Heparin surface treatment of poly(methylmethacrylate) alters adhesion of a Staphylococcus aureus strain: utility of bacterial fatty acid analysis

Arciola, Carla Renata; Radin, L.; Alvergna, Paola; Cenni, E.; Pizzoferrato, Alberto

doi:10.1016/0142-9612(93)90161-t

Cited by 55 publications

(24 citation statements)

References 9 publications

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“…For example, surfaces chemically modified with poly(ethylene glycol) and certain other synthetic polymers can repel (although not kill) microorganisms (1)(2)(3)(4)(5)(6). Alternatively, materials can be impregnated with antimicrobial agents, such as antibiotics, quaternary ammonium compounds, silver ions, or iodine, that are released gradually into the surrounding solution over time and kill microorganisms therein (6)(7)(8)(9).…”

mentioning

confidence: 99%

Designing surfaces that kill bacteria on contact

Tiller

Liao

Lewis

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

1,081

1,011

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Poly(4-vinyl-N-alkylpyridinium bromide) was covalently attached to glass slides to create a surface that kills airborne bacteria on contact. The antibacterial properties were assessed by spraying aqueous suspensions of bacterial cells on the surface, followed by air drying and counting the number of cells remaining viable (i.e., capable of growing colonies). Amino glass slides were acylated with acryloyl chloride, copolymerized with 4-vinylpyridine, and N-alkylated with different alkyl bromides (from propyl to hexadecyl). The resultant surfaces, depending on the alkyl group, were able to kill up to 94 ؎ 4% of Staphylococcus aureus cells sprayed on them. A surface alternatively created by attaching poly(4-vinylpyridine) to a glass slide and alkylating it with hexyl bromide killed 94 ؎ 3% of the deposited S. aureus cells. On surfaces modified with N-hexylated poly(4-vinylpyridine), the numbers of viable cells of another Gram-positive bacterium, Staphylococcus epidermidis, as well as of the Gram-negative bacteria Pseudomonas aeruginosa and Escherichia coli, dropped more than 100-fold compared with the original amino glass. In contrast, the number of viable bacterial cells did not decline significantly after spraying on such common materials as ceramics, plastics, metals, and wood. Because of the ever-growing demand for healthy living, there is a keen interest in materials capable of killing harmful microorganisms. Such materials could be used to coat the surfaces of common objects touched by people in everyday life (e.g., door knobs, children's toys, computer keyboards, telephones, etc.) to render them antiseptic and thus unable to transmit bacterial infections.Because ordinary materials are not antimicrobial, they require modification. For example, surfaces chemically modified with poly(ethylene glycol) and certain other synthetic polymers can repel (although not kill) microorganisms (1-6). Alternatively, materials can be impregnated with antimicrobial agents, such as antibiotics, quaternary ammonium compounds, silver ions, or iodine, that are released gradually into the surrounding solution over time and kill microorganisms therein (6-9). Although these strategies have been verified in aqueous solutions containing bacteria, they would not be expected to be effective against airborne bacteria in the absence of a liquid medium; this situation is especially true for release-based materials (6), which are also liable to become impotent when the leaching antibacterial agent is exhausted.It has been reported (10-13) that various polycations possess antibacterial properties in solution, presumably by interacting with and disrupting bacterial cell membranes. However, this antibacterial activity vanishes when these polycations are crosslinked or otherwise insolubilized (12,14,15). We have hypothesized that their antibacterial properties can be preserved, even after insolubilization, and expressed in a dry state, if the immobilized polycationic chains are sufficiently long and flexible to be able to penetrate the bacterial...

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mentioning

confidence: 99%

Designing surfaces that kill bacteria on contact

Tiller

Liao

Lewis

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

1,081

1,011

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“…The immobilization of hydrophilic polymer monolayers on solids has proven to be an effective method of preventing nonspecific protein adsorption and cell adhesion [14][15][16][17]. Among various molecules, PEG is widely used for providing a biocompatible surface due to the hydrophilic and neutral chemical structure of the polymer.…”

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confidence: 99%

Transport and functional behaviour of poly(ethylene glycol)-modified nanoporous alumina membranes

Lee

Shang

Haasch³

et al. 2005

Nanotechnology

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The development of hybrid organic–inorganic membranes with a low propensity for protein adsorption and highly uniform nanometre size pores is described. Poly(ethylene glycol) (PEG) monolayers were grafted to nanoporous alumina membranes using covalent silane and physical adsorption poly(ethyleneimine) (PEI) immobilization chemistries. X-ray photoelectron spectroscopy (XPS) and electron microscopy were used to investigate the chemical and physical surface properties of the membranes. The adsorption behaviour of a serum albumin on the membranes was characterized with fluorescence spectroscopy and it was determined that the PEG coating reduced nonspecific protein adsorption to a level too small to be measured. The gas and liquid permeabilities of membranes were measured to determine if the surface chemistries changed the functional behaviour of the membranes. Surprisingly, the silane chemistry produced little change in the permeabilities while polymer adsorption resulted in a total loss of water permeability. The diffusion of ovalbumin through the membranes was also measured and compared with a theoretical value. Diffusion of ovalbumin through the silane-PEG-modified membranes was found to be 50% slower than the unmodified membranes, which suggests that the pores are coated with a dense film of PEG. These results suggest that hybrid organic–inorganic membranes can provide significantly improved functional behaviour over existing organic or inorganic membranes.

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“…La heparina ha disminuído la adhesión de bacterias a la superficie de lentes intraoculares. Se supone que la interacción entre S. epidermidis y fibronectina ocurre en un único sitio en sentido C-terminal, el cual es específicamente inhibido por la heparina 70,71 . Estos estudios sugieren un rol de la heparina como un inhibidor específico de la adhesión de S. epidermidis a la superficie de los biomateriales 5 .…”

Section: Infectología Al Díaunclassified

Bases moleculares de la infección asociada a implantes ortopédicos

Contreras

Sepúlveda

2014

Rev. chil. infectol.

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Heparin surface treatment of poly(methylmethacrylate) alters adhesion of a Staphylococcus aureus strain: utility of bacterial fatty acid analysis

Cited by 55 publications

References 9 publications

Designing surfaces that kill bacteria on contact

Designing surfaces that kill bacteria on contact

Transport and functional behaviour of poly(ethylene glycol)-modified nanoporous alumina membranes

Bases moleculares de la infección asociada a implantes ortopédicos

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