Heparin conjugated polymeric micelle for long-term delivery of basic fibroblast growth factor

Lee, Jung Seok; Go, Dong Hyun; Bae, Jin Woo; Lee, Seung Jin; Парк, Ки Донг

doi:10.1016/j.jconrel.2006.11.004

Cited by 86 publications

(59 citation statements)

References 35 publications

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“…However, the LHSA1 and LHSA3 conjugates did not possess the hydrophobicity necessary for the formation of stable nanoparticles due to the low substitution ratio of SA. Additionally, the CMC value of the LHSA5 conjugate was significantly lower (44 μg mL −1 ) than those of other lowmolecular-weight surfactants or amphiphilic polymers, 49,50 indicating that it can form stable nanoparticle structures, even in the presence of the reduced polymer concentrations after dilution with body fluids.…”

Section: Discussionmentioning

confidence: 93%

Preparation and characterization of self-assembled nanoparticles based on low-molecular-weight heparin and stearylamine conjugates for controlled delivery of docetaxel

Kim¹,

Termsarasab²,

Cho³

et al. 2014

IJN

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Low-molecular-weight heparin (LMWH)–stearylamine (SA) conjugates (LHSA)-based self-assembled nanoparticles were prepared for intravenous delivery of docetaxel (DCT). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide and N -hydroxysuccinimide were used as coupling agents for synthesis of LHSA conjugates. The physicochemical properties, in vitro antitumor efficacy, in vitro cellular uptake efficiency, in vivo antitumor efficacy, and in vivo pharmacokinetics of LHSA nanoparticles were investigated. The LHSA nanoparticles exhibited a spherical shape with a mean diameter of 140–180 nm and a negative surface charge. According to in vitro release and in vivo pharmacokinetic test results, the docetaxel-loaded LHSA5 (LMWH:SA =1:5) nanoparticles exhibited sustained drug release profiles. The blank LHSA nanoparticles demonstrated only an insignificant cytotoxicity in MCF-7 and MDAMB 231 human breast cancer cells; additionally, higher cellular uptake of coumarin 6 (C6) in MCF-7 and MDAMB 231 cells was observed in the LHSA5 nanoparticles group than that in the C6 solution group. The in vivo tumor growth inhibition efficacy of docetaxel-loaded LHSA5 nanoparticles was also significantly higher than the Taxotere ® -treated group in the MDAMB 231 tumor-xenografted mouse model. These results indicated that the LHSA5-based nanoparticles could be a promising anticancer drug delivery system.

show abstract

Section: Discussionmentioning

confidence: 93%

Preparation and characterization of self-assembled nanoparticles based on low-molecular-weight heparin and stearylamine conjugates for controlled delivery of docetaxel

Kim¹,

Termsarasab²,

Cho³

et al. 2014

IJN

View full text Add to dashboard Cite

show abstract

“…Changes in anisotropy of fluorescent probes have also been associated with the onset of micellization [163,164]. Pyrene has been used by many research groups for CMC determination of the polymeric micelles; few examples include micelles formed from methoxy PEG-PCL, [165] [169], and Tetronic-PCL-heparin [170].…”

Section: Fluorescence Probementioning

confidence: 99%

Polymeric micelles: authoritative aspects for drug delivery

Kulthe

Choudhari

Inamdar

et al. 2012

Designed Monomers and Polymers

187

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“…One of the primary goals in this study was to solubilize PTX by means of hydrotropic micelles without using any organic solvent. Typical method to generate micelles and to load a poorly water-soluble drug into the micelles has used organic solvent, which might be removed by dialysis [10,30] or evaporation methods [31,32]. Even in the previous study on the hydrotropic PEG-b-P(2-VBODENA) polymer micelle, the very high loading content could be obtained via a dialysis method, but with use of acetonitrile as a solvent to dissolve PTX and the polymer.…”

Section: Property Of Paclitaxel-containing Hydrotropic Micellesmentioning

confidence: 99%

A new hydrotropic block copolymer micelle system for aqueous solubilization of paclitaxel

Huh

Min

Lee

et al. 2008

Journal of Controlled Release

106

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Paclitaxel (PTX), a potent anti-cancer drug, is poorly soluble in water, and this has been a major limitation in developing patient friendly formulations for clinical applications. Recent studies on polymeric micelles, especially hydrotropic polymer micelles, have suggested an alternative formulation of PTX based on their high loading capacity and physical stability in aqueous media. The present study aims at aqueous solubilization of PTX in polymer micelles without using any organic solvents that is usually required for solubilization in polymer micelles. Poly(ethylene glycol) was used as a hydrophilic block and, as a hydrotropic block, poly(4-(2-vinylbenzyloxy-Npicolylnicotinamide)) (P(2-VBOPNA)) was synthesized by atom transfer radical polymerization. The hydrotropic block copolymers did not form a micellar structure at pH 2 or below due to protonation of PNA groups, but the aqueous solubility of PTX increased significantly by the hydrotropic activity of P(2-VBOPNA). At pH values higher than 2, the PTX solubility increased even further due to deprotonation of 2-VBOPNA, leading to effective polymer micellization. A longer hydrotropic block resulted in higher aqueous PTX solubility, and slightly slower release rate from the micelles. The hydrotropic block copolymers synthesized in this study are able to form PTXloaded polymeric micelles in aqueous solution without using any organic solvents.

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Heparin conjugated polymeric micelle for long-term delivery of basic fibroblast growth factor

Cited by 86 publications

References 35 publications

Preparation and characterization of self-assembled nanoparticles based on low-molecular-weight heparin and stearylamine conjugates for controlled delivery of docetaxel

Preparation and characterization of self-assembled nanoparticles based on low-molecular-weight heparin and stearylamine conjugates for controlled delivery of docetaxel

Polymeric micelles: authoritative aspects for drug delivery

A new hydrotropic block copolymer micelle system for aqueous solubilization of paclitaxel

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