Heparin fails to inhibit the leukocyte recruitment for an extended time following inflammatory stimulus

Arimateia, Dayse Santos; Brito, Adriana da Silva; Azevedo, Fernanda Marques de; Andrade, Giulianna P.V.; Chavante, Suely F.

doi:10.3109/13880209.2014.910534

Cited by 4 publications

(2 citation statements)

References 38 publications

(35 reference statements)

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“…Therefore, it must be hypothesized that different antiplatelet/anticoagulant agents may produce different effects on the periodontal breakdown. The literature also shows that heparin effect on inflammatory stimulus is dependent on its dosage and the duration of the inflammation (16), which partially explain the results found in the present study. In this sense, therapeutic doses of LMWH may be expected to be safely used in patients with periodontitis.…”

Section: Discussionsupporting

confidence: 88%

Effects of Low Molecular Weight Heparin on Alveolar Bone Loss in Wistar Rats

et al. 2019

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This study aimed to assess the effects of low molecular weight heparin (LMWH) on alveolar bone loss (ABL), blood count, and counting of megakaryocytes and adipocytes in male Wistar rats. Forty male 60-day Wistar rats were randomly divided into four groups: Control (C), Periodontal Disease (PD), Heparin (Hp) and Heparin + Periodontal Disease (Hp+PD). LMWH was applied for 60 days at doses of 1 ml/kg/day. Blood samples were collected at baseline, 30 and 60. On day-49, PD and Hp+PD groups were subjected to ligature-induced periodontitis around second upper right molar. The left side was assessed as spontaneous alveolar bone loss. Mean ABL in the side with ligature showed significantly different between C (0.35±0.07 mm) and Hp+DP (0.49±0.09 mm) groups (p<0.001), between PD (0.55±0.11 mm) and Hp (0.32±0.06 mm) groups (p<0.001) and between Hp and Hp+DP groups (p<0.001). No significant differences were found among groups for ABL in the side without ligature. Animal weight, food intake, and water consumption showed no statistically significant difference among groups. Megakaryocytes and adipocytes were counted using optical microscopy and no statistically significant differences were found. Within-groups, there were an increase and a decrease, respectively, in the counting of lymphocytes (p=0.005 for C and p=0.009 for Hp+PD groups only) and leukocytes (p=0.003 for C, p=0.001 for PD, p=0.002 for Hp, and p<0.001 for Hp+PD groups). There was no decrease in the number of platelets in the three collection periods. LMWH was not able to affect ABL, but it may change the blood counting, especially increasing lymphocytes.

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Section: Discussionsupporting

confidence: 88%

Effects of Low Molecular Weight Heparin on Alveolar Bone Loss in Wistar Rats

et al. 2019

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“…The administration of a GAG, usually heparin, is a method that has been employed in pre-clinical models to modulate inflammation, and is thought to act through disruption of pre-formed chemokine gradients present on cell surface GAGs. Heparin in various forms inhibits leukocyte recruitment to mouse models of arthritis, traumatic brain injury and lipopolysaccharide (LPS) treatment [ 106 , 107 , 108 ], although its effectiveness depends upon the dose given and the duration of inflammation [ 109 ]. These studies show potential role of GAG mimetics on chemokine-mediated immunomodulation when administered, either local or systemically, however it should be noted that administered heparin is likely to interact with all cytokines due to its highly negative charge, and a more chemokine-specific gradient disruption method could be more beneficial.…”

Section: Gags Nitration and Cxcl8 Functionmentioning

confidence: 99%

Regulation of Chemokine Function: The Roles of GAG-Binding and Post-Translational Nitration

Thompson

Martínez-Burgo

Sepuru

et al. 2017

IJMS

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The primary function of chemokines is to direct the migration of leukocytes to the site of injury during inflammation. The effects of chemokines are modulated by several means, including binding to G-protein coupled receptors (GPCRs), binding to glycosaminoglycans (GAGs), and through post-translational modifications (PTMs). GAGs, present on cell surfaces, bind chemokines released in response to injury. Chemokines bind leukocytes via their GPCRs, which directs migration and contributes to local inflammation. Studies have shown that GAGs or GAG-binding peptides can be used to interfere with chemokine binding and reduce leukocyte recruitment. Post-translational modifications of chemokines, such as nitration, which occurs due to the production of reactive species during oxidative stress, can also alter their biological activity. This review describes the regulation of chemokine function by GAG-binding ability and by post-translational nitration. These are both aspects of chemokine biology that could be targeted if the therapeutic potential of chemokines, like CXCL8, to modulate inflammation is to be realised.

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Expression of DEL-1 in alveolar epithelial cells prevents lipopolysaccharide-induced inflammation, oxidative stress, and eosinophil recruitment in acute lung injury

Zeng

Ren

2022

International Immunopharmacology

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Heparin fails to inhibit the leukocyte recruitment for an extended time following inflammatory stimulus

Abstract: These findings show that the heparin effect on leukocyte recruitment varies depending on its dosage and the duration of the inflammation.

Cited by 4 publications

References 38 publications

Effects of Low Molecular Weight Heparin on Alveolar Bone Loss in Wistar Rats

Effects of Low Molecular Weight Heparin on Alveolar Bone Loss in Wistar Rats

Regulation of Chemokine Function: The Roles of GAG-Binding and Post-Translational Nitration

Expression of DEL-1 in alveolar epithelial cells prevents lipopolysaccharide-induced inflammation, oxidative stress, and eosinophil recruitment in acute lung injury

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