Hen egg immunoglobulin Y in colloidal gold agglutination assay: comparison with rabbit immunoglobulin G

Abstract

Conjugation of immunoglobulin G (IgG) from rabbit serum with colloidal gold for sol agglutination assay was conducted. Effects of various buffer systems on conjugation and stability of sols were studied. Colloidal gold assay for detection of human myoglobin and C-reactive protein was developed. It was shown that rabbit IgG had a poor stabilizing effect on colloidal gold sol and therefore that it cannot be effectively used in such assays. In contrast, hen egg immunoglobulin Y (IgY) had excellent stabilizing properties used in various buffer systems and in a wide range of concentrations. IgY was considered as a useful alternative for rabbit antibodies in gold sol agglutination assay.

Gold nanoparticles in biomedical applications: recent advances and perspectives

“…The authors employed an optimized variation of SPIA (by using larger gold particles and monoclonal antibodies to various antigenic sites) to detect human chorionic gonadotropin. 100 Subsequently, the assay was used for the immunoanalysis of Shistosoma 101 and Rubella 102 antigens; estimation of immunoglobulins, 103,104 thrombin (by using aptamers), 105 and glucose; 106 direct detection of cancerous cells; 107 detection of Leptospira cells in urine; 108 detection of Alzheimer's disease markers; 109 determination of protease activity; 110 and other purposes. The simultaneous use of antibody conjugates of gold nanorods and nanospheres for the detection of tumor antigens was described by Liu et al 111 Wang et al 112 provided data on the detection of hepatitis B virus in blood with gold nanorods conjugated to specific antibodies.…”

Preparation and application of various nanoparticles in biology and medicine

“…This approach was further applied by other researchers for detection of other antigens. [32][33][34] Sequence-specific methods for detection of polynucleotides are essential for diagnosis of genetic diseases and bacterial infection. Generally these methods are based on hybridization of immobilized target with complementary oligonucleotide probe bounded with reporter molecule.…”