Heparinase‐modified thromboelastography in cats

Cuq, Benoît; Dunn, Marilyn; Bédard, Christian

doi:10.1111/vec.12559

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Our clinical pathology laboratory adhered to current practice recommendations, and its reference range intervals are similar to those of other cat studies. [19][20][21]…”

Section: Data Collectionmentioning

confidence: 99%

Prothrombin and activated partial thromboplastin times, thromboelastography, hematocrit, and platelet count in a feline hemorrhage/over‐resuscitation model using lactated Ringer's solution or 6% tetrastarch 130/0.4

Zeiler,

Dzikiti,

Rioja

et al. 2024

J Vet Emergen Crit Care

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ObjectiveTo describe and compare prothrombin time (PT), activated partial thromboplastin time (aPTT), thromboelastography (TEG), HCT, and platelet count measurements in a hemorrhage/over‐resuscitation model.DesignRandomized crossover study.SettingUniversity teaching hospital.AnimalsSix cats.InterventionsAnesthetized cats underwent 3 treatments at 2‐month intervals. The treatments were as follows: NHR—no controlled hemorrhage and sham resuscitation; LRS—controlled hemorrhage and lactated Ringer's solution (LRS) for resuscitation; and Voluven—controlled hemorrhage and 6% tetrastarch 130/0.4 for resuscitation. The LRS and Voluven were administered at 60 and 20 mL/kg/h, respectively, for 120 minutes. Blood samples were drawn for PT, aPTT, TEG, HCT, and platelet count measurements at a healthy check (T − 7d), after controlled hemorrhage (T0), at 60 and 120 minutes of resuscitation (T60 and T120), and at 24 hours after completion of resuscitation (T24h). Data were analyzed using a general linear mixed model approach (significance was P < 0.05).Measurements and Main ResultsTotal median blood loss (controlled hemorrhage and blood sampling from T0 to T120) at T120 was 11.4, 31.0, and 30.8 mL/kg for NHR, LRS, and Voluven, respectively. PT and aPTT during LRS and Voluven were prolonged at T60 and T120 compared to NHR (P < 0.001). On TEG, the reaction time, kinetic time, and alpha‐angle were within reference intervals for cats at all time points in all treatments, while maximum amplitude was less than the reference interval (40 mm) at T0, T60, and T120 during Voluven and at T60 and T120 during LRS compared to NHR (both P < 0.001). The HCT and platelet count were significantly lower at T60 and T120 during LRS and Voluven compared to NHR (P < 0.001).ConclusionsHypocoagulopathy was observed during hemorrhage and liberal fluid resuscitation. Prolongation of PT and aPPT and decreased clot strength may have been caused by hemodilution and platelet loss.

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“…Our clinical pathology laboratory adhered to current practice recommendations, and its reference range intervals are similar to those of other cat studies. [19][20][21]…”

Section: Data Collectionmentioning

confidence: 99%

Prothrombin and activated partial thromboplastin times, thromboelastography, hematocrit, and platelet count in a feline hemorrhage/over‐resuscitation model using lactated Ringer's solution or 6% tetrastarch 130/0.4

Zeiler,

Dzikiti,

Rioja

et al. 2024

J Vet Emergen Crit Care

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show abstract

“…Recent studies have established TEG reference interval values using activators in healthy cats; however, they have used a combination of 21 G or 22 G needles for blood sampling. 1,4,12–15 It is known that the venepuncture technique also affects the results of coagulation tests in veterinary medicine. 5,6 One author classified the venepuncture difficulty score into four categories in dogs and then reported whether there was a significant difference in TEG parameters between the samples assigned with different difficulty scores.…”

Section: Introductionmentioning

confidence: 99%

Influence of needle gauge and venepuncture difficulty on thromboelastography in healthy cats

Shin

Nam

Song

et al. 2018

Journal of Feline Medicine and Surgery

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Objectives The aim of this study was to evaluate the effect of two differently sized butterfly catheter needles and the effect of venepuncture difficulty on thromboelastography (TEG) results in healthy cats. Methods Twenty-four healthy cats were included. Blood samples were collected from the jugular vein by syringe aspiration via direct venepuncture with 21 G and 22 G butterfly needles. The venepuncture difficulty score was classified into four categories. The first 1.5 ml blood drawn from each subject was discarded before collecting a sample for TEG analysis. TEG analyses were performed on citrated whole blood samples from 17 clinically healthy cats, using assays with kaolin as activators. Among the TEG parameters, reaction time (R), clot formation time (κ), alpha angle (α), maximum amplitude (MA) and global clot strength (G) were recorded from each tracing. Results Seven cats were excluded from the study; results were obtained for the remaining 17 cats. There were no statistically significant differences between the use of two different needles for R ( P = 0.72), κ ( P = 0.74), α ( P = 0.99), MA ( P = 0.08) and G ( P = 0.09). Samples with difficulty scores ⩾1 were not significantly different from samples with difficulty scores of 0 for R ( P = 0.24), κ ( P = 0.65), α ( P = 0.65), MA ( P = 0.72) and G ( P = 0.77). Conclusions and relevance The results of TEG in clinically healthy cats do not differ significantly when using two different gauge needles. There was no significant difference in the TEG results according to venepuncture difficulty scoring.

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The heparinase-linked differential time method allows detection of heparin potency in whole blood with high sensitivity and dynamic range

Zheng

Zhang

et al. 2022

Biosensors and Bioelectronics

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Heparinase‐modified thromboelastography in cats

Abstract: Heparinase modified, TF-activated, TEG reverses heparin effects in feline-citrated blood.

Cited by 3 publications

References 14 publications

Prothrombin and activated partial thromboplastin times, thromboelastography, hematocrit, and platelet count in a feline hemorrhage/over‐resuscitation model using lactated Ringer's solution or 6% tetrastarch 130/0.4

Prothrombin and activated partial thromboplastin times, thromboelastography, hematocrit, and platelet count in a feline hemorrhage/over‐resuscitation model using lactated Ringer's solution or 6% tetrastarch 130/0.4

Influence of needle gauge and venepuncture difficulty on thromboelastography in healthy cats

The heparinase-linked differential time method allows detection of heparin potency in whole blood with high sensitivity and dynamic range

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