Heparin-binding EGF-like growth factor is present in human amniotic fluid and breast milk

Michalsky, Marc P.; Lara-Marquez, Maria Luz; Lu, Chun; Besner, Gail E.

doi:10.1053/jpsu.2002.29415

Cited by 53 publications

(40 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, they used a radioreceptor technique, which is based on a competitive binding of ligands to the EGF-R. This method simultaneously detects several members of the EGF-like peptide family, such as EGF, TGF-␣, heparinbinding EGF, and betacellulin, all reported to be found in milk (12,14,18,27). In the present study, we used a peptidespecific and quantitative RIA, with no cross-reactivity among members of the EGF-like family of peptides.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Increased Epidermal Growth Factor Levels in Human Milk of Mothers with Extremely Premature Infants

et al. 2003

View full text Add to dashboard Cite

Maternal milk is the major source of nutrients and growthpromoting substances in the first weeks of life for the majority of neonates. Epidermal growth factor (EGF) and transforming growth factor-␣ (TGF-␣) are trophic peptides present in human milk with significant healing effects on injured gastrointestinal mucosa. Decreasing gestational age of neonates is associated with higher risk of developing gastrointestinal disorders, and human milk provides better protection against these diseases compared with formula. The aim of this study was to evaluate the concentrations of EGF and TGF-␣ in human milk collected from mothers with infants born: extremely preterm, preterm, and full term. Milk samples were collected at the end of first, second, and fourth week postpartum from each mother of infants born in one of the three gestational age groups: extremely preterm (23-27 wk, n ϭ 16), preterm (32-36 wk, n ϭ 16), and full term (38 -42 wk, n ϭ 15). Milk concentrations of EGF and TGF-␣ were quantified with a homologous RIA in the milk aqueous fraction. Concentrations of EGF in human milk from the extremely preterm group (23-27 wk) were significantly higher compared with values from the preterm and full-term groups throughout the first month of lactation. A similar pattern was observed with human milk TGF-␣; however, milk TGF-␣ levels were lower than EGF.In conclusion, we have found higher concentrations of EGF and TGF-␣ in human milk of mothers with extremely preterm babies. These data may indicate the potential importance of milk-borne EGF and TGF-␣ for the development of extremely premature infants. (Pediatr Res 54: 15-19, 2003) Abbreviations EGF, epidermal growth factor EGF-R, epidermal growth factor receptor EPT, extremely preterm FT, full term PT, preterm NEC, necrotizing enterocolitis TGF-␣, transforming growth factor-␣ Human milk is a unique and well-balanced source of nutrition for the newborn. Milk contains not only major nutrients (proteins, carbohydrates, and lipids) but also a variety of components such as minerals, vitamins, enzymes, hormones, growth factors, and immunoglobulins important for growth and healthy development during the neonatal period (1-3). As advances in neonatal care have significantly increased, the survival of extremely prematurely born neonates have increased (4). Yet these infants have the highest incidence of morbid events, such as NEC (5). Questions have arisen about the most suitable and relevant nutritional support for these immature infants (6, 7).Within the last three decades, a large number of biologically active peptides have been identified in human milk (8 -10), and the list is continuously growing (11-13). Among these, EGF is one of the major peptide growth factors present both in colostrum and human milk (14 -17). Human milk EGF levels are highest in the first days after parturition and then gradually decrease during the first 2 wk of lactation (17). Another structural homolog of EGF, TGF-␣ is also present in human colostrum and milk (15, 18), but at much lower concentrations ...

show abstract

Section: Discussionmentioning

confidence: 99%

“…Within the last three decades, a large number of biologically active peptides have been identified in human milk (8 -10), and the list is continuously growing (11)(12)(13). Among these, EGF is one of the major peptide growth factors present both in colostrum and human milk (14 -17).…”

mentioning

confidence: 99%

Increased Epidermal Growth Factor Levels in Human Milk of Mothers with Extremely Premature Infants

et al. 2003

View full text Add to dashboard Cite

show abstract

“…HER2/neu is one of four identifi ed EGF receptors so far (besides to EGFR/HER1, cerb-B3 and c-erb-B4). Unlike other EGF receptors which bind numerous paracrine ligands like EGF, transforming growth factor alpha (TGFα), hergulin, heparin-binding EGF-like growth factor, beta-cellulin and epiregulin, HER2/ neu has no specifi c ligand; it serves as a modulator of growth and division signal received by EGFR [11,12]. When overexpressed on the cell membrane, it can spontaneously form homodimers which have strong signal activity without binding specifi c ligands by EGFR and, thus, lead to autonomous cell growth stimulation [13].…”

Section: Introductionmentioning

confidence: 99%

HER2/ECD Serumwerte bei Stadium I und II Brustkrebs – brauchen wir niedrigere Grenzwerte?

Badzek

Kelović

Pleština

et al. 2011

Wien Klin Wochenschr

View full text Add to dashboard Cite

41 patients with stage I and II breast cancer and 52 healthy controls were included into the study. HER2/ECD was determined before surgery and correlated with HER2/neu overexpression, Ki67, hormone receptor status and disease stage, and compared with value in healthy controls. Mean serum HER2/ECD concentration in patients was 8.62 ng/ml and 5.78 ng/ml in controls, and the difference was statistically significant (p = 0.000061). The best diagnostic cut-off value was 7.7 ng/ml, with 76.92% sensitivity and 72.92% specificity. Positive predictive value of the test was 69.77% and negative predictive value was 79.55%, with 74.71% of patients correctly classified. Serum HER2/ECD correlated with hormone receptors status, and no correlation with histological overexpression has been observed. CONCLUSION. Serum HER2/ECD concentration of ≥7.7 ng/ml has possible diagnostic value in stage I and II breast cancer. It should not be used as a determinant of HER2 positivity. Prognostic significance of HER2/ECD in early breast cancer, its correlation with hormone receptor status, and interconnection between hormone receptors and HER2 receptor signaling should be further analyzed, since it may have therapeutic implications.

show abstract

“…HB-EGF is also found in amniotic fluid and in human milk, and its expression is elevated in response to injury, such as tissue damage, hypoxia, and stress [64]. In vitro and in vivo studies of intestinal anoxia/reoxygenation and ischemia/reperfusion show that HB-EGF increases after injury and improves epithelial restitution by acting on EGFR [65].…”

Section: Growth Factorsmentioning

confidence: 99%

Pathogenesis of neonatal necrotizing enterocolitis

2015

View full text Add to dashboard Cite

Although necrotizing enterocolitis (NEC) is the most lethal gastrointestinal disease in the neonatal population, its pathogenesis is poorly understood. Risk factors include prematurity, bacterial colonization, and formula feeding. This review examines how mucosal injury permits opportunistic pathogens to breach the gut barrier and incite an inflammatory response that leads to sustained overproduction of mediators such as nitric oxide and its potent adduct, peroxynitrite. These mediators not only exacerbate the initial mucosal injury, but they also suppress the intestinal repair mechanisms, which further compromises the gut barrier and culminates in bacterial translocation, sepsis, and full-blown NEC.

show abstract

Heparin-binding EGF-like growth factor is present in human amniotic fluid and breast milk

Cited by 53 publications

References 21 publications

Increased Epidermal Growth Factor Levels in Human Milk of Mothers with Extremely Premature Infants

Increased Epidermal Growth Factor Levels in Human Milk of Mothers with Extremely Premature Infants

HER2/ECD Serumwerte bei Stadium I und II Brustkrebs – brauchen wir niedrigere Grenzwerte?

Pathogenesis of neonatal necrotizing enterocolitis

Contact Info

Product

Resources

About