Hepatic cystathionase: Immunochemical and electrophoretic studies of the human and rat forms

Pascal, Theresa A.; Tallan, Harris H.; Gillam, Bruce

doi:10.1016/0005-2795(72)90179-1

Cited by 12 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrophoresis proceeded for 2-3 hr at 4O with 0.05 M sodium barbital buffer (pH 8.2) at 10-20 mA/Gelman electrophoresis frame. Antiserum to human liver cystathionase was obtained as described elsewhere (24,25). The antiserum used for these studies was absorbed with lyophilized human plasma proteins (60 mg/ml).…”

Section: Agarose Double Immunodiffusion and Immunoelectrophoresismentioning

confidence: 99%

Cystathionase Deficiency: Evidence for Genetic Heterogeneity in Primary Cystathioninuria

et al. 1978

View full text Add to dashboard Cite

Section: Agarose Double Immunodiffusion and Immunoelectrophoresismentioning

confidence: 99%

Cystathionase Deficiency: Evidence for Genetic Heterogeneity in Primary Cystathioninuria

et al. 1978

View full text Add to dashboard Cite

“…A notable decrease in the latter is observed through the inhibition of the liver enzymes and a reduction in SAM, which is an allosteric inhibitor of methylene-tetrahydrofolate reductase [ 35 ] and an activator of CBS, the first enzyme in the transsulfuration pathway [ 36 ]. Controversially, previous studies using human fetal tissues reported a lack of activity of cystathionine γ-lyase (CGL), which is the final enzyme required for cysteine synthesis in the transsulfuration pathway [ 37 , 38 ]. A lack of functional CGL would limit the sparing effect of Cys in this population.…”

Section: Discussionmentioning

confidence: 99%

Methionine and cysteine oxidation are regulated in a dose dependent manner by dietary Cys intake in neonatal piglets receiving enteral nutrition

Shoveller

Pezzali²,

House³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

Methionine (Met) is an indispensable amino acid (AA) in piglets. Met can synthesize cysteine (Cys), and Cys has the ability to reduce the Met requirement by 40% in piglets. However, whether this sparing effect on Met is facilitated by downregulation of Cys synthesis has not been shown. This study investigated the effects of graded levels of Cys on Met and Cys oxidation, and on plasma AA concentrations. Piglets (n = 32) received a complete elemental diet via gastric catheters prior to being randomly assigned to one of the eight dietary Cys levels (0, 0.05, 0.1, 0.15, 0.2, 0.25, 0.40, 0.50 g kg-1d-1) with an adequate Met concentration (0.25g kg-1d-1). Constant infusion of L-[1-14C]-Met and L-[1-14C]-Cys were performed for 6 h on d 6 and d 8 to determine Met and Cys oxidation, respectively. Met oxidation decreased as Cys intake increased (P<0.05). At higher Cys intakes (0.15 to 0.5g kg-1d-1), Met oxidation decreased (P<0.05) at a slower rate. Cys oxidation was similar (P>0.05) among dietary Cys intakes; however, a significant polynomial relationship was observed between Cys oxidation and intake (P<0.05, R2 = 0.12). Plasma Met concentrations increased (P<0.05) linearly with increasing levels of dietary Cys, while plasma Cys concentrations changed (P<0.05) in a cubic manner and the highest concentrations occurred at the highest intake levels. Increasing dietary levels of Cys resulted in a reduction in Met oxidation until the requirement for the total sulfur AA was met, indicating the sparing capacity by Cys of Met occurs through inhibition of the transsulfuration pathway in neonatal piglets.

show abstract

“…The protection of rat liver cystathionase by PLP has been shown by Garcia-Peregrin and Chatagner (6); rat liver cystathionase is immunologically similar to, but not identical with the human liver enzyme (12). Reiner (13) has postulated on purely theo retical grounds that interaction of an enzyme with its substrate or cofactor could result in decreased stability of the enzyme, but he con sidered this an unlikely reaction.…”

Section: Discussionmentioning

confidence: 99%

Cystathionase Deficiency: the Effect of Cofactor on the Stability of Normal and Abnormal Enzyme from Lymphoid Cell Lines

Ta¹,

Ng²,

Hh³

et al. 1979

Enzyme

Self Cite

View full text Add to dashboard Cite

Heating at 70 °C with and without added pyridoxal phosphate (PLP) had strikingly different effects on cystathionase protein from normal long-term lymphoid cell lines and on the enzyme from cells derived from patients with vitamin-B6-responsive cystathioninuria. PLP added to extracts of normal cells afforded complete protection against heat inactivation, whereas inactivation of the cystathionase protein in extracts obtained from two cystathioninuric lines was greater in the presence of PLP than in its absence.

show abstract

Hepatic cystathionase: Immunochemical and electrophoretic studies of the human and rat forms

Cited by 12 publications

References 16 publications

Cystathionase Deficiency: Evidence for Genetic Heterogeneity in Primary Cystathioninuria

Cystathionase Deficiency: Evidence for Genetic Heterogeneity in Primary Cystathioninuria

Methionine and cysteine oxidation are regulated in a dose dependent manner by dietary Cys intake in neonatal piglets receiving enteral nutrition

Cystathionase Deficiency: the Effect of Cofactor on the Stability of Normal and Abnormal Enzyme from Lymphoid Cell Lines

Contact Info

Product

Resources

About