Heparinized Micropatterned Surfaces for the Spatial Control of Human Mesenchymal Stem Cells

Choi, Jong Hoon; Kim, Tae Eun; Парк, Ки Донг; Kim, Ji Youn; Park, Yongdoo; Sun, Kyung

doi:10.1177/0883911509349143

Cited by 9 publications

(1 citation statement)

References 31 publications

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“…The higher hMSC adhesion and proliferation on pKL surfaces could be related to heparin adsorption since it was previously demonstrated that heparin immobilized on anti-adhesive surfaces increased hMSC adhesion [31].…”

Section: Discussionmentioning

confidence: 92%

Enhancement of hMSC In Vitro Proliferation by Surface Immobilization of a Heparin-Binding Peptide

et al. 2023

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The use of human Mesenchymal Stem Cells (hMSC) as therapeutic agents for advanced clinical therapies relies on their in vitro expansion. Over the last years, several efforts have been made to optimize hMSC culture protocols, namely by mimicking the cell physiological microenvironment, which strongly relies on signals provided by the extracellular matrix (ECM). ECM glycosaminoglycans, such as heparan-sulfate, sequester adhesive proteins and soluble growth factors at the cell membrane, orchestrating signaling pathways that control cell proliferation. Surfaces exposing the synthetic polypeptide poly(L-lysine, L-leucine) (pKL) have previously been shown to bind heparin from human plasma in a selective and concentration-dependent manner. To evaluate its effect on hMSC expansion, pKL was immobilized onto self-assembled monolayers (SAMs). The pKL-SAMs were able to bind heparin, fibronectin and other serum proteins, as demonstrated by quartz crystal microbalance with dissipation (QCM-D) studies. hMSC adhesion and proliferation were significantly increased in pKL-SAMs compared to controls, most probably related to increased heparin and fibronectin binding to pKL surfaces. This proof-of-concept study highlights the potential of pKL surfaces to improve hMSC in vitro expansion possible through selective heparin/serum protein binding at the cell-material interface.

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Section: Discussionmentioning

confidence: 92%

Enhancement of hMSC In Vitro Proliferation by Surface Immobilization of a Heparin-Binding Peptide

et al. 2023

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Aging of porous silicon in physiological conditions: Cell adhesion modes on scaled 1D micropatterns

Noval¹,

Vaquero²,

Quijorna³

et al. 2012

J Biomedical Materials Res

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The surface properties of porous silicon (PSi) evolve rapidly in phosphate-buffered saline. X-ray photoelectron spectra indicate the formation of a Si-OH and C-O enriched surface, which becomes increasingly hydrophilic with aging time. Multiscale stripe micropatterns of Si and PSi have been fabricated by means of a high-energy ion-beam irradiation process. These micropatterns have been aged in physiological conditions and used to analyze human mesenchymal stem cell (hMSC) adhesion. The actin cytoskeleton of hMSCs orients following the uniaxial micropatterns. In the wider Si stripes, hMSCs are dominantly located on Si areas. However, for reduced Si widths, adhesion is avoided on PSi by a split assembly of the actin cytoskeleton on two parallel Si areas. These results confirm that nanostructured Si-OH/C-O-rich surfaces with hydrophilic character are specially adapted for the creation of cell adhesion surface contrasts.

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Surface modification of biomaterials by photochemical immobilization and photograft polymerization to improve hemocompatibility

Feng

Zhao

Zhang

et al. 2010

Front. Chem. Eng. China

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Thrombus formation and blood coagulation are serious problems associated with blood contacting products, such as catheters, vascular grafts, artificial hearts, and heart valves. Recent progresses and strategies to improve the hemocompatibility of biomaterials by surface modification using photochemical immobilization and photograft polymerization are reviewed in this paper. Three approaches to modify biomaterial surfaces for improving the hemocompatibility, i.e., bioinert surfaces, immobilization of anticoagulative substances and biomimetic surfaces, are introduced. The biomimetic amphiphilic phosphorylcholine and Arg-Gly-Asp (RGD) sequence are the most effective and most often employed biomolecules and peptide sequence for improving hemocompatibility of material surfaces. The RGD sequence can enhance adhesion and growth of endothelial cells (ECs) on material surfaces and increase the retention of ECs under flow shear stress conditions. This surface modification is a promising strategy for biomaterials especially for cardiovascular grafts and functional tissue engineered blood vessels.

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Heparinized Micropatterned Surfaces for the Spatial Control of Human Mesenchymal Stem Cells

Cited by 9 publications

References 31 publications

Enhancement of hMSC In Vitro Proliferation by Surface Immobilization of a Heparin-Binding Peptide

Enhancement of hMSC In Vitro Proliferation by Surface Immobilization of a Heparin-Binding Peptide

Aging of porous silicon in physiological conditions: Cell adhesion modes on scaled 1D micropatterns

Surface modification of biomaterials by photochemical immobilization and photograft polymerization to improve hemocompatibility

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