Heparin improves organ microcirculatory disturbances in caerulein-induced acute pancreatitis in rats

Dobosz, Marek; Mionskowska, L; Hać, Stanisław; Dobrowolski, Sebastian; Dymecki, Dariusz; Wajda, Z

doi:10.3748/wjg.v10.i17.2553

Cited by 17 publications

(15 citation statements)

References 30 publications

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“…Another important features in the course of pancreatitis is the drop in microperfusion in the pancreatic gland, which in turn could enhance the infl ammatory state. Dobosz et al [29] have demonstrated that heparin improved pancreas microcirculation in rats with cerulean-induced pancreatitis. In this failure, participate vasoactive mediators such as endothelin, nitric oxide and bradykinin [30] .…”

Section: Discussionmentioning

confidence: 99%

Endogenous Hypertriglyceridemia Intensifies the Course of Cerulein-Induced Pancreatitis in Rat: Relation with Changes in the VLDL Composition

Ponzo¹,

Schreier

Resnik³

et al. 2006

Ann Nutr Metab

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Aims: To study if the course of cerulein-induced pancreatitis in rats changes in a state of triglyceride-rich lipoprotein metabolism alteration. Methods: Two groups of rats received control diet during a 90-day period (A) and sucrose-rich diet to induce endogenous hypertriglyceridemia (B). Subgroups A2 and B2 received i.p. 45 µg cerulein/kg body weight (to induce acute pancreatitis). Histological examination of pancreas tissue, serum pancreatic lipase, lipoprotein profile and VLDL chemical composition were assessed. Then, pancreatic lipase hydrolytic activity on VLDL-triglycerides was evaluated in vitro. Results: Cellular vacuolization was observed in all of the cerulean-injected rats, but only in subgroup B2 fat necrosis was present. Serum triglycerides were higher in subgroup B1 than in subgroup A1 (mean ± SEM, mg/dl 123,77 ± 25.7 vs. 65.8 ± 7, p < 0.01). Triglycerides from rats fed with sucrose-rich diet, decreased after cerulein-induced pancreatitis (80.38 ± 11.3 vs. 123,77 ± 25.7, p < 0.02). Moreover, the endogenous hypertriglyceridemic rats showed an increment of VLDL triglyceride content, which decreased when rats were injected with cerulein. A negative correlation was found between VLDL-triglyceride content and serum pancreatic lipase activity (r = 0.58, p < 0.02). The in vitro assay showed a decrease in VLDL-triglyceride content post incubation with pancreatic lipase enriched serum (mean ± SD: 59.2 ± 27.7%, p < 0.01). Conclusions: The endogenous hypertriglyceridemia intensifies the course of cerulein-induced pancreatitis and it could be related to the decrease in VLDL-triglycerides as a consequence of pancreatic lipase hydrolytic activity.

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Section: Discussionmentioning

confidence: 99%

Endogenous Hypertriglyceridemia Intensifies the Course of Cerulein-Induced Pancreatitis in Rat: Relation with Changes in the VLDL Composition

Ponzo¹,

Schreier

Resnik³

et al. 2006

Ann Nutr Metab

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“…Indeed, studies in rats showed that heparin administration in experimental acute pancreatitis improved both pancreatic microcirculation and the inflammatory response (reduction in leukocyte-endothelium interaction). 74,75 Moreover, anticoagulant treatment with heparin seems to reduce platelet entrapment in the lungs during acute pancreatitis. 76 On the other hand, the role of heparin in postYendoscopic retrograde cholangiopancreatography (ERCP) pancreatitis is still unclear.…”

Section: Pathophysiology Of Hemostatic Disorders In Acute Pancreatitimentioning

confidence: 99%

Coagulation, Platelets, and Acute Pancreatitis

Kakafika

Papadopoulos²,

Mimidis³

et al. 2007

Pancreas

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Acute pancreatitis generates a complex cascade of immunological events that affect the pathogenesis and the progression of this disease. Several inflammatory mediators seem to play a critical role in the pathogenesis of pancreatitis and the subsequent inflammatory response. In turn, these mediators can influence hemostasis. Coagulation abnormalities occur in acute pancreatitis and are related to its severity. The contribution of blood platelets in the disturbed hemostasis in acute pancreatitis, although extensively studied, remains obscure. This article reviews the local and systemic implications of hemostatic abnormalities during acute pancreatitis. Furthermore, we discuss the prognostic value and the potential therapeutic implications of platelet activation and other hemostatic variables.

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“…to affect the development of microcirculatory disturbances in experimental acute pancreatitis [4,7,12,13,15,19,31,36,37].…”

Section: Discussionmentioning

confidence: 99%

“…Although the role of microcirculation disturbances in the pathogenesis of acute pancreatitis is well known [5,13,16,18,23,32,41,44], there are only limited number of studies dealing with general hemorheological or micro-rheological changes [e.g., 4,6,7,20]. In the development of microcirculatory disturbance in severe acute pancreatitis numbers of cytokines, inflammatory mediators and reactive oxygen species play role [4,8,12,14,21,23,26,32,34,36,37,42,43].…”

Section: Introductionmentioning

confidence: 99%

Effects of various drugs (flunixin, pentoxifylline, enoxaparin) modulating micro-rheological changes in cerulein-induced acute pancreatitis in the rat

Szentkereszty

Kotán

Kiss

et al. 2014

Clinical Hemorheology and Microcirculation

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Previously we have investigated the cerulein-induced acute pancreatitis and provided data on its micro-rheological impact in the rat. We hypothesized that non-steroid anti-inflammatory agent flunixin, the xanthine-derivate pentoxifylline and the low molecular weight heparin enoxaparin may have various beneficial effects improving microcirculatory and rheological parameters. In female rats, under general anesthesia, 10 g/kg cerulein s.c. was administered and 2 hours afterwards microcirculation was tested by laser Doppler flowmetry on the tongue and after performing laparotomy on the small intestine, liver and pancreas prior to terminal blood sampling. From blood samples hematological parameters, blood pH, lactate concentration, erythrocyte deformability, osmoscan parameters and erythrocyte aggregation were tested. Compared to normal control in acute pancreatitis group we found severe deterioration in tissue microcirculation together with impaired erythrocyte deformability and enhanced aggregation, accompanied by acidic pH and increasing lactate concentration. Improvement was found when using flunixin (s.c.), pentoxifylline (i.p.) or enoxaparin (s.c.). These drugs could partly improve the blood flux on the surface of the investigated organs, and the flunixin had the most expressed improving effects on micro-rheological parameters. Surprisingly, the improving effect of pentoxifylline on micro-rheological parameters was not obvious (red blood cell deformability did not improved better than in the other treated groups), however, microcirculatory parameters improved.

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Heparin improves organ microcirculatory disturbances in caerulein-induced acute pancreatitis in rats

Abstract: Heparin administration has a positive influence on organ microcirculatory disturbances accompanying experimental Cn-induced acute pancreatitis.

Cited by 17 publications

References 30 publications

Endogenous Hypertriglyceridemia Intensifies the Course of Cerulein-Induced Pancreatitis in Rat: Relation with Changes in the VLDL Composition

Endogenous Hypertriglyceridemia Intensifies the Course of Cerulein-Induced Pancreatitis in Rat: Relation with Changes in the VLDL Composition

Coagulation, Platelets, and Acute Pancreatitis

Effects of various drugs (flunixin, pentoxifylline, enoxaparin) modulating micro-rheological changes in cerulein-induced acute pancreatitis in the rat

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