provide conclusive indications of the source or direction of movement (i.e., from extracellular space into hepatocyte by engulfment, or from hepatocyte to extracellular space by secretion) of the granules in the space of Disse. In livers of fat-free-diet animals, where small lipid granules synthesized in the hepatocyte are recognizable by their osmiophobia (being derived from lipogenesis they are relatively highly saturated), synthesis and secretion by hepatocytes accounted for substantially all lipid granules appearing in the space of Disse during the first three postoperative hours and continued to occur during the fourth hour.This finding demonstrates the importance of the liver cell as a source of lipid granules (presumably very low density lipoproteins) appearing in the space of Disse after hepatectomy. Osmiophilic granules presumed to be of enteric origin, which were present in the space of Disse in very small numbers during the first post-operative hours, increased in number during the fourth hour. The exact fate of these granules and their importance, if any, in the "post-hepatectomy picture" could not be determined from available data.

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Hepatic Intracellular Osmiophilic Droplets

Cited by 70 publications

References 32 publications

Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Intramitochondrial Dense Bodies in the Goblet Cells of the Ileum of Muscular Dystrophic Mice

Electron microscopic investigation of the source and direction of movement of lipid granules appearing in the hepatic perisinusoidal space following partial hepatectomy,

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