Hepatic 7-ethoxyresorufin O-deethylase Activity in eel (Anguilla Anguilla) from The Thames Estuary and Comparisons with other United Kingdom Estuaries

Doyotte, A; Mitchelmore, Carys L.; Ronisz, D.; McEvoy, J.; Livingstone, David; Peters, L.D.

doi:10.1016/s0025-326x(01)00141-2

Cited by 18 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since our analyses were performed after 7 d of exposure, the different elevation of EROD activity in BaP-and BNFexposed eels might also reflect a different time course of induction for these compounds. The EROD activity of the American eel A. rostrata was induced after 2 d when exposed to BaP and remained constant until day 8 [14]; on the other hand, in primary cultures of rainbow trout hepatocytes treated with BNF, the EROD activity peaked at 48 h and then declined [30], and a similar time course of induction has been recently confirmed in freshwater eels exposed to BNF [15].…”

Section: Discussionmentioning

confidence: 80%

“…Further, deeper insights have been given into pos-sible mechanistic relationships between induction of CYP1A, involvement of specific oxyradicals in perturbing oxidative status, and occurrence of genotoxic damage. The EROD activity of the American eel A. rostrata was induced after 2 d when exposed to BaP and remained constant until day 8 [14]; on the other hand, in primary cultures of rainbow trout hepatocytes treated with BNF, the EROD activity peaked at 48 h and then declined [30], and a similar time course of induction has been recently confirmed in freshwater eels exposed to BNF [15]. Exposure to each of BaP, BNF, Arochlor 1254, and TCDD caused marked induction of EROD (reflective of CYP1A).…”

Section: Discussionmentioning

confidence: 81%

“…Juvenile organisms, still inhabiting marine coastal lagoons, were used, and the exposure concentrations ranged from doses considered ecologically relevant for other Mediterranean fish species [11] to values previously used in similar experiments with eels from freshwater or Atlantic and North American populations [12][13][14][15]. Juvenile organisms, still inhabiting marine coastal lagoons, were used, and the exposure concentrations ranged from doses considered ecologically relevant for other Mediterranean fish species [11] to values previously used in similar experiments with eels from freshwater or Atlantic and North American populations [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…In this work the European eel Anguilla anguilla was exposed to different concentrations of benchmark environmental pollutants, namely, BaP (as polycyclic aromatic hydrocarbon), BNF (a synthetic inducer of cytochrome P450), Arochlor 1254 (a commercial mixture of polychlorinated biphenyls), and 2,3,7,8-tetrachlorodibenzo p-dioxin (the most toxic congener of these organohalogenated compounds). Juvenile organisms, still inhabiting marine coastal lagoons, were used, and the exposure concentrations ranged from doses considered ecologically relevant for other Mediterranean fish species [11] to values previously used in similar experiments with eels from freshwater or Atlantic and North American populations [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla

Regoli

Winston

Gorbi

et al. 2003

Enviro Toxic and Chemistry

162

View full text Add to dashboard Cite

In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[a]pyrene ([BaP], at 0, 0.1, 1, 10, and 50 mg/kg), beta-naphthoflavone ([BNF], at 0, 0.1, 1, 10, and 50 mg/kg), Arochlor 1254 (at 0, 0.1, 1, 10, and 50 mg/kg), and 2,3,7,8-tetrachlorodibenzo p-dioxin ([TCDD], at 0, 0.01, 0.1, 1, and 2 microg/kg). The integral relationships were analyzed between induction of ethoxyresorufin O-deethylase (EROD) activity, its involvement in perturbing oxyradical metabolism, and the role of cytochrome P450 and/or oxidative stress in mediating genotoxic effects. To reveal whether the oxidative status in exposed organisms was altered as a result of chemical exposure, measurements of the main endogenous antioxidant defenses were integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals (*OH). This approach permits discriminating the resistance of a tissue toward different forms of oxyradicals, thereby indicating a differential role for specific reactive oxygen species (ROS) in perturbing the balance between prooxidant and antioxidant mechanisms. All the analyzed chemicals promoted EROD induction (reflective of CYP1A) and altered either the levels or the activities of the antioxidants studied, which might be anticipated to exert alterations in oxyradical metabolism. Analysis of TOSC suggested the prevalence of metabolic oxidative pathways leading to the more reactive *OH on exposure to the chemicals studied. Of these chemicals, enhanced EROD activity correlated with genotoxic damage only in the cases of the nonhalogenated hydrocarbons BaP and BNF. The highest degree of genotoxic damage was consistently observed in organisms in which the capacity to absorb or scavenge OH was lowest. These data suggest a general relationship between oxidative stress and loss of DNA integrity in juvenile eels exposed to the chemicals studied herein.

show abstract

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla

Regoli

Winston

Gorbi

et al. 2003

Enviro Toxic and Chemistry

162

View full text Add to dashboard Cite

show abstract

“…Using the above selection criteria, 94 papers were retained for the analysis of EROD control/reference data [12–105], and 27 of these papers were selected for further analysis of EROD dose‐response relationships [12–38]. The papers chosen for analysis of the EROD control/reference data set had a total of 78 unique author groups (which we equated to individual laboratories), 42 different genera of fish, and 21 different families of fish.…”

Section: Resultsmentioning

confidence: 99%

Statistical analysis of cytochrome P4501A biomarker measurements in fish

Oris

Roberts

2007

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Abstract-Induction of the cytochrome P4501A (CYP1A) enzyme system in fish is a common biomarker of exposure to aromatic hydrocarbons. Induction of CYP1A can be measured at a number of steps in the transcription-translation-functional protein pathway using a variety of techniques. The present study examined the range of these measurements from 94 published papers in an attempt to examine the statistical characteristics of each method. Cytochrome P4501A induction, as measured by catalytic ethoxyresorufin-O-deethylase (EROD) activity, protein levels (enzyme-linked immunosorbent assay, Western blot analysis, and immunohistochemistry), and mRNA levels (Northern blot analysis and reverse transcription-polymerase chain reaction), was analyzed. When possible, the variance structure, effect size determination, and dose-response modeling of each method of measurement in the laboratory and field were examined. Conclusions from this analysis include: 1) Because of interlaboratory and interspecies variability, general end-point determinations will need to be defined in terms of the statistically detectable fold-change of measurements relative to control or reference values, and 2) fold-change in EROD activity provides the most robust measure of the dose responsiveness of aromatic hydrocarbons within specific chemical classes (e.g., polycyclic aromatic hydrocarbons). The relationship between the ability to measure statistical differences in induction level and the biological significance of those measurements has yet to be defined. To utilize these biomarkers in a risk assessment context, this relationship must be addressed at the scientific and management levels.

show abstract

Biomonitoring Studies Performed with European Eel Populations from the Estuaries of Minho, Lima and Douro Rivers (NW Portugal)

Gravato

Faria

Alves

et al. 2008

Advanced Environmental Monitoring

View full text Add to dashboard Cite

Hepatic 7-ethoxyresorufin O-deethylase Activity in eel (Anguilla Anguilla) from The Thames Estuary and Comparisons with other United Kingdom Estuaries

Cited by 18 publications

References 46 publications

Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla

Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel Anguilla anguilla

Statistical analysis of cytochrome P4501A biomarker measurements in fish

Biomonitoring Studies Performed with European Eel Populations from the Estuaries of Minho, Lima and Douro Rivers (NW Portugal)

Contact Info

Product

Resources

About