Hen egg and fish egg phosvitins: composition and iron binding properties

Guérin-Dubiard, Catherine; Anton, Marc; Dhene-Garcia, Alexandre; Martinet, Virginie; Brulé, G.

doi:10.1007/s00217-001-0460-3

Cited by 26 publications

(27 citation statements)

References 1 publication

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“…All of them presented high Ser content, relatively dominant presence of Lys, Arg, Asp, Glu and His with respect to all residues but Ser, lack of Met, negligible quantities of Tyr, Cys and Trp. These features are in accordance with the literature, particularly for Ser content as it has been reported that hen phosvitin contains as low as 30% serine [1,2,28,36].…”

Section: Characterizations Of Pvt I and Pvt Fsupporting

confidence: 93%

“…When increasing the pH, a decrease in the purity of phosvitin was observed, as the contaminate proteins were increasing. The isoelectric point of phosvitin is around 4.0 [28]. The magnitude of net protein charge was increased as the pH increases, and thus the effect of phosvitin isolation decreased.…”

Section: Influence Of Peg6000 Concentration On Phosvitin Isolationmentioning

confidence: 99%

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Simply and effectively preparing high‐purity phosvitin using polyethylene glycol and anion‐exchange chromatography

Zhang

Qiu

Geng

et al. 2011

J of Separation Science

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An attempt was made to develop a new protocol for preparing phosvitin that could be easy scaled up using polyethylene glycol (PEG6000). Influence of PEG6000 concentration and pH of sample solution on phosvitin isolation was investigated. Phosvitin of high purity (99%) was obtained in good yield (47%) with the optimal condition of pH 4.0 and 3% PEG6000 precipitation. In addition, through evaluating different anion-exchange chromatography methods, the DEAE procedure at pH 7.5 was finally selected as the best procedure to obtain metal-free phosvitin that lost the least protein. Furthermore, it is observed that the purity and characterizations of prepared phosvitin were similar to those of the standard phosvitin from Sigma, and the random coil of phosvitin converted into more compact structure after removing metal ion. In conclusion, the developed method was simple and suitable for scaled up preparation of phosvitin.

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Section: Characterizations Of Pvt I and Pvt Fsupporting

confidence: 93%

Section: Influence Of Peg6000 Concentration On Phosvitin Isolationmentioning

confidence: 99%

Simply and effectively preparing high‐purity phosvitin using polyethylene glycol and anion‐exchange chromatography

Zhang

Qiu

Geng

et al. 2011

J of Separation Science

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“…Previously, we have shown that zebrafish rPv did not bind Fe 3+ [11], which is basically in line with the observation that fish Pv has a weaker iron binding capacity than hen Pv [6]. In this study we clearly showed that zebrafish rPv, like hen egg Pv, is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid system, and scavenging the DPPH radical, and more importantly, we demonstrated that zebrafish rPv is a potent antioxidant molecule that can protect radical-mediated oxidation of cellular biomolecules, indicating that zebrafish rPv may be useful for maintaining cellular integrity and normal biological functions.…”

Section: Discussionsupporting

confidence: 89%

“…For example, hen egg yolk phosvitin (Pv) has been isolated and its derived oligophosphopeptides show strong antioxidant activity owing to its high serine and phosphorus content, which makes this protein one of the strongest iron-chelating agents [6,7]. Hen egg Pv was also found to possess antithrombin activity [8], bactericidal activity against Escherichia coli [9], and endotoxinneutralizing activity [10].…”

Section: Introductionmentioning

confidence: 99%

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Zebrafish phosvitin is an antioxidant with non-cytotoxic activity

Hu¹,

Sun²,

Luan³

et al. 2015

ABBS

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Antioxidants, or anti-oxidant agents, have attracted a great deal of attention in recent years because of their roles in prevention of chronic diseases and utilization as preservatives in food and cosmetics. In this study, we clearly demonstrated that zebrafish recombinant phosvitin (rPv) is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid, and scavenging the 2,2-diphenyl-1-picrylhydrazyl radical. We also showed that zebrafish rPv is a cellular antioxidant capable of protecting radical-mediated oxidation of cellular biomolecules. Importantly, zebrafish rPv is non-cytotoxic to murine macrophage RAW264.7 cells. It is the first report that showed the antioxidant activities of Pv in fishes, suggesting that zebrafish Pv can be an important antioxidant, which can be used as preservatives in food and cosmetics and even as supplementary mediator in different diseased states.

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³¹P NMR assessment of the phosvitin‐iron complex in mayonnaise

Merkx

Delić

Wierenga

et al. 2018

Magnetic Reson in Chemistry

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Lipid oxidation is the main reason for the limited shelf life of mayonnaise. One of the main catalysts of this process is iron, which is introduced in its ferric (Fe(III)) form via phosvitin, an egg yolk phosphoprotein rich in phosphoserines. The binding of Fe(III) to phosvitin and its ability to establish a redox couple with Fe(II) is believed to determine the oxidation rate of unsaturated lipids. In this work, a 31P NMR based method was developed to quantify loading of phosvitin with Fe(III) and its reductive release. Both features could be quantified in model phosvitin solutions by exploiting the paramagnetic broadening of 31P NMR signal of phosphoserine residues by Fe(III). This method was then successfully applied to quantify the phosvitin‐Fe(III) loading in mayonnaise water phase by liquid NMR, whereas 31P NMR MAS could only provide a qualitative measure. The 31P NMR method showed a direct relation between loading of the Fe(III)‐phosvitin complex and lipid oxidation.

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Hen egg and fish egg phosvitins: composition and iron binding properties

Cited by 26 publications

References 1 publication

Simply and effectively preparing high‐purity phosvitin using polyethylene glycol and anion‐exchange chromatography

Simply and effectively preparing high‐purity phosvitin using polyethylene glycol and anion‐exchange chromatography

Zebrafish phosvitin is an antioxidant with non-cytotoxic activity

³¹P NMR assessment of the phosvitin‐iron complex in mayonnaise

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Hen egg and fish egg phosvitins: composition and iron binding properties

Cited by 26 publications

References 1 publication

Simply and effectively preparing high‐purity phosvitin using polyethylene glycol and anion‐exchange chromatography

Simply and effectively preparing high‐purity phosvitin using polyethylene glycol and anion‐exchange chromatography

Zebrafish phosvitin is an antioxidant with non-cytotoxic activity

31P NMR assessment of the phosvitin‐iron complex in mayonnaise

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Product

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³¹P NMR assessment of the phosvitin‐iron complex in mayonnaise