Heparin-binding epidermal growth factor-like growth factor (HB-EGF) induction on Snail expression during mouse decidualization

Zhang, Xiuhong; Liang, Xuan; Wang, Tong-Song; Liang, Xiao-Huan; Zuo, Ru-Juan; Deng, Wenbo; Zhang, Zhirong; Qin, Funiu; Zhao, Zhen-Ao; Yang, Zeng‐Ming

doi:10.1016/j.mce.2013.08.011

Cited by 17 publications

(17 citation statements)

References 45 publications

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“…4) or Rev-erb␣-siRNA (data not shown). Therefore, during decidualization, it is considered that Ptgs2 induction requires the intracellular downregulation of Rev-erb␣ as well as the extracellular inducible signals previously reported, such as those by cytokines, ovarian steroid hormones, growth factors, and PGs (11,28,29,58). In Fig.…”

Section: Discussionmentioning

confidence: 93%

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Isayama

Zhao

Chen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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The rhythmic expression of clock genes in the uterus is attenuated during decidualization. This study focused on Ptgs2, which is essential for decidualization, as a putative clock-controlled gene, and aimed to reveal the functions of clock genes in relation to Ptgs2 during decidualization. We compared the transcript levels of clock genes in the rat uterus on days 4.5 (D4.5) and 6.5 of pregnancy. The transcript levels of clock genes (Per2, Bmal1, Rorα, and Rev-erbα) had decreased at implantation sites on day 6.5 (D6.5e) compared with those on D4.5, whereas Ptgs2 transcripts had increased on D6.5e. Similar observations of Rev-erbα and Ptgs2 were also obtained in the endometrium on D6.5e by immunohistochemistry. In the decidual cells induced by medroxyprogesterone and 2-O-dibutyryl-cAMP, the rhythmic expression levels of clock genes were attenuated, whereas Ptgs2 transcription was induced. These results indicate that decidualization causes the attenuation of clock genes and the induction of Ptgs2. Furthermore, in the experiment of Bmal1 siRNA, the rhythmic expression of clock genes and Ptgs2 was attenuated by the siRNA. Transcript levels of Ptgs2 and prostaglandin (PG)E₂ production were increased by treatment with the Rev-erbα antagonist, suggesting the contribution of the nuclear receptor Rev-erbα to Ptgs2 expression. Moreover, Rev-erbα knockdown enhanced the induction of Ptgs2 transcription and PGE₂ production by forskolin. Chromatin immunoprecipitation-PCR analysis revealed that Rev-erbα could directly bind to a proximal RORE site of Ptgs2. Collectively, this study demonstrates that the attenuation of the circadian clock, especially its core component Rev-erbα, contributes to the induction of Ptgs2 during decidualization.

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Section: Discussionmentioning

confidence: 93%

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Isayama

Zhao

Chen

et al. 2015

American Journal of Physiology-Endocrinology and Metabolism

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“…PR could be involved in regulating Snail, a transcription repressor which has recently been identified to play a central role in the epithelial-mesenchymal transition in which its expression has been reported to be induced by HB-EGF via EGFR-ERK-STAT3 signaling pathway [99]. HOXA-10, an abdominal-like homeobox gene reported to be involved in the decidualization process, could also be influenced by PR where loss of Hoxa-10 function in mice has been reported to result in infertility [100].…”

Section: Role Of Pgs In Decidualizationmentioning

confidence: 99%

Diverse Roles of Prostaglandins in Blastocyst Implantation

Salleh

2014

The Scientific World Journal

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Prostaglandins (PGs), derivatives of arachidonic acid, play an indispensable role in embryo implantation. PGs have been reported to participate in the increase in vascular permeability, stromal decidualization, blastocyst growth and development, leukocyte recruitment, embryo transport, trophoblast invasion, and extracellular matrix remodeling during implantation. Deranged PGs syntheses and actions will result in implantation failure. This review summarizes up-to-date literatures on the role of PGs in blastocyst implantation which could provide a broad perspective to guide further research in this field.

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“…Human trophoblast differentiation is regulated by redox balance because early trophoblast differentiation or invasion alterations lead to pregnancy complications such as an abnormal placentation. Snail expression is sensitive to hypoxia, either directly through activation of the HIF-1α responsive element on its promoter or by epidermal growth factor (EGF), transforming growth factor (TGF), α, β, and tumoral necrosis factor (TNFα) mediators activated by hypoxic signals [2][3][4]. Snail upregulation by HIF-1α in cultured human first trimester villous explants reduces E-cadherin expression and activates an invasion program [5].…”

Section: Introductionmentioning

confidence: 99%

Iodine Affects Differentiation and Migration Process in Trophoblastic Cells

Vidal

Rodríguez

Arroyo-Helguera

2015

Biol Trace Elem Res

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Iodine deficiency is associated with oxidative stress increase and preeclampsia during gestation, suggesting that iodine concentration plays an important role in the normal placenta physiology. The question raised is to analyze the effect of iodine deficiency on oxidative stress, viability, differentiation, and migration process and changes in the expression of differentiation and migration markers. Iodine deprivation was done using potassium perchlorate (KCLO4) to block sodium iodide symporter (NIS) transporter and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid DIDS to inhibit pendrine (PEN) transport for 3-48 h. Then trophoblast cells were treated with low iodine doses of 5-500 μM and high iodine doses of 100-5000 μM. Oxidative stress, viability, and human chorionic gonadotropin (hGC) were measured by colorimetric methods. Migration throphoblast cells were evaluated by both wound healing and Boyden chamber assays. Changes in mRNA expression were analyzed by real-time RT-PCR. Iodine deprivation induces a significant increase of reactive oxygen species (ROS), viability, and migration process vs control cells. We found a significant overregulation in the mRNA's peroxisome proliferator-activated receptor (PPAR-gamma), Snail, and matrix metalloproteinase-9 (MMP-9) mRNA's in cells deprived of iodine, as well as a down glial cell missing-1 (GCM-1) regulation, hGC, pregnancy-associated plasma protein-A (PAPP-A), and E-cadherin mRNA expression. The expression of hypoxic induction factor alpha (HIFα) mRNA does not change with iodine deprivation. In cells deprived of iodine, supplementing low iodine doses (5-500 μM) does not induce any significant changes in viability. However, ROS and migration process were decreased, although we found an increased human chorionic gonadotropin (hCG) secretion as a differentiation marker. In addition, we found that PPAR-gamma, Snail, and MPP-9 mRNAs expression are downregulated with low iodine doses, in contrast with GCM-1, PAPP-A, hGC, and E-cadherin that increase their expression vs cells deprived of iodine. High iodine doses (1000-5000 μM) have shown cytotoxic effects. Based on our results, iodine is important for keeping the proliferation/differentiation balance in the placenta.

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Heparin-binding epidermal growth factor-like growth factor (HB-EGF) induction on Snail expression during mouse decidualization

Cited by 17 publications

References 45 publications

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells

Diverse Roles of Prostaglandins in Blastocyst Implantation

Iodine Affects Differentiation and Migration Process in Trophoblastic Cells

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