Hepatic carbonyl sulfide metabolism

Chengelis, Christopher P.; Neal, Robert A.

doi:10.1016/0006-291x(79)91925-9

Cited by 52 publications

(23 citation statements)

References 7 publications

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“…Chengelis and Neal (5) reported that carbonic anhydrase catalyzed the hydrolysis of COS to monothiocarbonate, CO2 and H2S. We confirm the finding that CA catalyzes the hydrolysis of COS (Fig.…”

Section: Resultssupporting

confidence: 80%

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Use of Carbon Oxysulfide, a Structural Analog of CO₂, to Study Active CO₂ Transport in the Cyanobacterium Synechococcus UTEX 625

Miller¹,

Espie²,

Canvin³

1989

Plant Physiol.

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Carbon oxysulfide (carbonyl sulfide, COS) is a close structural analog of CO2. Although hydrolysis of COS (to CO2 and H2S) does occur at alkaline pH (>9), at pH 8.0 the rate of hydrolysis is slow enough to allow investigation of COS as a possible substrate and inhibitor of the active CO2 transport system of Synechococcus UTEX 625. A light-dependent uptake of COS was observed that was inhibited by CO2 and the ATPase inhibitor diethylstilbestrol.The COS taken up by the cells could not be recovered when the lights were tumed off or when acid was added. It was concluded that most of the COS taken up was hydrolyzed by intracellular carbonic anhydrase. The production of H2S was observed and COS removal from the medium was inhibited by ethoxyzolamide. Bovine erythrocyte carbonic anhydrase catalysed the stoichiometric hydrolysis of COS to H2S. The active transport of CO2 was inhibited by COS in an apparently competitive manner. When Natdependent HCO3-transport was allowed in the presence of COS, the extracellular [CO2] rose considerably above the equilibrium level. This CO2 appearing in the medium was derived from the dehydration of transported HCO3-and was leaked from the cells. In the presence of COS the return to the cells of this leaked CO2 was inhibited. These results showed that the Nat-dependent HCO3-transport was not inhibited by COS, whereas active CO2 transport was inhibited. When COS was removed by gassing with N2, a normal pattem of CO2 uptake was observed. The silicone fluid centrifugation method showed that COS (100 micromolar) had little effect upon the initial rate of HC03-transport or CO2 fixation. The steady state rate of CO2 fixation was, however, inhibited about 50% in the presence of COS. This inhibition can be at least partially explained by the significant leakage of CO2 from the cells that occurred when CO2 uptake was inhibited by COS. Neither CS2 nor N20 acted like COS. It is concluded that COS is an effective and selective inhibitor of active CO2 transport.

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Section: Resultssupporting

confidence: 80%

“…There is only one previous report, of which we are aware, that demonstrates catalysis of COS hydrolysis by CA (5). These authors found that bovine erythrocyte CA catalyzed the breakdown of C035S to H235S and '4COS to '4CO2 (5). The process was inhibited by acetazolamide.…”

Section: Discussionmentioning

confidence: 97%

Use of Carbon Oxysulfide, a Structural Analog of CO₂, to Study Active CO₂ Transport in the Cyanobacterium Synechococcus UTEX 625

Miller¹,

Espie²,

Canvin³

1989

Plant Physiol.

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“…This process has been confirmed in rat hepatocytes and erythrocytes (Chengelis & Neal, 1979), higher plants (Protoschill-Krebs & Kesselmeier, 1992), algae (Protoschill-Krebs et al, 1995), lichens (Gries et al, 1994), and cyanobacteria (Miller et al, 1989;Badger & Price, 1990). Micro-organisms possessing the ability to metabolize CO 2 are considered to be involved in COS consumption (Conrad, 1996).…”

Section: Introductionmentioning

confidence: 96%

Degradation of ambient carbonyl sulfide by Mycobacterium spp. in soil

et al. 2008

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“…90,172 Carbonic anhydrase can hydrolyze COS to H 2 S and CO 2 , while monothiocarbonate reacts with carbamyl phosphate synthetase to form thiourea. 31 The zero-valent byproduct S(0) can modify activities of the targeted proteins by forming persulfides with cysteine residues.…”

Section: Interactions With Cytochrome P450mentioning

confidence: 99%

Carbon disulfide. Just toxic or also bioregulatory and/or therapeutic?

et al. 2017

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The overview presented here has the goal of examining whether carbon disulfide (CS 2 ) may play a role as an endogenously generated bioregulator and/or has therapeutic value. The neuro-and reproductive system toxicity of CS 2 has been documented from its long-term use in the viscose rayon industry. CS 2 is also used in the production of dithiocarbamates (DTCs), which are potent fungicides and pesticides, thus raising concern that CS 2 may be an environmental toxin. However, DTCs also have recognized medicinal use in the treatment of heavy metal poisonings as well as having potency for reducing inflammation. Three known small molecule bioregulators (SMBs) nitric oxide, carbon monoxide, and hydrogen sulfide were initially viewed as environmental toxins. Yet each is now recognized as having intricate, though not fully elucidated, biological functions at concentration regimes far lower than the toxic doses. The literature also implies that the mammalian chemical biology of CS 2 has broader implications from inflammatory states to the gut microbiome. On these bases, we suggest that the very nature of CS 2 poisoning may be related to interrupting or overwhelming relevant regulatory or signaling process(es), much like other SMBs. TOC Image 1 CONTENTS

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Hepatic carbonyl sulfide metabolism

Cited by 52 publications

References 7 publications

Use of Carbon Oxysulfide, a Structural Analog of CO₂, to Study Active CO₂ Transport in the Cyanobacterium Synechococcus UTEX 625

Use of Carbon Oxysulfide, a Structural Analog of CO₂, to Study Active CO₂ Transport in the Cyanobacterium Synechococcus UTEX 625

Degradation of ambient carbonyl sulfide by Mycobacterium spp. in soil

Carbon disulfide. Just toxic or also bioregulatory and/or therapeutic?

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Hepatic carbonyl sulfide metabolism

Cited by 52 publications

References 7 publications

Use of Carbon Oxysulfide, a Structural Analog of CO2, to Study Active CO2 Transport in the Cyanobacterium Synechococcus UTEX 625

Use of Carbon Oxysulfide, a Structural Analog of CO2, to Study Active CO2 Transport in the Cyanobacterium Synechococcus UTEX 625

Degradation of ambient carbonyl sulfide by Mycobacterium spp. in soil

Carbon disulfide. Just toxic or also bioregulatory and/or therapeutic?

Contact Info

Product

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Use of Carbon Oxysulfide, a Structural Analog of CO₂, to Study Active CO₂ Transport in the Cyanobacterium Synechococcus UTEX 625

Use of Carbon Oxysulfide, a Structural Analog of CO₂, to Study Active CO₂ Transport in the Cyanobacterium Synechococcus UTEX 625