Heparin and Protease Inhibitors in the Prevention of Experimental Acute Pancreatic Necrosis in Dogs

Gabryelewicz, A; Niewiarowski, S; Prokopowicz, J; Chlebowski, J

doi:10.1159/000196915

Cited by 25 publications

(13 citation statements)

References 13 publications

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“…Previous experimental and clinical studies have shown the protective and therapeutic effect of heparin in acute pancreatitis [11,12,13,14,15,16]. Treatment with heparin has been also found to be effective in the prevention of pancreatitis-evoked encephalopathy [17].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Low Doses of Acenocoumarol in the Course of Ischemia/Reperfusion-Induced Acute Pancreatitis in Rats

Warzecha

Sendur

Ceranowicz

et al. 2017

IJMS

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Intravascular activation of coagulation is observed in acute pancreatitis and is related to the severity of this inflammation. The aim of our study was to evaluate the impact of acenocoumarol therapy on the course of acute pancreatitis induced in male rats by pancreatic ischemia followed by reperfusion. Acenocoumarol at a dose of 50, 100, or 150 µg/kg/dose was administered intragastrically once a day, starting the first dose 24 h after the initiation of pancreatic reperfusion. Results: Histological examination showed that treatment with acenocoumarol reduces pancreatic edema, necrosis, and hemorrhages in rats with pancreatitis. Moreover, the administration of acenocoumarol decreased pancreatic inflammatory infiltration and vacuolization of pancreatic acinar cells. These findings were accompanied with a reduction in the serum activity of lipase and amylase, concentration of interleukin-1β, and plasma d-Dimer concentration. Moreover, the administration of acenocoumarol improved pancreatic blood flow and pancreatic DNA synthesis. Acenocoumarol given at a dose of 150 µg/kg/dose was the most effective in the treatment of early phase acute pancreatitis. However later, acenocoumarol given at the highest dose failed to exhibit any therapeutic effect; whereas lower doses of acenocoumarol were still effective in the treatment of acute pancreatitis. Conclusion: Treatment with acenocoumarol accelerates the recovery of ischemia/reperfusion-induced acute pancreatitis in rats.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Low Doses of Acenocoumarol in the Course of Ischemia/Reperfusion-Induced Acute Pancreatitis in Rats

Warzecha

Sendur

Ceranowicz

et al. 2017

IJMS

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“…Acute pancreatitis (AP) is associated with coagulative disorders [6,7,8,9]. The stage of these disorders depends on the severity of AP.…”

Section: Introductionmentioning

confidence: 99%

“…The stage of these disorders depends on the severity of AP. Mild AP is associated with scattered intravascular thrombosis, whereas severe AP may lead to the development of disseminated intravascular coagulation [6,7,8,9]. Measurement of disseminated intravascular coagulation parameters is useful in the assessment of AP severity and prediction for poor outcome of this disease at admission [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Animals studies have indicated that pretreatment with heparin inhibits the development of AP evoked by bile [9], taurocholate [10], cerulein [11] and pancreatic ischemia followed by reperfusion [12]. Administration of heparin after induction of AP has been shown to accelerate the recovery in acute pancreatitis evoked by cerulein [13] and pancreatic ischemia followed by reperfusion [12].…”

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Pretreatment with Acenocoumarol in Cerulein-Induced Acute Pancreatitis

Warzecha

Sendur

Ceranowicz

et al. 2016

IJMS

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Coagulation is recognized as a key player in inflammatory and autoimmune diseases. The aim of the current research was to examine the effect of pretreatment with acenocoumarol on the development of acute pancreatitis (AP) evoked by cerulein. Methods: AP was induced in rats by cerulein administered intraperitoneally. Acenocoumarol (50, 100 or 150 µg/kg/dose/day) or saline were given once daily for seven days before AP induction. Results: In rats with AP, pretreatment with acenocoumarol administered at the dose of 50 or 100 µg/kg/dose/day improved pancreatic histology, reducing the degree of edema and inflammatory infiltration, and vacuolization of acinar cells. Moreover, pretreatment with acenocoumarol given at the dose of 50 or 100 µg/kg/dose/day reduced the AP-evoked increase in pancreatic weight, serum activity of amylase and lipase, and serum concentration of pro-inflammatory interleukin-1β, as well as ameliorated pancreatic DNA synthesis and pancreatic blood flow. In contrast, acenocoumarol given at the dose of 150 μg/kg/dose did not exhibit any protective effect against cerulein-induced pancreatitis. Conclusion: Low doses of acenocoumarol, given before induction of AP by cerulein, inhibit the development of that inflammation.

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“…Pretreatment with heparin ceases the development of pancreatitis induced by cerulean, taurocholate and bile on various animal studies (Gabryelewicz et al, 1969). Moreover heparin restores the pancreatic function in cerulean induced pancreatitis if it is administered after the induction of pancreatitis (Dobosz et al, 1998;Qiu et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Spatholobus Suberectus Stem Extract Improves the Protective Effect of Heparin on Cerulein–induced Pancreatitis

Shao¹

2017

Afr J Tradit Complement Altern Med

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Background: The present study evaluates the effect of Spatholobus suberectus stem extract (SS) in the management of pancreatitis alone and in combination with heparin. Material and methods: Pancreatitis was induced pancreatitis by cerulean (50µg/kg, i.p.) five times at an interval of 1 h without any pretreatment of drug. Rats were treated with SS (100 and 200 mg/kg, p. o.) and heparin (150 U/kg, i.p.) alone and in combination for the duration of a week. Later pancreatic weight and blood flow was estimated and different biochemical parameters like concentration of D-dimer and Interleukin 1β (IL-1β) and activity of amylase and lipase were determined in blood of pancreatitis rats. Moreover effect of drug treatment on DNA synthesis and histopathology was also estimated on cerulean induced pancreatitis rats. Result: Results of this study suggest that treatment with SS alone and in combination with heparin significantly increase in prothrombin time and pancreatic blood flow than negative control group. There was significant decrease in concentration of IL-1β and D-dimer and activity of amylase and lipase in SS and heparin treated group than negative control group. Pancreatic DNA synthesis was also found to be reduced in SS and heparin alone and in combination treated group. Histopathology study also reveals that treatment with SS and heparin alone and in combination reduces edema, hemorrhages, leukocyte infiltration in the TS of pancreatic tissues. Conclusion: Present study concludes that treatment with SS alone effectively manages the pancreatitis by ceasing the inflammatory pathway and potentiates the effect of heparin in the management of pancreatitis.

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Heparin and Protease Inhibitors in the Prevention of Experimental Acute Pancreatic Necrosis in Dogs

Cited by 25 publications

References 13 publications

Therapeutic Effect of Low Doses of Acenocoumarol in the Course of Ischemia/Reperfusion-Induced Acute Pancreatitis in Rats

Therapeutic Effect of Low Doses of Acenocoumarol in the Course of Ischemia/Reperfusion-Induced Acute Pancreatitis in Rats

Protective Effect of Pretreatment with Acenocoumarol in Cerulein-Induced Acute Pancreatitis

Spatholobus Suberectus Stem Extract Improves the Protective Effect of Heparin on Cerulein–induced Pancreatitis

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