Heparin-functionalized thermoresponsive surface

Terminus‐Selective Covalent Immobilization of Heparin on a Thermoresponsive Surface Using Click Chemistry for Efficient Binding of Basic Fibroblast Growth Factor

“…The introduction of heparin with condensation onto poly(IPAAm-co-CIPAAm)-grafted surfaces with >2 mol% CIPAAm greatly reduced the adhesiveness of mouse fibroblasts. [19] In contrast, the introduction of azide groups onto poly(IPAAm-co-CIPAAm)-grafted surfaces was considered to be more efficient than the direct introduction of heparin because of the reduced steric hindrance of 3-azidopropylamine toward the grafted chains of poly(IPAAm-co-CIPAAm). The introduction of 3-azidopropylamine did not interfere with the adhesiveness of the cells, even if the unreacted azide groups remained after CuAAC.…”

“…Immobilization of Heparin on Thermoresponsive Surfaces by Condensation and CuAAC: IC1 4-well plate and 35 mm dish were prepared as previously described. [19] Briefly, a 55% wt monomer solution (6 and 28 μL) containing IPAAm and CIPAAm (99:1 molar ratio) in 2-propanol was spread on 4-well plate (1.9 cm 2 ) and 35 mm dish (8.8 cm 2 ), respectively. Next, the spread monomer solution was irradiated with an electron beam from an areabeam electron-processing system (Curetron EBC-200-AA2; Nissin High Voltage, Kyoto, Japan) to promote polymerization and covalent grafting of the polymer to the surface.…”

“…[16][17][18] We have previously prepared bFGFbound thermoresponsive surfaces by tethering heparin through a condensation reaction with thermoresponsive copolymergrafted surfaces containing 1 mol% active ester-formed 2carboxyisopropylacrylamide (CIPAAm). [19] However, it was also found that surfaces containing more CIPAAm became more hydrophilic, resulting in a significant decrease in the adhesiveness of cells. Another potential concern is that the condensation reaction may induce multi-point immobilization of heparin on the surface because heparin has multiple reaction points (e.g., amino and/or hydroxyl groups) within a single molecule.…”

A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets

“…In order to harvest cultured cells nonenzymatically with maintaining cellular functions, our laboratory reported a new type of stimuli-responsive surface that was co-immobilized with ECMs and growth factors [5]. Recently, we designed thermoresponsive poly( N -isopropylacrylamide) (PIPAAm)-grafted surfaces modified with heparin molecules for switching of sustained stimulation and detachment of cultured cells [6], [7]. Heparin has affinity interactions with bioactive molecules such as growth factors, ECMs, and protease [8].…”