2011
DOI: 10.1016/j.neulet.2011.04.072
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Heparin enhances the cell-protein misfolding cyclic amplification efficiency of variant Creutzfeldt–Jakob disease

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Cited by 45 publications
(43 citation statements)
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“…This result suggests that HS and HP perform the same function as nucleic acids in prion replication in vitro. HS and HP have been known to promote PrP conversion into a PrP Sc -like form in in vitro cell-free conversion assays (10,12); however, we have demonstrated for the first time that HS and HP are critical for the replication of infectious prions. Furthermore, our study revealed that the HS and HP concentrations required to induce the conversion differed between prion strains.…”
Section: Discussionmentioning
confidence: 60%
See 1 more Smart Citation
“…This result suggests that HS and HP perform the same function as nucleic acids in prion replication in vitro. HS and HP have been known to promote PrP conversion into a PrP Sc -like form in in vitro cell-free conversion assays (10,12); however, we have demonstrated for the first time that HS and HP are critical for the replication of infectious prions. Furthermore, our study revealed that the HS and HP concentrations required to induce the conversion differed between prion strains.…”
Section: Discussionmentioning
confidence: 60%
“…Sulfate Groups on HP Are Critical for Bac-PrP res Amplification-GAG length and degree of sulfation were previously reported to affect the conversion of PrP C or recPrP into PrP Sc (9,10,(35)(36)(37)(38). Therefore, to examine the relative effects of GAG length and sulfation degree on Bac-PrP conversion into Bac-PrP res , we added chemically modified HPs to iPMCA reaction mixtures under nucleic acid-depleted conditions (Fig.…”
Section: Gags Did Not Affect Bac-prp Conversion Into a Pk-resistant Fmentioning
confidence: 99%
“…To assess the effect of residue substitutions on PrP C to PrP Sc conversion, we performed seeded conversion experiments using PrP C derived from cell lysates as a substrate and PrP Sc from prion-infected brain homogenate as a seed as described recently (22,41,42). PrP C -deficient RK13 cells (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Alternatively, changes in the intracellular distribution or trafficking kinetics of PrP Sc and endogenous GAGs could contribute to the antiprion activity of GAG modulators. In vitro studies demonstrate a direct effect of GAGs and GAG analogues on PrP Sc amplification, potentially by providing a scaffold for PrP C -PrP Sc interactions and conversion (13,45,66). The observed reduction in endogenous GAGs could thus directly affect the conversion process and thereby abrogate prion propagation.…”
Section: Discussionmentioning
confidence: 99%