Hepatic and Adipose Tissue Depot‐Specific Changes in Lipid Metabolism in Late‐Onset Obese (LOB) Rats

Frick, Fredrik; Hume, Randip; Robinson, Iain C. A. F.; Edén, Staffan; Oscarsson, Jan

doi:10.1007/s11745-008-3164-7

Cited by 4 publications

(3 citation statements)

References 50 publications

(80 reference statements)

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“…The liver is a major site of lipogenesis, where most lipogenic genes, including FAS and SCD1, are highly expressed [17]. Moreover, numerous studies have reported that hepatic expression of FAS and SCD1 was increased in obese mice [18]. FAS, which is a central lipogenic gene, provides nonesterified fatty acid substrate for triglyceride synthesis and increases fatty acid uptake with CD36 [19].…”

Section: Resultsmentioning

confidence: 99%

Ethanolic Extract of Taheebo Attenuates Increase in Body Weight and Fatty Liver in Mice Fed a High-Fat Diet

Choi

Ahn

et al. 2014

Molecules

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Abstract:We evaluated whether intake of an ethanolic extract of Taheebo (TBE) from Tabebuia avellanedae protects against body weight increase and fat accumulation in mice with high-fat diet (HFD)-induced obesity. Four-week old male C57BL/6 mice were fed a HFD (25% fat, w/w) for 11 weeks. The diet of control (HFD) mice was supplemented with vehicle (0.5% sodium carboxymethyl cellulose by gavage); the diet of experimental (TBE) mice was supplemented with TBE (150 mg/kg body weight/day by gavage). Mice administered TBE had significantly reduced body weight gain, fat accumulation in the liver, and fat pad weight, compared to HFD mice. Reduced hypertrophy of fat cells was also observed in TBE mice. Mice administered TBE also showed significantly lower serum levels of triglycerides, insulin, and leptin. Lipid profiles and levels of mRNAs and proteins related to lipid metabolism were determined in liver and white adipose tissue of the mice. Expression of mRNA and proteins related to lipogenesis were decreased in TBE-administered mice compared to mice fed HFD alone. These results suggest that TBE inhibits obesity and fat accumulation by regulation of gene expression related to lipid metabolism in HFD-induced obesity in mice.

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Section: Resultsmentioning

confidence: 99%

Ethanolic Extract of Taheebo Attenuates Increase in Body Weight and Fatty Liver in Mice Fed a High-Fat Diet

Choi

Ahn

et al. 2014

Molecules

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“…Interestingly, our laboratory has reported that gastric lavage of soybean trypsin inhibitor, a secretagogue for release of intestinal cholecystokinin (40), will lower ghrelin secretion in fasted rats (22), implying that cholecystokinin might act to inhibit ghrelin secretion. Because physiological changes occur in muscle and liver tissue during consumption of HF diets and obesity (2,16,19), a factor(s) from these tissues might conceivably affect ghrelin homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Ghrelin secretion is not reduced by increased fat mass during diet-induced obesity

Xiang¹,

Reed²,

Wang³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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Ghrelin is a stomach hormone that stimulates growth hormone (GH) secretion, adiposity, and food intake. Gastric ghrelin production and secretion are regulated by caloric intake; ghrelin secretion increases during fasting, decreases with refeeding, and is reduced by diet-induced obesity. The aim of the present study was to test the hypotheses that 1) an increase in body adiposity will play an inhibitory role in the reduction of gastric ghrelin synthesis and secretion during chronic ingestion of a high-fat (HF) diet and 2) chronic ingestion of an HF diet will suppress the rise in circulating ghrelin levels in response to acute fasting. Adult male Sprague-Dawley rats were fed a standard AIN-76A (approximately 5-12% of calories from fat) or an HF (approximately 45% of calories from fat) diet. The effect of increased adiposity on gastric ghrelin homeostasis was assessed by comparison of stomach ghrelin production and plasma ghrelin levels in obese and nonobese rats fed the HF diet. HF diet-fed, nonobese rats were generated by administration of triiodothyronine to lower body fat accumulation. Our findings indicate that an increased fat mass per se does not exert an inhibitory effect on ghrelin homeostasis during ingestion of the HF diet. Additionally, the magnitude of change in plasma ghrelin in response to fasting was not blunted, indicating that a presumed, endogenous signal for activation of ingestive behavior remains intact, despite excess stored calories in HF-fed rats.

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“…DBI responds to lipogenic transcription factors and is involved in lipid metabolism pathways [32]. DGAT2 and SCD are involved in triacylclycerol synthesis [33]. Features of the primer pairs for the14 selected differentiation gene markers and for the ICG are shown in Table S1.…”

Section: Selection Of Osteogenic and Adipogenic Genesmentioning

confidence: 99%

Morphological and Transcriptomic Comparison of Adipose and Bone Marrow Derived Porcine Stem Cells

Monaco¹,

Lima²,

Bionaz³

et al. 2009

TOTERMJ

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Abstract:In the present study we provided a morphological and transcriptomic comparison of adult porcine adiposederived stem cells (ADSC) and bone marrow-derived stem cells (BMSC) as they differentiated in vitro towards the osteogenic and adipogenic lineages for up to 4 weeks. The long term goal of this comparison is to assess the possibility of using ADSC as a potential alternative to BMSC as a source of autologous adult stem cells in human therapies. Our data indicated that ADSC can differentiate into osteocytes and adipocytes similar to BMSC but with some differences. During the osteogenic differentiation both cell types went through morphological changes; however, while ADSC formed predominately osteogenic islands (nodules) in the culture dish, BMSC formed a continuous osteogenic sheet of small nodules. Transcriptomic analysis revealed that both cell types responded to the osteogenic induction. However, BGLAP mRNA expression did not increase in ADSC suggesting, together with the percentage area stained observed for Alizarin Red and von Kossa in ADSC, a lesser mineralization of bone matrix in this cell type compared to BMSC. During the adipogenic induction ADSC as well as BMSC were able to achieve the morphological and transcriptome changes characteristic of the adipogenic lineage. After 7 days of differentiation the expression patterns of DGAT2 and ADFP became greater in ADSC versus BMSC, which agreed with the larger lipid droplets formation observed in the ADSC by Oil Red O staining. Our findings represent an important step towards the assessment of using ADSC as an alternative to BMSC in therapeutic applications.

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Hepatic and Adipose Tissue Depot‐Specific Changes in Lipid Metabolism in Late‐Onset Obese (LOB) Rats

Cited by 4 publications

References 50 publications

Ethanolic Extract of Taheebo Attenuates Increase in Body Weight and Fatty Liver in Mice Fed a High-Fat Diet

Ethanolic Extract of Taheebo Attenuates Increase in Body Weight and Fatty Liver in Mice Fed a High-Fat Diet

Ghrelin secretion is not reduced by increased fat mass during diet-induced obesity

Morphological and Transcriptomic Comparison of Adipose and Bone Marrow Derived Porcine Stem Cells

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